Determination of the number of T and B lymphocytes in guinea pigs

1982 ◽  
Vol 94 (3) ◽  
pp. 1262-1265
Author(s):  
M. Ya. Shvartsman ◽  
A. P. Kashkin
Keyword(s):  
B Lymphocytes ◽  
Guinea Pigs ◽  
T And B Lymphocytes

scholarly journals ОСОБЛИВОСТІ ІМУННОГО ГОМЕОСТАЗУ В ДИНАМІЦІ РОЗВИТКУ ЕКСПЕРИМЕНТАЛЬНОЇ БРОНХІАЛЬНОЇ АСТМИ

2018 ◽  
Author(s):  
M. S. Reheda ◽  
L. A. Lubinets ◽  
B. F. Shchepanskyi
Keyword(s):  
Immune System ◽  
B Lymphocytes ◽  
Immune Complexes ◽  
Guinea Pigs ◽  
Circulating Immune Complexes ◽  
System Response ◽  
Control Group ◽  
Modeling Process ◽  
Immune System Response

In this paper, it is shown that the modeling process of bronchial asthma (BA ) is accompanied by changes, in comparison with the control group, in the indicators of immune system response: T- & B-lymphocytes, circulating immune complexes in blood of guinea pig males on the 4th, 18th, 25th day of experiment.The aim of the study – determination of some indices of immune system in blood of guinea pigs in the modeling process of BA on 4th, 18th, 25th day of the experiment.Materials and Methods. Experiments were conducted on 40 guinea pigs (males), with body weight 0.25–0.27 kg. Animals were divided into four groups of ten animals in each. Intact guinea pigs were the first group. Animals with experimental BA – the second, third, fourth group respectively on the 4th, 18th, 25th day of the experiment. Experimental BA was reproduced by V. I. Babych method. In blood of intact guinea pigs and animals with experimental BA , the number of T- and B-lymphocytes was determined by the method of E. F. Chernushenko, L. S. Kohosov, determination of the level of circulating immune complexes was carried out by the method of V. Haskova and co-authors. The results of the study were processed by the method of variation statistics using Student's criterion.Results and Discussion. The results of the studies showed unidirectional changes in individual parameters of the immune system, depending on the periods of the formation of BA : an increase in the number of B-lymphocytes and circulating immune complexes, a decrease in the number of T-lymphocytes for all of the studied days of the experiment.Conclusions. The obtained results indicate significant changes in the immune system parameters in the blood of experimental animals with BA and are important for understanding the pathogenesis of BA . These studies provide an opportunity to find the more perfect and effective methods of diagnosis of BA.

Parallel determination of mineralocorticoid and glucocorticoid receptors in T- and B-lymphocytes of human spleen

1988 ◽  
Vol 118 (4) ◽  
pp. 479-482 ◽  
Author(s):  
D. Armanini ◽  
S. Endres ◽  
U. Kuhnle ◽  
P. C. Weber
Keyword(s):  
B Lymphocytes ◽  
Glucocorticoid Receptors ◽  
Human Lymphocytes ◽  
T And B Lymphocytes ◽  
Mineralocorticoid Receptors ◽  
Human Spleen ◽  
Apparent Dissociation Constant ◽  
Binding Characteristics ◽  
Number Of Binding Sites

Abstract. The binding characteristics of glucocorticoid and mineralocorticoid receptors were determined in T-and B-lymphocytes from the spleen of four deceased kidney donors. The number of glucocorticoid and mineralocorticoid receptors was equivalent in T- and B-cells (respectively, 1308 ± 364 and 1335 ± 520 glucocorticoid receptors per cell, and 174 ± 29 and 164 ± 24 mineralocorticoid receptors per cell). The number of binding sites per cell was 5- to 10-fold higher for dexamethasone than for aldosterone. The apparent dissociation constant of dexamethasone for glucocorticoid receptors was 2-fold higher than that of aldosterone for mineralocorticoid receptors. In conclusion, subpopulations of human lymphocytes have an equal number of glucocorticoid and mineralocorticoid receptors.

Relationship among T- and B-lymphocytes of the peripheral blood and thyroid tissue in patients with Graves' disease

2019 ◽  
Author(s):  
Margarita A Dudina ◽  
Andrey A Savchenko ◽  
Sergey A Dogadin ◽  
Alexandr G Borisov ◽  
Igor V Kudryavcev ◽  
...  
Keyword(s):  
B Lymphocytes ◽  
Peripheral Blood ◽  
Thyroid Tissue ◽  
T And B Lymphocytes ◽  
Graves Disease
Sign in / Sign up

Export Citation Format

Share Document