In vitro Effects of 13-Cis-Retinoic Acid (Ro 4-3780) and Etretinate (Ro 10-9359) on DNA Synthesis and Fine Structure of Guinea Pig and Human Lymphoid Cells

1983 ◽  
Vol 71 (4) ◽  
pp. 363-367 ◽  
Author(s):  
Klas Nordlind ◽  
Johan Thyberg
Keyword(s):  
Fine Structure ◽  
Retinoic Acid ◽  
Guinea Pig ◽  
Dna Synthesis ◽  
Lymphoid Cells ◽  
In Vitro Effects

Planarian regeneration: in vivo and in vitro effects of calcium and calmodulin on DNA synthesis

1983 ◽  
Vol 13 (1) ◽  
pp. 25-34 ◽  
Author(s):  
Isabelle Martelly ◽  
Annie Molla ◽  
Monique Thomasset ◽  
Albert Le Moigne
Keyword(s):  
Dna Synthesis ◽  
Planarian Regeneration ◽  
In Vitro Effects

scholarly journals Identity and Proliferation of Small Lymphocyte Precursors in Cultures of Lymphocyte-rich Fractions of Guinea Pig Bone Marrow

Blood ◽  
1971 ◽  
Vol 37 (1) ◽  
pp. 73-86 ◽  
Author(s):  
Y. YOSHIDA ◽  
D. G. OSMOND
Keyword(s):  
Bone Marrow ◽  
Guinea Pig ◽  
Lymphoid Cells ◽  
Short Term ◽  
Small Lymphocyte ◽  
Undifferentiated Cells ◽  
The Mean ◽  
High Concentrations ◽  
Transitional Cells

Abstract Radioautography with 3H-thymidine was used to examine the proliferative activity of bone marrow lymphoid cells and to identify the precursor cells of small lymphocytes in short-term cultures of lymphocyte-rich marrow fractions. High concentrations of small lymphocytes (nuclear diameters less than 8.0 µ in smears) together with large lymphoid ("transitional") cells (nuclear diameters greater than 8.0 µ) were separated from suspensions of guinea pig bone marrow by centrifugation in linear sucrose-serum density gradients. When such lymphocyte-rich marrow fractions were cultured in vitro the labeling and mitotic indices following either continuous or terminal exposure to 3H-thymidine indicated that the large lymphoid cells were confined mainly to the pre-DNA-synthetic (G1) and early DNA-synthetic (S) phases at first, but proceeded subsequently through S phase and mitosis. From these data tentative values were derived for the in vitro duration of G1 (12 hours) and S (13.7 hours). Further cultures were followed radioautographically after a 1-hour pulse of 3H-thymidine at 6-7 hours of culture. The absolute numbers of labeled large lymphoid cells declined during the subsequent 21 hours but, simultaneously, labeled small lymphocytes appeared and increased progressively in absolute numbers to 44.4 ± 8.1 per cent of the initial numbers of labeled large lymphoid cells. The mean grain count of labeled small lymphocytes was half that of the initially labeled large lymphoid cells. Very few labeled undifferentiated cells other than large lymphoid cells were observed. The results demonstrate that lymphocyte-rich marrow fractions are capable of sustaining the production of small lymphocytes in short-term cultures and that the immediate precursors of marrow small lymphocytes are contained within a population of large lymphoid cells.

In vitro Effects of retinoic acid on mouse incisor development

1994 ◽  
Vol 39 (10) ◽  
pp. 891-900 ◽  
Author(s):  
Agnès Bloch-Zupan ◽  
Manuel P. Mark ◽  
Bruno Weber ◽  
Jean Victor Ruch
Keyword(s):  
Retinoic Acid ◽  
In Vitro Effects ◽  
Mouse Incisor ◽  
Incisor Development
Sign in / Sign up

Export Citation Format

Share Document