Effect of Cetirizine on Human Eosinophil Superoxide Generation, Eosinophil Chemotaxis and Eosinophil Peroxidase in vitro

1994 ◽  
Vol 103 (4) ◽  
pp. 384-390 ◽  
Author(s):  
Chiharu Okada ◽  
Ryosuke Eda ◽  
Hidefumi Miyagawa ◽  
Haruhito Sugiyama ◽  
Russell J. Hopp ◽  
...  
Keyword(s):  
Superoxide Generation ◽  
Human Eosinophil ◽  
Eosinophil Peroxidase ◽  
Eosinophil Chemotaxis

Modulation of Normal Human Eosinophil Chemotaxis in vitro by Herbimycin A, Erbstatin and Pervanadate

1998 ◽  
Vol 117 (1) ◽  
pp. 10-13 ◽  
Author(s):  
Amr El-Shazly ◽  
Keisuke Masuyama ◽  
Yasuhiro Samejima ◽  
Masao Eura ◽  
Takeru Ishikawa
Keyword(s):  
Human Eosinophil ◽  
Herbimycin A ◽  
Normal Human ◽  
Eosinophil Chemotaxis

Modulation of human eosinophil chemotaxis and adhesion by anti-allergic drugs in vitro

2008 ◽  
Vol 4 (S4) ◽  
pp. 13-18 ◽  
Author(s):  
R. Sehmi ◽  
G. M. Walsh ◽  
A. Hartnell ◽  
J. Barkans ◽  
J. North ◽  
...  
Keyword(s):  
Human Eosinophil ◽  
Eosinophil Chemotaxis

Inhibition of Human Eosinophil Chemotaxis in Vitro by the Anti-Allergic Agent Emedastine Difumarate

1996 ◽  
Vol 18 (4) ◽  
pp. 587-595 ◽  
Author(s):  
A. E. El-Shazly ◽  
K. Masuyama ◽  
Y. Samejima ◽  
M. Eura ◽  
T. Ishikawa
Keyword(s):  
Human Eosinophil ◽  
Eosinophil Chemotaxis

scholarly journals Role of cyclic GMP on inhibition by nitric oxide donors of human eosinophil chemotaxis in vitro

2004 ◽  
Vol 141 (4) ◽  
pp. 653-660 ◽  
Author(s):  
Sara M Thomazzi ◽  
Juliana Moreira ◽  
Sisi Marcondes ◽  
Gilberto De Nucci ◽  
Edson Antunes
Keyword(s):  
Nitric Oxide ◽  
Cyclic Gmp ◽  
Nitric Oxide Donors ◽  
Human Eosinophil ◽  
Eosinophil Chemotaxis

scholarly journals rhEPO (recombinant human eosinophil peroxidase): expression in Pichia pastoris and biochemical characterization

2006 ◽  
Vol 395 (2) ◽  
pp. 295-301 ◽  
Author(s):  
Chiara Ciaccio ◽  
Alessandra Gambacurta ◽  
Giampiero DE Sanctis ◽  
Domenico Spagnolo ◽  
Christina Sakarikou ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Biochemical Characterization ◽  
Expression System ◽  
Gel Filtration ◽  
Proteolytic Processing ◽  
Kinetic Properties ◽  
Specific Substrate ◽  
Human Eosinophil ◽  
Eosinophil Peroxidase ◽  
Pichia Pastoris Expression System

A Pichia pastoris expression system has for the first time been successfully developed to produce rhEPO (recombinant human eosinophil peroxidase). The full-length rhEPO coding sequence was cloned into the pPIC9 vector in frame with the yeast α-Factor secretion signal under the transcriptional control of the AOX (acyl-CoA oxidase) promoter, and transformed into P. pastoris strain GS115. Evidence for the production of rhEPO by P. pastoris as a glycosylated dimer precursor of approx. 80 kDa was determined by SDS/PAGE and gel filtration chromatography. Recombinant hEPO undergoes proteolytic processing, similar to that in the native host, to generate two chains of approx. 50 and 20 kDa. A preliminary biochemical characterization of purified rhEPO demonstrated that the spectral and kinetic properties of the recombinant wild-type EPO are comparable with those of the native enzyme and are accompanied by oxidizing activity towards several physiological anionic substrates such as SCN−, Br− and Cl−. On the basis of the estimated Km and kcat values it is evident that the pseudohalide SCN− is the most specific substrate for rhEPO, consistent with the catalytic properties of other mammalian EPOs purified from blood.

Effects of Cetirizine on Human Eosinophil and Neutrophil Activation in vitro

1991 ◽  
Vol 95 (2-3) ◽  
pp. 158-162 ◽  
Author(s):  
G.M. Walsh ◽  
R. Moqbel ◽  
A. Hartnell ◽  
A.B. Kay
Keyword(s):  
Neutrophil Activation ◽  
Human Eosinophil

scholarly journals Eosinophil Deficiency Compromises Lung Defense against Aspergillus fumigatus

2013 ◽  
Vol 82 (3) ◽  
pp. 1315-1325 ◽  
Author(s):  
Lauren M. Lilly ◽  
Michaella Scopel ◽  
Michael P. Nelson ◽  
Ashley R. Burg ◽  
Chad W. Dunaway ◽  
...  
Keyword(s):  
Aspergillus Fumigatus ◽  
Allergic Bronchopulmonary Aspergillosis ◽  
Cell Contact ◽  
Mrna Levels ◽  
Colony Stimulating Factor ◽  
Content Type ◽  
Eosinophil Peroxidase ◽  
Stimulating Factor ◽  
Deficient Mice

ABSTRACTExposure to the moldAspergillus fumigatusmay result in allergic bronchopulmonary aspergillosis, chronic necrotizing pulmonary aspergillosis, or invasive aspergillosis (IA), depending on the host's immune status. Neutrophil deficiency is the predominant risk factor for the development of IA, the most life-threatening condition associated withA. fumigatusexposure. Here we demonstrate that in addition to neutrophils, eosinophils are an important contributor to the clearance ofA. fumigatusfrom the lung. AcuteA. fumigatuschallenge in normal mice induced the recruitment of CD11b+Siglec F+Ly-6GloLy-6CnegCCR3+eosinophils to the lungs, which was accompanied by an increase in lungEpx(eosinophil peroxidase) mRNA levels. Mice deficient in the transcription factor dblGATA1, which exhibit a selective deficiency in eosinophils, demonstrated impairedA. fumigatusclearance and evidence of germinating organisms in the lung. Higher burden correlated with lower mRNA expression ofEpx(eosinophil peroxidase) andPrg2(major basic protein) as well as lower interleukin 1β (IL-1β), IL-6, IL-17A, granulocyte colony-stimulating factor (G-CSF), granulocyte-macrophage colony-stimulating factor (GM-CSF), and CXCL1 levels. However, examination of lung inflammatory cell populations failed to demonstrate defects in monocyte/macrophage, dendritic cell, or neutrophil recruitment in dblGATA1-deficient mice, suggesting that the absence of eosinophils in dlbGATA1-deficient mice was the sole cause of impaired lung clearance. We show that eosinophils generated from bone marrow have potent killing activity againstA. fumigtausin vitro, which does not require cell contact and can be recapitulated by eosinophil whole-cell lysates. Collectively, our data support a role for eosinophils in the lung response afterA. fumigatusexposure.

scholarly journals In vivo priming of platelet-activating factor-induced eosinophil chemotaxis in allergic asthmatic individuals

Blood ◽  
1992 ◽  
Vol 79 (7) ◽  
pp. 1836-1841 ◽  
Author(s):  
RA Warringa ◽  
HJ Mengelers ◽  
PH Kuijper ◽  
JA Raaijmakers ◽  
PL Bruijnzeel ◽  
...  
Keyword(s):  
Peripheral Blood ◽  
Platelet Activating Factor ◽  
Chemotactic Response ◽  
Healthy Individuals ◽  
Gm Csf ◽  
Asthmatic Patients ◽  
Allergic Asthmatic ◽  
Eosinophil Chemotaxis

Abstract The cytokines granulocyte-macrophage colony-stimulating factor (GM- CSF), interleukin (IL)-3, and IL-5 are important modulators of eosinophilia and eosinophil function. Eosinophil chemotaxis is known to be particularly sensitive for cytokine priming. In the present study, we compared chemotactic responses of eosinophils derived from peripheral blood of allergic asthmatics to responses of eosinophils from peripheral blood of healthy individuals. Eosinophils from allergic asthmatics exhibited a markedly increased sensitivity in their chemotactic response toward platelet-activating factor (PAF) compared with eosinophils from normal donors. In contrast, C5a-induced eosinophil chemotaxis between both groups was similar. This in vivo- primed phenotype could be mimicked in vitro, by preincubating eosinophils from peripheral blood of healthy individuals with picomolar concentrations of either GM-CSF, IL-3, or IL-5. The chemotactic response of eosinophils derived from the circulation of allergic asthmatic patients toward GM-CSF was significantly lower compared with the response of eosinophils of healthy individuals. Our data strongly suggest that release of cytokines may be an important in vivo priming mechanism for eosinophils in the circulation of allergic asthmatic patients. Such an in vivo priming can subsequently result in selective upregulation and downregulation of chemotactic responses toward various chemoattractants release in the lung tissue.

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