Chemical Modification of Crude Timothy Grass Pollen Extract

1976 ◽  
Vol 50 (6) ◽  
pp. 693-708 ◽  
Author(s):  
D.M. Moran ◽  
A.W. Wheeler
Keyword(s):  
Chemical Modification ◽  
Grass Pollen ◽  
Pollen Extract ◽  
Timothy Grass ◽  
Timothy Grass Pollen

Chemical Modification of Crude Timothy Grass Pollen Extract

1977 ◽  
Vol 54 (4) ◽  
pp. 315-321 ◽  
Author(s):  
D.M. Moran ◽  
A.W. Wheeler ◽  
B.G. Overell ◽  
S.R. Woroniecki
Keyword(s):  
Chemical Modification ◽  
Grass Pollen ◽  
Pollen Extract ◽  
Timothy Grass ◽  
Timothy Grass Pollen

Chemical Modification of Crude Timothy Grass Pollen Extract

1976 ◽  
Vol 50 (6) ◽  
pp. 709-728 ◽  
Author(s):  
A.W. Wheeler ◽  
Patricia M. Jenkins ◽  
D.M. Moran
Keyword(s):  
Chemical Modification ◽  
Grass Pollen ◽  
Pollen Extract ◽  
Timothy Grass ◽  
Timothy Grass Pollen

Clinical efficacy of microencapsulated timothy grass pollen extract in grass-allergic individuals

2004 ◽  
Vol 92 (1) ◽  
pp. 25-31 ◽  
Author(s):  
Elizabeth C. TePas ◽  
Elisabeth G. Hoyte ◽  
Jennifer J. McIntire ◽  
Dale T. Umetsu
Keyword(s):  
Clinical Efficacy ◽  
Grass Pollen ◽  
Pollen Extract ◽  
Timothy Grass ◽  
Timothy Grass Pollen

scholarly journals Critical appraisal of Timothy grass pollen extract GRAZAX® in the management of allergic rhinitis

2015 ◽  
pp. 5897 ◽  
Author(s):  
Alessandra Scaparrotta ◽  
Marina Attanasi ◽  
Marianna Petrosino ◽  
Paola Di Filippo ◽  
Sabrina Di Pillo ◽  
...  
Keyword(s):  
Allergic Rhinitis ◽  
Grass Pollen ◽  
Critical Appraisal ◽  
Pollen Extract ◽  
Timothy Grass ◽  
Timothy Grass Pollen

Timothy grass pollen extract-induced gene expression and signalling pathways in airway epithelial cells

2011 ◽  
Vol 41 (6) ◽  
pp. 830-841 ◽  
Author(s):  
K I. L. Röschmann ◽  
S. Luiten ◽  
M. J. Jonker ◽  
T. M. Breit ◽  
W. J. Fokkens ◽  
...  
Keyword(s):  
Gene Expression ◽  
Epithelial Cells ◽  
Grass Pollen ◽  
Airway Epithelial Cells ◽  
Signalling Pathways ◽  
Pollen Extract ◽  
Airway Epithelial ◽  
Timothy Grass ◽  
Timothy Grass Pollen

scholarly journals Development of a protein microarray-based diagnostic chip mimicking the skin prick test for allergy diagnosis

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Marina Kalli ◽  
Andrew Blok ◽  
Long Jiang ◽  
Nichola Starr ◽  
Marcos J. C. Alcocer ◽  
...  
Keyword(s):  
Protein Stability ◽  
Grass Pollen ◽  
Cell Activation ◽  
Leukemia Cell ◽  
Protein Microarrays ◽  
Pollen Extract ◽  
Allergy Diagnosis ◽  
Timothy Grass ◽  
Timothy Grass Pollen ◽  
Coated Surfaces

Abstract Protein microarrays have been successfully used for detection of allergen-specific IgE in patient sera. Here, we demonstrate proof-of-concept of a solid-phase technique coupling the high-throughput potential of protein microarrays with the biologically relevant readout provided by IgE reporter cells, creating a novel allergic sensitization detection system. Three proteins (κ-casein, timothy grass pollen extract, polyclonal anti-human IgE) were printed onto three different polymer-coated surfaces (aldehyde-, epoxy- and NHS ester-coated). ToF–SIMs analysis was performed to assess printed protein stability and retention during washing steps. NFAT-DsRed rat basophil leukemia cell attachment and retention during washing steps was assessed after treatment with various extracellular matrix proteins. NFAT-DsRed IgE reporter cells were sensitized with serum of an allergic donor, incubated on the printed slides, and cell activation determined using a microarray laser scanner. NFAT DsRed IgE reporter cell binding was significantly increased on all polymer surfaces after incubation with fibronectin and vitronectin, but not collagen or laminin. All surfaces supported printed protein stability during washing procedure, with epoxy- and NHS ester-coated surfaces showing best protein retention. Cell activation was significantly higher in NHS ester-coated slides after timothy grass pollen extract stimulation appearing a suitable substrate for further development of an automated allergy diagnosis system.

Chemical modification of timothy grass pollen antigen B—I.

1979 ◽  
Vol 16 (11) ◽  
pp. 929-934 ◽  
Author(s):  
A. Malley ◽  
D. Begley ◽  
A. Forsham
Keyword(s):  
Chemical Modification ◽  
Grass Pollen ◽  
Timothy Grass ◽  
Antigen B ◽  
Timothy Grass Pollen
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