Isolation and characterization of a neutrophil chemotactic factor fromTritrichomonas foetusorganisms

1994 ◽  
Vol 72 (3) ◽  
pp. 249-255 ◽  
Author(s):  
M. OWHASHI ◽  
F. TOMIYOSHI ◽  
H. HAYASHI
Keyword(s):  
Chemotactic Factor ◽  
Isolation And Characterization ◽  
Neutrophil Chemotactic Factor

scholarly journals Characterization of a receptor for human monocyte-derived neutrophil chemotactic factor/interleukin-8.

1990 ◽  
Vol 265 (14) ◽  
pp. 8311-8316
Author(s):  
P M Grob ◽  
E David ◽  
T C Warren ◽  
R P DeLeon ◽  
P R Farina ◽  
...  
Keyword(s):  
Human Monocyte ◽  
Chemotactic Factor ◽  
Interleukin 8 ◽  
Neutrophil Chemotactic Factor

Production and Characterization of Recombinant Human Neutrophil Chemotactic Factor

1989 ◽  
Vol 106 (3) ◽  
pp. 436-441 ◽  
Author(s):  
Ryuji Furuta ◽  
Junichi Yamagishi ◽  
Hirotada Kotani ◽  
Fumiko Sakamoto ◽  
Toshikazu Fukui ◽  
...  
Keyword(s):  
Human Neutrophil ◽  
Chemotactic Factor ◽  
Neutrophil Chemotactic Factor

scholarly journals Biological characterization of purified macrophage-derived neutrophil chemotactic factor

1995 ◽  
Vol 4 (4) ◽  
pp. 263-269 ◽  
Author(s):  
M. Dias-Baruffi ◽  
M. C. Roque-Barreira ◽  
F. Q. Cunha ◽  
S. H. Ferreira
Keyword(s):  
Neutrophil Migration ◽  
Chemotactic Activity ◽  
Chemotactic Factor ◽  
Biological Characterization ◽  
Important Mediator ◽  
Neutrophil Chemotactic Factor ◽  
Chemotactic Gradient

We have recently described the purification of a 54 kDa acidic protein, identified as macrophage-derived neutrophil chemotactic factor (MNCF). This protein causesin vitrochemotaxis as well asin vivoneutrophil migration even in animals treated with dexamethasone. Thisin vivochemotactic activity of MNCF in animals pretreated with dexamethasone is an uncommon characteristic which discriminates MNCF from known chemotactic cytokines. MNCF is released in the supernatant by macrophage monolayers stimulated with lipopolysaccharide (LPS). In the present study, we describe some biological characteristics of homogenous purified MNCF. When assayedin vitro, MNCF gave a bell-shaped dose–response curve. Thisin vitroactivity was shown to be caused by haptotaxis. Unlike N-formyl-methionylleucyl- phenylalanine (FMLP) or interleukin 8 (IL-8), the chemotactic activity of MNCFin vivoandin vitro, was inhibited by preincubation with D-galactose but not with D-mannose. In contrast with IL-8, MNCF did not bind to heparin and antiserum against IL-8 was ineffective in inhibiting its chemotactic activity. These data indicate that MNCF induces neutrophil migration through a carbohydrate recognition property, but by a mechanism different from that of the known chemokines. It is suggested that MNCF may be an important mediator in the recruitment of neutrophils via the formation of a substrate bound chemotactic gradient (haptotaxis) in the inflamed tissues.

Molecular cloning and characterization of a novel neutrophil chemotactic factor from a filarial parasite

1993 ◽  
Vol 30 (14) ◽  
pp. 1315-1320 ◽  
Author(s):  
Owhashi Makoto ◽  
Futaki Shiroh ◽  
Kitagawa Kouki ◽  
Horii Yoichiro ◽  
Maruyama Haruhiko ◽  
...  
Keyword(s):  
Molecular Cloning ◽  
Chemotactic Factor ◽  
Filarial Parasite ◽  
Neutrophil Chemotactic Factor

scholarly journals Isolation and partial chemical characterization of macrophage-derived neutrophil chemotactic factor

1995 ◽  
Vol 4 (4) ◽  
pp. 257-262 ◽  
Author(s):  
M. Dias-Baruffi ◽  
M. C. Roque-Barreira ◽  
F. Q. Cunha ◽  
S. H. Ferreira
Keyword(s):  
Neutrophil Migration ◽  
Gel Filtration ◽  
Chemical Characterization ◽  
Chemotactic Factor ◽  
Sds Page ◽  
Neutrophil Chemotactic Factor ◽  
Partial Chemical

Macrophages stimulated with lipopolysaccharide (LPS) release a factor (MNCF; macrophage-derived neutrophil chemotactic factor) which induces neutrophil migrationin vivoandin vitro. Thein vivochemotactic activity of crude MNCF is not affected by pretreating the animals with dexamethasone, an uncommon characteristic which discriminates MNCF from known chemotactic cytokines. We purified MNCF by affinity chromatography of the supernatant from LPS-stimulated macrophages on immobilized D-galactose, followed by gel filtration of the sugar-binding material on Superdex 75. The activity was eluted in the volume corresponding to a MW of 54 kDa. SDS–PAGE of this preparation revealed a single band, also corresponding to a 54 kDa protein. MNCF is an acidic protein (pI < 4) as shown by chromatofocussing. Like the crude MNCF, the homogeneous protein induced neutrophil migrationin vitroas well asin vivo. This was not modified by dexamethasone pretreatment.

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