Long-Term in vitro Growth of Human T Cell Clones: Can Postmitotic ‘Senescent’ Cell Populations Be Defined?

1994 ◽  
Vol 104 (3) ◽  
pp. 232-239 ◽  
Author(s):  
Beatrix Grubeck-Loebenstein ◽  
Heidemarie Lechner ◽  
Klemens Trieb
Keyword(s):  
T Cell ◽  
Senescent Cell ◽  
Cell Populations ◽  
In Vitro Growth ◽  
T Cell Clones ◽  
Human T Cell ◽  
Cell Clones

scholarly journals Self-sparing of long-term in vitro-cloned or uncloned cytotoxic T lymphocytes.

1986 ◽  
Vol 164 (3) ◽  
pp. 962-967 ◽  
Author(s):  
M F Luciani ◽  
J F Brunet ◽  
M Suzan ◽  
F Denizot ◽  
P Golstein
Keyword(s):  
T Cell ◽  
T Lymphocytes ◽  
Cytotoxic T Lymphocytes ◽  
Cytotoxic T Cell ◽  
Cell Populations ◽  
Con A ◽  
T Cell Clones ◽  
Cell Clones

At least some long-term in vitro-cultured cytotoxic T cell clones and uncloned cell populations are able, in the presence of Con A, to lyse other cells, to be lysed by other cells, but not to lyse themselves. This as-yet-unexplained result may have implications as to the mechanism of T cell-mediated cytotoxicity.

Establishing T Cell Memory by Adoptive Transfer of T Cell Clones.

Blood ◽  
2006 ◽  
Vol 108 (11) ◽  
pp. 866-866
Author(s):  
Carolina Berger ◽  
Michael C. Jensen ◽  
Stanley R. Riddell
Keyword(s):  
T Cells ◽  
T Cell ◽  
Adoptive Transfer ◽  
Annexin V ◽  
T Cell Memory ◽  
T Cell Clones ◽  
Cell Clones

Abstract Adoptive transfer of T cells has been employed to reconstitute T cell immunity to viruses such as cytomegalovirus (CMV) in immunodeficient allogeneic stem cell transplant (SCT) patients and is being investigated to treat malignancies. In the allogeneic SCT setting, the T cells are derived from the donor and need to be isolated as clones or highly pure populations to avoid graft-versus-host disease. CD8+ T cells can be divided into defined subsets including CD62L− effector memory (TEM) and central memory T cells (TCM) expressing the CD62L lymph node homing molecule. Both TCM and TEM can give rise to cytolytic effector T cells (TE) after antigen stimulation and can be expanded in vitro for immunotherapy. However, the potential of T cells derived from either the TEM or TCM subset to persist in vivo has not been investigated. We used a macaque model to determine whether reconstitution of T cell memory to CMV by adoptive transfer of CD8+ T cell clones depended on their origin from either the CD62L+ TCM or CD62L− TEM subset. T cell clones were retrovirally transduced to express the macaque CD19 or CD20 surface marker to allow tracking of T cells in vivo. Clones derived from both TCM and TEM had similar avidity and proliferative capacity in vitro, and had a TE phenotype (CD62L−CCR7−CD28−CD127−, granzyme B+). TCM and TEM-derived T cell clones were transferred to macaques at doses of 3–6×108/kg and were both detected in the blood one day after transfer at 1.2–2.7% (low dose) to 20–25% (high dose) of CD8+ T cells. However, the frequency of TEM-derived T cells was undetectable after 3–5 days, and the cells were not present in lymph node or bone marrow obtained at day 14. By contrast, TCM-derived clones persisted in peripheral blood, migrated to tissue sites, and were detectable long-term at significant levels. A distinguishing feature of TCM-derived cells was their responsiveness to homeostatic cytokines. Only TCM-derived clones were rescued from apoptotic cell death by low-dose IL15 for >30 days in vitro and this correlated with higher levels of IL15Rα, IL2Rβ, and IL2Rγ, and of Bcl-xL and Bcl-2, which promote cell survival. To determine if the inability of TEM-derived clones to survive in vitro correlated with an increased susceptibility of cell death in vivo, we measured the proportion of infused cells that were positive for propidium iodide (PI) and Annexin V during the short period of in vivo persistence. One day after transfer, 41–45% of TEM-derived T cells were Annexin V+/PI+, analyzed directly in the blood or after 24 hours of culture. By contrast, only a minor fraction of an adoptively transferred TCM-derived T cell clone was Annexin V+/PI+ and the infused cells survived in vivo. A subset of the persisting T cells reacquired TCM marker (CD62L+CCR7+CD127+CD28+) in vivo and regained functional properties of TCM (direct lytic activity; rapid proliferation to antigen). These T cells produced IFN-γ and TNF-α after peptide stimulation, and studies are in progress to assess their in vivo response to antigen by delivery of T cells expressing CMV proteins. Our studies in a large animal model show for the first time that CD8+ TE derived from TCM but not TEM can persist long-term, occupy memory T cell niches, and restore TCM subsets of CMV-specific immunity. Thus, taking advantage of the genetic programming of cells that have become TCM might yield T cells with greater therapeutic activity and could be targeted for human studies of T cell therapy for both viral and malignant disease.

Effects of a reduced oxygen tension culture system on human T cell clones as a function of in vitro age

2004 ◽  
Vol 39 (4) ◽  
pp. 525-530 ◽  
Author(s):  
Orla Duggan ◽  
Paul Hyland ◽  
Kathryn Annett ◽  
Robin Freeburn ◽  
Christopher Barnett ◽  
...  
Keyword(s):  
T Cell ◽  
Oxygen Tension ◽  
Culture System ◽  
T Cell Clones ◽  
Human T Cell ◽  
Cell Clones

Killing of schistosomula of Schistosoma mansoni by macrophages: induction by T-cell clone-derived lymphokines and interferon-gamma

Parasitology ◽  
1986 ◽  
Vol 92 (2) ◽  
pp. 325-336 ◽  
Author(s):  
C. F. Kubelka ◽  
A. Ruppel ◽  
P. H. Krammer ◽  
D. Gemsa
Keyword(s):  
T Cell ◽  
Interferon Gamma ◽  
Macrophage Activation ◽  
Cell Clone ◽  
T Cell Clones ◽  
Cell Clones ◽  
T Cell Clone ◽  
Ifn Γ

SUMMARYThe induction of schistosomulicidal activity of peritoneal macrophages by concanavalin A-stimulated supernatants from long-term T-cell clones and by interferon-gamma (IFN-γ) was investigated in detail. Optimal conditions of in vitro macrophage activation by T-cell clone supernatants were established. Macrophages from 13-week S. mansoni-infected mice responded to lymphokine activation as well as resident mnacrophages from uninfecteci mice. IFN-γ was shown to play an essential role in induction of schistosomulicidal macrophage activity: recombinant IFN-γ at high concentration could induce schistosomula killing, and an anti-IFN-γ antiserum inhibited the induction ofschistosomulicidal activity by T-cell clone supernatants. Our data also indicate that macrophage activation could be obtained by IFN-γ in synergy with other lymphokines in the supernatant of long-term T-cell clones. Macrophages from mice injected with T-cell clone supernatants were primed in vivo and triggered to kill schistosomula in vitro in the presence of lipopolysaccharide (LPS). The data demonstrate that lymphokines produced by T-cell clones and, in particular, IFN-γ can participate in the activation of schistosomulicidal macrophages.

539 Interleukin 4 (IL-4) is responsible for IgE synthesis induced in vitro by human T cell clones

1988 ◽  
Vol 81 (1) ◽  
pp. 303 ◽  
Author(s):  
S. Romagnani ◽  
G.F. Del Prete ◽  
E. Maggi ◽  
P. Parronchi ◽  
A. Tiri ◽  
...  
Keyword(s):  
T Cell ◽  
Interleukin 4 ◽  
T Cell Clones ◽  
Human T Cell ◽  
Cell Clones ◽  
Ige Synthesis

scholarly journals Rabies Virus-specific Human T Cell Clones Provide Help for an in vitro Antibody Response against Neutralizing Antibody-inducing Determinants of the Viral Glycoprotein

1989 ◽  
Vol 70 (6) ◽  
pp. 1513-1521 ◽  
Author(s):  
H. Bunschoten ◽  
R. J. Klapmuts ◽  
I. J. Th. M. Claassen ◽  
S. D. Reyneveld ◽  
A. D. M. E. Osterhaus ◽  
...  
Keyword(s):  
T Cell ◽  
Antibody Response ◽  
Rabies Virus ◽  
Neutralizing Antibody ◽  
Viral Glycoprotein ◽  
T Cell Clones ◽  
Human T Cell ◽  
Cell Clones

Human T Cell Clones in Long-Term Culture as Models for the Impact of Chronic Antigenic Stress in Aging

2006 ◽  
pp. 781-792 ◽  
Author(s):  
Graham Pawelec ◽  
Erminia Mariani ◽  
Rafael Solana ◽  
Rosalyn Forsey ◽  
Anis Larbi ◽  
...  
Keyword(s):  
T Cell ◽  
T Cell Clones ◽  
Long Term Culture ◽  
Human T Cell ◽  
Cell Clones ◽  
The Impact

Interleukin-1 favours the in vitro development of type 2 T helper (Th2) human T-cell clones

1994 ◽  
Vol 145 (2) ◽  
pp. 93-100 ◽  
Author(s):  
R. Manetti ◽  
V. Barak ◽  
M.-P. Piccinni ◽  
S. Sampognaro ◽  
P. Parronchi ◽  
...  
Keyword(s):  
T Cell ◽  
Interleukin 1 ◽  
T Helper ◽  
T Cell Clones ◽  
In Vitro Development ◽  
Human T Cell ◽  
Cell Clones ◽  
Vitro Development

Alloproliferative human T cell clones primed and cultured in vitro lose proliferative and gain suppressive activity with age

1984 ◽  
Vol 10 (2) ◽  
pp. 135-142 ◽  
Author(s):  
G. Pawelec ◽  
P. Wernet ◽  
A. Rehbein ◽  
I. Balko ◽  
E.M. Schneider
Keyword(s):  
T Cell ◽  
Suppressive Activity ◽  
T Cell Clones ◽  
Human T Cell ◽  
Cell Clones
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