Effects of a reduced oxygen tension culture system on human T cell clones as a function of in vitro age

2004 ◽  
Vol 39 (4) ◽  
pp. 525-530 ◽  
Author(s):  
Orla Duggan ◽  
Paul Hyland ◽  
Kathryn Annett ◽  
Robin Freeburn ◽  
Christopher Barnett ◽  
...  
Keyword(s):  
T Cell ◽  
Oxygen Tension ◽  
Culture System ◽  
T Cell Clones ◽  
Human T Cell ◽  
Cell Clones

539 Interleukin 4 (IL-4) is responsible for IgE synthesis induced in vitro by human T cell clones

1988 ◽  
Vol 81 (1) ◽  
pp. 303 ◽  
Author(s):  
S. Romagnani ◽  
G.F. Del Prete ◽  
E. Maggi ◽  
P. Parronchi ◽  
A. Tiri ◽  
...  
Keyword(s):  
T Cell ◽  
Interleukin 4 ◽  
T Cell Clones ◽  
Human T Cell ◽  
Cell Clones ◽  
Ige Synthesis

scholarly journals Rabies Virus-specific Human T Cell Clones Provide Help for an in vitro Antibody Response against Neutralizing Antibody-inducing Determinants of the Viral Glycoprotein

1989 ◽  
Vol 70 (6) ◽  
pp. 1513-1521 ◽  
Author(s):  
H. Bunschoten ◽  
R. J. Klapmuts ◽  
I. J. Th. M. Claassen ◽  
S. D. Reyneveld ◽  
A. D. M. E. Osterhaus ◽  
...  
Keyword(s):  
T Cell ◽  
Antibody Response ◽  
Rabies Virus ◽  
Neutralizing Antibody ◽  
Viral Glycoprotein ◽  
T Cell Clones ◽  
Human T Cell ◽  
Cell Clones

Interleukin-1 favours the in vitro development of type 2 T helper (Th2) human T-cell clones

1994 ◽  
Vol 145 (2) ◽  
pp. 93-100 ◽  
Author(s):  
R. Manetti ◽  
V. Barak ◽  
M.-P. Piccinni ◽  
S. Sampognaro ◽  
P. Parronchi ◽  
...  
Keyword(s):  
T Cell ◽  
Interleukin 1 ◽  
T Helper ◽  
T Cell Clones ◽  
In Vitro Development ◽  
Human T Cell ◽  
Cell Clones ◽  
Vitro Development

Alloproliferative human T cell clones primed and cultured in vitro lose proliferative and gain suppressive activity with age

1984 ◽  
Vol 10 (2) ◽  
pp. 135-142 ◽  
Author(s):  
G. Pawelec ◽  
P. Wernet ◽  
A. Rehbein ◽  
I. Balko ◽  
E.M. Schneider
Keyword(s):  
T Cell ◽  
Suppressive Activity ◽  
T Cell Clones ◽  
Human T Cell ◽  
Cell Clones

scholarly journals Human T cell clones express functional homing receptors required for normal lymphocyte trafficking.

1985 ◽  
Vol 162 (3) ◽  
pp. 1075-1080 ◽  
Author(s):  
R F Navarro ◽  
S T Jalkanen ◽  
M Hsu ◽  
G Søenderstrup-Hansen ◽  
J Goronzy ◽  
...  
Keyword(s):  
T Cell ◽  
Lymphoid Tissues ◽  
Normal Lymphocyte ◽  
Lymphocyte Trafficking ◽  
High Endothelial Venules ◽  
T Cell Clones ◽  
Human T Cell ◽  
Cell Clones

To function efficiently in vivo, lymphocytes must circulate from the blood into lymphoid tissues and other sites of immune reaction. Herein, we show that human cytotoxic and helper T cell clones and lines, maintained in vitro with IL-2, express the functional capacity to recognize and bind to high endothelial venules (HEV), a capacity essential for lymphocyte exit from the blood, and hence for normal lymphocyte trafficking. The expression of functional homing receptors distinguishes human T cell clones from their murine counterparts, which uniformly lack receptors for HEV and are unable to migrate normally from the blood in vivo. The results raise the possibility that human T cell clones may be more effective in mediating in vivo immune responses than is suggested by murine models.

134 Characterization of human T cell clones that regulate IgE production in vitro

1985 ◽  
Vol 75 (1) ◽  
pp. 138
Author(s):  
B ZIMMERMAN ◽  
B UNDERDOWN ◽  
J ELLIS ◽  
O JAMES
Keyword(s):  
T Cell ◽  
T Cell Clones ◽  
Human T Cell ◽  
Cell Clones

scholarly journals Dissecting human T cell responses against Bordetella species.

1988 ◽  
Vol 168 (4) ◽  
pp. 1351-1362 ◽  
Author(s):  
M T De Magistris ◽  
M Romano ◽  
S Nuti ◽  
R Rappuoli ◽  
A Tagliabue
Keyword(s):  
T Cell ◽  
Whooping Cough ◽  
T Cell Memory ◽  
T Cell Clones ◽  
Cell Responses ◽  
Human T Cell ◽  
Cell Clones ◽  
Specific Antigens ◽  
New Generation

To identify the minimal structures that may be important for the creation of a synthetic and/or recombinant vaccine against whooping cough, human T cell clones were obtained against Bordetella antigens. Cloned peripheral blood T lymphocytes from an immune donor were grown in IL-2 and tested for proliferation in response to inactivated Bordetella species (B. pertussis, B. parapertussis, and B. bronchiseptica) and mutants deficient for the expression of virulence-associated antigens. All the T cell clones obtained were CD4+8- and recognized specifically the Bordetella antigens when presented by autologous B cells. On the basis of the responsiveness to the whole inactivated bacteria, it was possible to cluster the 12 clones obtained into four groups with the following specificity: (1) filamentous hemagglutinin (FHA); (2) B. pertussis-specific antigens; (3) virulence-associated Bordetella-specific antigens; and (4) nonvirulence-associated Bordetella-specific antigens. Using two new B. pertussis deletion mutants, clone 6 (representative of cluster 1) was found to recognize the COOH terminus of FHA. Furthermore, three out of four clones of cluster 3 were specifically stimulated by the soluble 69-kD protein from the outer membrane of B. pertussis. Surprisingly, none of the twelve clones obtained by stimulation in vitro with whole inactivated bacteria recognized pertussis toxin (PT), which is believed to be the most important protein to be included in an acellular vaccine. However, when a new generation of clones was obtained using soluble PT as the in vitro stimulus, it was observed that 11 clones of this group recognized this antigen. Thus, PT does not seem to be the most representative antigen on the whole inactivated bacteria, although T cell memory against PT exists in a donor who had the disease several years ago.

scholarly journals Self-sparing of long-term in vitro-cloned or uncloned cytotoxic T lymphocytes.

1986 ◽  
Vol 164 (3) ◽  
pp. 962-967 ◽  
Author(s):  
M F Luciani ◽  
J F Brunet ◽  
M Suzan ◽  
F Denizot ◽  
P Golstein
Keyword(s):  
T Cell ◽  
T Lymphocytes ◽  
Cytotoxic T Lymphocytes ◽  
Cytotoxic T Cell ◽  
Cell Populations ◽  
Con A ◽  
T Cell Clones ◽  
Cell Clones

At least some long-term in vitro-cultured cytotoxic T cell clones and uncloned cell populations are able, in the presence of Con A, to lyse other cells, to be lysed by other cells, but not to lyse themselves. This as-yet-unexplained result may have implications as to the mechanism of T cell-mediated cytotoxicity.

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