Induction of Allergen-Specific T Cells by Conjugates of N-Formyl-Methionyl-Leucyl-Phenylalanine and Rye Grass Pollen Extract

1988 ◽  
Vol 85 (1) ◽  
pp. 104-108
Author(s):  
R.M. Cook ◽  
A.W. Wheeler ◽  
V.M. Spackman ◽  
N.R.J. Musgrove ◽  
Y.K. Dave ◽  
...  
Keyword(s):  
T Cells ◽  
Grass Pollen ◽  
Pollen Extract ◽  
Rye Grass

Induction of T-Helper Cell Activity by Fragments of Rye Grass Pollen Extract Produced by Digestion with Chymotrypsin

1988 ◽  
Vol 87 (4) ◽  
pp. 337-341 ◽  
Author(s):  
R. Standring ◽  
E.A. Lavender ◽  
A.W. Wheeler ◽  
V.M. Spackman ◽  
D.M. Moran
Keyword(s):  
Grass Pollen ◽  
Cell Activity ◽  
T Helper Cell ◽  
Helper Cell ◽  
Pollen Extract ◽  
T Helper ◽  
Rye Grass

Murine Lymphocyte Responses to Purified Components of Rye Grass Pollen Extract

1985 ◽  
Vol 76 (3) ◽  
pp. 261-265
Author(s):  
D.M. Moran ◽  
G.P. Cottam ◽  
R. Standring ◽  
R.M. Cook
Keyword(s):  
Grass Pollen ◽  
Pollen Extract ◽  
Rye Grass ◽  
Murine Lymphocyte ◽  
Lymphocyte Responses

scholarly journals Physicochemical and immunochemical characterization of allergenic proteins from rye-grass (Lolium perenne) pollen prepared by a rapid and efficient purification method

1986 ◽  
Vol 234 (2) ◽  
pp. 305-310 ◽  
Author(s):  
G P Cottam ◽  
D M Moran ◽  
R Standring
Keyword(s):  
Amino Acid ◽  
Lolium Perenne ◽  
Grass Pollen ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Polyacrylamide Gel ◽  
Published Data ◽  
Pollen Extract ◽  
Rye Grass ◽  
Group I

Three fractions of rye-grass (Lolium perenne) pollen extract have been isolated by preparative isoelectric focusing (i.e.f.) and characterized in terms of physicochemical and immunochemical properties. The purified components were designated ‘R7’ and ‘R14’ on the basis of their positions in relation to other rye-grass pollen extract components on SDS/polyacrylamide-gel electrophoresis and their apparent molecular masses were assessed as 31 and 11 kDa respectively. On i.e.f., R14 split into two components, one acidic (pI 5.0) and one basic (pI 9.0), termed ‘R14a’ and ‘R14b’ respectively, and R7 focused at pI 5.8. R7 and R14a were shown to be allergenic by skin-prick test and all three components were recognized by rye-grass-pollen-specific human IgE. On SDS/polyacrylamide-gel electrophoresis and i.e.f., R7 behaved in a manner identical with that shown by an authentic sample of Rye I and gave an amino acid analysis similar to published data [Johnson & Marsh (1966) Immunochemistry 3, 91-100] for Rye group-I isoallergens; the amino acid sequence of the first 27 N-terminal amino acids was also determined. Physicochemical analysis revealed that R14a was equivalent to Rye II and 14b to Rye III. Preparative i.e.f. followed by gel-permeation chromatography proved to be a rapid and efficient method for purifying the allergenic components of Rye I (R7), Rye II (R14a) and Rye III (R14b) from rye-grass pollen extract.

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