Demonstration of Human, Rye Grass Pollen Extract-Specific, Helper and Suppressor Stimulating Activity of Modified Allergens by Effects on in vitro Hapten-Specific Antibody Production

1989 ◽  
Vol 90 (4) ◽  
pp. 320-325 ◽  
Author(s):  
A.W. Wheeler ◽  
S.G.O. Johansson
Keyword(s):  
Antibody Production ◽  
Specific Antibody ◽  
Grass Pollen ◽  
Pollen Extract ◽  
Rye Grass ◽  
Specific Antibody Production

scholarly journals Potentiating Antigen-Specific Antibody Production with Peptides Obtained from In Silico Screening for High-Affinity against MHC-II

Molecules ◽  
2019 ◽  
Vol 24 (16) ◽  
pp. 2949
Author(s):  
Yoshiro Hanyu ◽  
Yuto Komeiji ◽  
Mieko Kato
Keyword(s):  
Monoclonal Antibodies ◽  
Binding Affinity ◽  
Antibody Production ◽  
Specific Antibody ◽  
In Silico ◽  
Igg Antibody ◽  
Mhc Ii ◽  
High Affinity ◽  
Specific Antibody Production

Monoclonal antibodies with high affinity and specificity are essential for research and clinical purposes, yet remain difficult to produce. Agretope peptides that can potentiate antigen-specific antibody production have been reported recently. Here, we screened in silico for peptides with higher affinity against the agretope binding pocket in the MHC-II. The screening was based on the 3D crystal structure of a complex between MHC-II and a 14-mer peptide consisting of ovalbumin residues 323–339. Using this 14-mer peptide as template, we constructed a library of candidate peptides and screened for those that bound tightly to MHC-II. Peptide sequences that exhibited a higher binding affinity than the original ovalbumin peptide were identified. The peptide with the highest binding affinity was synthesized and its ability to boost antigen-specific antibody production in vivo and in vitro was assessed. In both cases, antigen-specific IgG antibody production was potentiated. Monoclonal antibodies were established by in vitro immunization using this peptide as immunostimulant, confirming the usefulness of such screened peptides for monoclonal antibody production.

scholarly journals In vitro immunization of Epstein–Barr virus-immortalized B cells augments antigen-specific antibody production

2013 ◽  
Vol 65 (6) ◽  
pp. 979-983 ◽  
Author(s):  
Gakuro Harada ◽  
Shin-ei Matsumoto ◽  
Makiko Yamashita ◽  
Kaoru Fujii ◽  
Sanetaka Shirahata ◽  
...  
Keyword(s):  
B Cells ◽  
Antibody Production ◽  
Specific Antibody ◽  
Epstein Barr Virus ◽  
In Vitro Immunization ◽  
Barr Virus ◽  
Specific Antibody Production ◽  
Epstein Barr

scholarly journals In vitro antigen-induced, antigen-specific antibody production in man. Specific and polyclonal components, kinetics, and cellular requirements.

1981 ◽  
Vol 154 (4) ◽  
pp. 1043-1057 ◽  
Author(s):  
H C Lane ◽  
D J Volkman ◽  
G Whalen ◽  
A S Fauci
Keyword(s):  
B Cell ◽  
Antibody Production ◽  
Specific Antibody ◽  
Cell Activation ◽  
Human Peripheral Blood ◽  
Culture Conditions ◽  
Culture Vessel ◽  
Cell Repertoire ◽  
Specific Antibody Production

A highly specific and reproducible antigen-induced, antigen-specific culture and assay system for antibody production by human peripheral blood B lymphocytes has been developed. The system is clearly T cell and monocyte dependent and is independent of exogenous mitogens. The major factors in our ability to trigger specific antibody production with antigen alone have been the use of extremely low concentrations of antigen in vitro (doses several orders of magnitude below those inducing a peak blastogenic response), careful attention to in vitro cell density and culture vessel geometry, and appreciation of the kinetics of the circulating antigen-inducible B cell repertoire. A dichotomy and overlap between antigen-induced, antigen-specific and antigen-induced, polyclonal responses was observed in the study of doubly immunized individuals. Whereas antibody responses highly specific for the antigen in culture were observed under one set of culture conditions (flat-bottomed vessels, 1.5 x 10(6) cells), switching to another culture system (round-bottomed vessels, 5 x 10(5) cells) resulted in polyclonal responses to antigen. Despite these culture condition-related differences in the induction of antibody synthesis, the suppression of specific antibody production that occurred at high concentrations of antigen was specific only for the antigen in culture. The capability to easily and reproducibly look at truly antigen-induced, antigen specific antibody production should be a major tool in furthering the understanding of human B cell activation and immunoregulation.

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