Further Characterization of the Neutrophil-Derived Eosinophil Chemotactic Factor and Comparison to Eosinophil Chemotactic Activity from Mast Cells

1981 ◽  
Vol 66 (1) ◽  
pp. 172-173 ◽  
Author(s):  
B.M. Czarnetzki ◽  
R.E. Zimmermann ◽  
N. Fischer
Keyword(s):  
Mast Cells ◽  
Chemotactic Activity ◽  
Chemotactic Factor ◽  
Eosinophil Chemotactic Factor

scholarly journals Biological characterization of purified macrophage-derived neutrophil chemotactic factor

1995 ◽  
Vol 4 (4) ◽  
pp. 263-269 ◽  
Author(s):  
M. Dias-Baruffi ◽  
M. C. Roque-Barreira ◽  
F. Q. Cunha ◽  
S. H. Ferreira
Keyword(s):  
Neutrophil Migration ◽  
Chemotactic Activity ◽  
Chemotactic Factor ◽  
Biological Characterization ◽  
Important Mediator ◽  
Neutrophil Chemotactic Factor ◽  
Chemotactic Gradient

We have recently described the purification of a 54 kDa acidic protein, identified as macrophage-derived neutrophil chemotactic factor (MNCF). This protein causesin vitrochemotaxis as well asin vivoneutrophil migration even in animals treated with dexamethasone. Thisin vivochemotactic activity of MNCF in animals pretreated with dexamethasone is an uncommon characteristic which discriminates MNCF from known chemotactic cytokines. MNCF is released in the supernatant by macrophage monolayers stimulated with lipopolysaccharide (LPS). In the present study, we describe some biological characteristics of homogenous purified MNCF. When assayedin vitro, MNCF gave a bell-shaped dose–response curve. Thisin vitroactivity was shown to be caused by haptotaxis. Unlike N-formyl-methionylleucyl- phenylalanine (FMLP) or interleukin 8 (IL-8), the chemotactic activity of MNCFin vivoandin vitro, was inhibited by preincubation with D-galactose but not with D-mannose. In contrast with IL-8, MNCF did not bind to heparin and antiserum against IL-8 was ineffective in inhibiting its chemotactic activity. These data indicate that MNCF induces neutrophil migration through a carbohydrate recognition property, but by a mechanism different from that of the known chemokines. It is suggested that MNCF may be an important mediator in the recruitment of neutrophils via the formation of a substrate bound chemotactic gradient (haptotaxis) in the inflamed tissues.

Characterization of Citrullination Sites in Neutrophils and Mast Cells Activated by Ionomycin via Integration of Mass Spectrometry and Machine Learning

2021 ◽  
Author(s):  
Raghothama Chaerkady ◽  
Yebin Zhou ◽  
Jared A. Delmar ◽  
Shao Huan Samuel Weng ◽  
Junmin Wang ◽  
...  
Keyword(s):  
Machine Learning ◽  
Mass Spectrometry ◽  
Mast Cells
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