Effect of Mast Cell Chymase on the Morphology of Thyroid Cells in vitro

1992 ◽  
Vol 99 (1) ◽  
pp. 133-140 ◽  
Author(s):  
R. De Forteza ◽  
K. Banovac ◽  
E. Koren
Keyword(s):  
Mast Cell ◽  
Thyroid Cells ◽  
Mast Cell Chymase

scholarly journals Mast Cell Chymase Inhibits Smooth Muscle Cell Growth and Collagen Expression In Vitro

2001 ◽  
Vol 21 (12) ◽  
pp. 1928-1933 ◽  
Author(s):  
Yenfeng Wang ◽  
Naotaka Shiota ◽  
Markus J. Leskinen ◽  
Ken A. Lindstedt ◽  
Petri T. Kovanen
Keyword(s):  
Mast Cell ◽  
Smooth Muscle ◽  
Cell Growth ◽  
Smooth Muscle Cell ◽  
Muscle Cell ◽  
Collagen Expression ◽  
Smooth Muscle Cell Growth ◽  
Mast Cell Chymase

scholarly journals Distribution of chymase-containing mast cells in human bronchi.

1992 ◽  
Vol 40 (6) ◽  
pp. 781-786 ◽  
Author(s):  
R Matin ◽  
E K Tam ◽  
J A Nadel ◽  
G H Caughey
Keyword(s):  
Mast Cell ◽  
Smooth Muscle ◽  
Mast Cells ◽  
Airway Disease ◽  
Differential Distribution ◽  
Cell Subpopulations ◽  
Mast Cell Chymase ◽  
Tissue Compartments ◽  
Human Bronchi

Mast cell chymase stimulates secretion from cultured airway gland serous cells and hydrolyzes bronchoactive peptides in vitro. To explore the likelihood of these interactions occurring in situ, we examined the distribution and concentration of chymase-containing mast cells near glands and smooth muscle of major human bronchi from eight individuals without known airway disease. Total airway mast cells and the subset of mast cells containing chymase were detected by staining for methylene blue metachromasia and chloroacetate esterase activity, respectively. The percentage of chymase-containing mast cells was found to differ strikingly among bronchial tissue compartments. Near glands, for example, the concentration of chymase-positive mast cells (640 +/- 120 cells/mm3) was 73 +/- 9% that of total mast cells (910 +/- 130 cells/mm3), whereas in smooth muscle the concentration of chymase-positive mast cells (450 +/- 200 cells/mm3) was only 14 +/- 4% that of total mast cells (2920 +/- 620 cells/mm3). Of all chymase-containing mast cells in the airway subepithelium, 30 +/- 4% were located within 20 microns of submucosal glands. Although the percentage of chymase-containing cells varied, the absolute concentration of chymase-containing mast cells was similar in all compartments. These results reveal a differential distribution of mast cell subpopulations in human airway and suggest that mast cells containing chymase are near gland and smooth muscle targets.

Mast cell chymase is present in uterine cervical carcinoma and it detaches viable and growing cervical squamous carcinoma cells from substratum in vitro

2011 ◽  
Vol 303 (7) ◽  
pp. 499-512 ◽  
Author(s):  
Nicolae-Costin Diaconu ◽  
Jaana Rummukainen ◽  
Anita Naukkarinen ◽  
Mikko Mättö ◽  
Rauno J. Harvima ◽  
...  
Keyword(s):  
Mast Cell ◽  
Cervical Carcinoma ◽  
Squamous Carcinoma ◽  
Carcinoma Cells ◽  
Uterine Cervical Carcinoma ◽  
Squamous Carcinoma Cells ◽  
Mast Cell Chymase

scholarly journals Mast cell chymase affects the proliferation and metastasis of lung carcinoma cells in vitro

2017 ◽  
Vol 14 (3) ◽  
pp. 3193-3198 ◽  
Author(s):  
Yuan Jiang ◽  
Yudan Wu ◽  
William James Hardie ◽  
Xiaoying Zhou
Keyword(s):  
Mast Cell ◽  
Lung Carcinoma ◽  
Carcinoma Cells ◽  
Lung Carcinoma Cells ◽  
Proliferation And Metastasis ◽  
Mast Cell Chymase

Preincubation with Sn-complexes causes intensive intracellular retention of 99mTc in thyroid cells in vitro

2012 ◽  
Vol 51 (05) ◽  
pp. 179-185 ◽  
Author(s):  
M. Wendisch ◽  
D. Aurich ◽  
R. Runge ◽  
R. Freudenberg ◽  
J. Kotzerke ◽  
...  
Keyword(s):  
Cell Model ◽  
Low Energy ◽  
Thyroid Cells ◽  
Low Energy Electrons ◽  
A Cell ◽  
Vitro Model ◽  
Uptake Studies ◽  
Radiobiological Effects ◽  
Radioactivity Retention

SummaryTechnetium radiopharmaceuticals are well established in nuclear medicine. Besides its well-known gamma radiation, 99mTc emits an average of five Auger and internal conversion electrons per decay. The biological toxicity of these low-energy, high-LET (linear energy transfer) emissions is a controversial subject. One aim of this study was to estimate in a cell model how much 99mTc can be present in exposed cells and which radiobiological effects could be estimated in 99mTc-overloaded cells. Methods: Sodium iodine symporter (NIS)- positive thyroid cells were used. 99mTc-uptake studies were performed after preincubation with a non-radioactive (cold) stannous pyro - phosphate kit solution or as a standard 99mTc pyrophosphate kit preparation or with pure pertechnetate solution. Survival curves were analyzed from colony-forming assays. Results: Preincubation with stannous complexes causes irreversible intracellular radioactivity retention of 99mTc and is followed by further pertechnetate influx to an unexpectedly high 99mTc level. The uptake of 99mTc pertechnetate in NIS-positive cells can be modified using stannous pyrophosphate from 3–5% to >80%. The maximum possible cellular uptake of 99mTc was 90 Bq/cell. Compared with nearly pure extracellular irradiation from routine 99mTc complexes, cell survival was reduced by 3–4 orders of magnitude after preincubation with stannous pyrophosphate. Conclusions: Intra cellular 99mTc retention is related to reduced survival, which is most likely mediated by the emission of low-energy electrons. Our findings show that the described experiments constitute a simple and useful in vitro model for radiobiological investigations in a cell model.

scholarly journals FOXE1-Dependent Regulation of Macrophage Chemotaxis by Thyroid Cells In Vitro and In Vivo

2021 ◽  
Vol 22 (14) ◽  
pp. 7666
Author(s):  
Sara C. Credendino ◽  
Marta De Menna ◽  
Irene Cantone ◽  
Carmen Moccia ◽  
Matteo Esposito ◽  
...  
Keyword(s):  
Thyroid Cancer ◽  
Immune Cells ◽  
Cancer Susceptibility ◽  
Macrophage Infiltration ◽  
M2 Macrophage ◽  
Thyroid Cells ◽  
Transcriptional Alterations ◽  
Cancer Phenotypes

Forkhead box E1 (FOXE1) is a lineage-restricted transcription factor involved in thyroid cancer susceptibility. Cancer-associated polymorphisms map in regulatory regions, thus affecting the extent of gene expression. We have recently shown that genetic reduction of FOXE1 dosage modifies multiple thyroid cancer phenotypes. To identify relevant effectors playing roles in thyroid cancer development, here we analyse FOXE1-induced transcriptional alterations in thyroid cells that do not express endogenous FOXE1. Expression of FOXE1 elicits cell migration, while transcriptome analysis reveals that several immune cells-related categories are highly enriched in differentially expressed genes, including several upregulated chemokines involved in macrophage recruitment. Accordingly, FOXE1-expressing cells induce chemotaxis of co-cultured monocytes. We then asked if FOXE1 was able to regulate macrophage infiltration in thyroid cancers in vivo by using a mouse model of cancer, either wild type or with only one functional FOXE1 allele. Expression of the same set of chemokines directly correlates with FOXE1 dosage, and pro-tumourigenic M2 macrophage infiltration is decreased in tumours with reduced FOXE1. These data establish a novel link between FOXE1 and macrophages recruitment in the thyroid cancer microenvironment, highlighting an unsuspected function of this gene in the crosstalk between neoplastic and immune cells that shape tumour development and progression.

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