Effect of Long-Term Cyclophosphamide Treatment on T and B Lymphocytes in Normal Guinea Pigs

1978 ◽  
Vol 56 (5) ◽  
pp. 443-448 ◽  
Author(s):  
P.A. Revell
Keyword(s):  
B Lymphocytes ◽  
Guinea Pigs ◽  
T And B Lymphocytes ◽  
Cyclophosphamide Treatment

Determination of the number of T and B lymphocytes in guinea pigs

1982 ◽  
Vol 94 (3) ◽  
pp. 1262-1265
Author(s):  
M. Ya. Shvartsman ◽  
A. P. Kashkin
Keyword(s):  
B Lymphocytes ◽  
Guinea Pigs ◽  
T And B Lymphocytes

Electron Microscopic Observations on T and B Lymphocytes from Spleens of Syngeneic Radiation Chimaeras

1976 ◽  
Vol 62 (5) ◽  
pp. 503-515
Author(s):  
Simonetta Rebessi ◽  
Benito Di Caterino ◽  
Luciano Adorini ◽  
Vincenzo Covelli
Keyword(s):  
B Lymphocytes ◽  
T And B Lymphocytes ◽  
Morphological Observation ◽  
Immune Reactivity ◽  
Electron Microscopic ◽  
Cytotoxicity Assays ◽  
Morphological Differences ◽  
Lymphocyte Populations ◽  
Very High

The ultrastructure of T and B lymphocytes has been examined in long-term syngeneic chimaeras and age-control mice. Spleen cell suspensions from these mice were passed through glass wool columns to obtain pure lymphocyte populations. These cells were then separated into T and B lymphocytes by nylon wool columns, and their purity was tested by cytotoxicity assays with anti-ϑ serum. Electron microscopic observations on such separated T and B lymphocytes did not reveal morphological differences except when the cells were fully differentiated, either as mature (T2) cells or plasmacells. In particular, T2 cells showed a very high cytoplasmic density, attributable to the presence of a larger number of microfilaments with respect to immature (T1) cells. In long-term chimaeras a significantly larger number of T2 cells was found as compared to age-control mice, and this morphological observation is correlated with the differences in immune reactivity and leukemia incidence previously described in these mice.

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