Isolation of allergenic molecules from a crude phleum pratense (timothy grass) pollen extract by combination of preparative size exclusion HPLC and preparative isoelectricfocusing

1988 ◽  
Vol 25 (3) ◽  
pp. 242-242 ◽  
Author(s):  
D. Meineke ◽  
R. Wahl ◽  
H. J. Maasch
Keyword(s):  
Grass Pollen ◽  
Phleum Pratense ◽  
Size Exclusion ◽  
Pollen Extract ◽  
Timothy Grass ◽  
Timothy Grass Pollen ◽  
Size Exclusion Hplc

scholarly journals Sensitization to timothy grass pollen allergenic molecules in children

2013 ◽  
Vol 8 ◽  
Author(s):  
Alessandra Scaparrotta ◽  
Marcello Verini ◽  
Nicola Pietro Consilvio ◽  
Anna Cingolani ◽  
Daniele Rapino ◽  
...  
Keyword(s):  
Grass Pollen ◽  
Pollen Allergy ◽  
Phleum Pratense ◽  
Pollen Extract ◽  
Mediterranean Population ◽  
Grass Pollen Allergy ◽  
Timothy Grass ◽  
Timothy Grass Pollen ◽  
Species Specific

Background: Grass pollens are significant elicitors of IgE-mediated allergic disease in the world and timothy (Phleum pratense) is one of the most important pollens of the family. Molecular and biochemical characterization of Phleum pratense has revealed several allergen components: rPhl p 1 and rPhl p 5 have been shown to be “Species Specific Allergens”, while the profilin rPhl p 12 and the calcium-binding protein rPhl p 7 are the principal Cross-Reactive components. Methods: In this study the pattern of sensitization to rPhl p 1, rPhl p 5, rPhl p 7 and rPhl p 12 was analyzed in children with asthma and/or rhinoconjunctivitis and grass pollen allergy, in order to evaluate the frequency of sensitization to allergenic molecules of Phleum pratense among pediatric subjects allergic to grass pollen in a Mediterranean population. The correlation of sensitization to these Phleum allergenic molecules with IgE against grass pollen extract and its variation according to age and level of IgE against grass pollen extract were evaluated. Results: IgE against to rPhl p 1 were found in 99% (205/207) of patients, to rPhl p 5 in 67% (139/207), to rPhl p 12 in 32% (66/207) and to rPhl p 7 only in 5% (10/207). Sensitization only to “Species Specific” (rPhl p1, rPhl p5) allergenic molecules of Phleum pratense was detected in 65% (135/207) of children. Our data show the predominant role of rPhl p 1 in pediatric populations as the most relevant sensitizing allergen detectable at all ages and at all levels of timothy grass pollen-specific IgE antibodies, while the importance of rPhl p 5 rises with the increase of patients’ age and with grass pollen IgE levels. Conclusions: The assessment of sensitization to grass pollen allergenic molecules could help develop a better characterization of allergic sensitization in grass pollen allergy in children, which may be different in every patient. It could also enable clinicians to give more specific and effective immunotherapy, based on allergenic molecule sensitization.

Clinical efficacy of microencapsulated timothy grass pollen extract in grass-allergic individuals

2004 ◽  
Vol 92 (1) ◽  
pp. 25-31 ◽  
Author(s):  
Elizabeth C. TePas ◽  
Elisabeth G. Hoyte ◽  
Jennifer J. McIntire ◽  
Dale T. Umetsu
Keyword(s):  
Clinical Efficacy ◽  
Grass Pollen ◽  
Pollen Extract ◽  
Timothy Grass ◽  
Timothy Grass Pollen

scholarly journals Critical appraisal of Timothy grass pollen extract GRAZAX® in the management of allergic rhinitis

2015 ◽  
pp. 5897 ◽  
Author(s):  
Alessandra Scaparrotta ◽  
Marina Attanasi ◽  
Marianna Petrosino ◽  
Paola Di Filippo ◽  
Sabrina Di Pillo ◽  
...  
Keyword(s):  
Allergic Rhinitis ◽  
Grass Pollen ◽  
Critical Appraisal ◽  
Pollen Extract ◽  
Timothy Grass ◽  
Timothy Grass Pollen

Chemical Modification of Crude Timothy Grass Pollen Extract

1977 ◽  
Vol 54 (4) ◽  
pp. 315-321 ◽  
Author(s):  
D.M. Moran ◽  
A.W. Wheeler ◽  
B.G. Overell ◽  
S.R. Woroniecki
Keyword(s):  
Chemical Modification ◽  
Grass Pollen ◽  
Pollen Extract ◽  
Timothy Grass ◽  
Timothy Grass Pollen

Pollen Sensitization in Association with Allergic Rhinitis in preschool asthmatic Children

QJM ◽  
2021 ◽  
Vol 114 (Supplement_1) ◽  
Author(s):  
Elham M Hossny ◽  
Hanan M Abd El-Lateef ◽  
Hadeer FM Salama
Keyword(s):  
Allergic Rhinitis ◽  
Grass Pollen ◽  
Skin Prick Testing ◽  
Cross Sectional Study ◽  
Negative Control ◽  
Phleum Pratense ◽  
Cross Sectional ◽  
Asthmatic Children ◽  
Timothy Grass ◽  
Timothy Grass Pollen

Abstract Background Grass pollens are significant elicitors of IgE-mediated allergic disease in the world and timothy grass (Phleum pratense) is an important pollen in allergy induction. Objective We sought to determine the rate of pollen sensitization in group of Egyptian asthmatic children with allergic rhinitis using timothy grass pollen as a potential triggering factor for allergic rhinitis. Patients and Methods This analytical cross sectional study was conducted on a group of preschool children following up at the Pediatric Allergy and Immunology Clinic, Children's Hospital, Ain Shams University during the period from December 2018 to April 2019. We enrolled 52 asthmatic children with concomitant allergic rhinitis sampled in a consecutive manner. They were subjected to skin prick testing for timothy grass pollen, positive histamine control and negative control. Results The study revealed high frequency of timothy grass pollen(TGP) sensitization occurring in 60.3% of patients with concomitant allergic rhinitis .wheal diameters pointed to sensitization only rather than actual allergy to TGP. TGP sensitization showed no significant correlation to disease duration. Children with rhinosinsuitis had comparable rates of TGP sensitization to those without rhinosinsuitis. Conclusion It is concluded from our results that sensitization to timothy grass pollen (TGP) is common in allergic rhinitis. The findings are limited by the sample size and consecutive manner of sampling. Wider scale studies are needed to accurately evaluate the frequency of timothy grass pollen (TGP) sensitization in our country.

Timothy grass pollen extract-induced gene expression and signalling pathways in airway epithelial cells

2011 ◽  
Vol 41 (6) ◽  
pp. 830-841 ◽  
Author(s):  
K I. L. Röschmann ◽  
S. Luiten ◽  
M. J. Jonker ◽  
T. M. Breit ◽  
W. J. Fokkens ◽  
...  
Keyword(s):  
Gene Expression ◽  
Epithelial Cells ◽  
Grass Pollen ◽  
Airway Epithelial Cells ◽  
Signalling Pathways ◽  
Pollen Extract ◽  
Airway Epithelial ◽  
Timothy Grass ◽  
Timothy Grass Pollen

scholarly journals Development of a protein microarray-based diagnostic chip mimicking the skin prick test for allergy diagnosis

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Marina Kalli ◽  
Andrew Blok ◽  
Long Jiang ◽  
Nichola Starr ◽  
Marcos J. C. Alcocer ◽  
...  
Keyword(s):  
Protein Stability ◽  
Grass Pollen ◽  
Cell Activation ◽  
Leukemia Cell ◽  
Protein Microarrays ◽  
Pollen Extract ◽  
Allergy Diagnosis ◽  
Timothy Grass ◽  
Timothy Grass Pollen ◽  
Coated Surfaces

Abstract Protein microarrays have been successfully used for detection of allergen-specific IgE in patient sera. Here, we demonstrate proof-of-concept of a solid-phase technique coupling the high-throughput potential of protein microarrays with the biologically relevant readout provided by IgE reporter cells, creating a novel allergic sensitization detection system. Three proteins (κ-casein, timothy grass pollen extract, polyclonal anti-human IgE) were printed onto three different polymer-coated surfaces (aldehyde-, epoxy- and NHS ester-coated). ToF–SIMs analysis was performed to assess printed protein stability and retention during washing steps. NFAT-DsRed rat basophil leukemia cell attachment and retention during washing steps was assessed after treatment with various extracellular matrix proteins. NFAT-DsRed IgE reporter cells were sensitized with serum of an allergic donor, incubated on the printed slides, and cell activation determined using a microarray laser scanner. NFAT DsRed IgE reporter cell binding was significantly increased on all polymer surfaces after incubation with fibronectin and vitronectin, but not collagen or laminin. All surfaces supported printed protein stability during washing procedure, with epoxy- and NHS ester-coated surfaces showing best protein retention. Cell activation was significantly higher in NHS ester-coated slides after timothy grass pollen extract stimulation appearing a suitable substrate for further development of an automated allergy diagnosis system.

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