scholarly journals Intramuscular Immunization with a Plasmid DNA Vaccine Encoding prM-E Protein from Japanese Encephalitis Virus: Enhanced Immunogenicity by Co-Administration of GM-CSF Gene and Genetic Fusions of prM-E Protein and GM-CSF

Intervirology ◽  
2009 ◽  
Vol 52 (3) ◽  
pp. 152-163 ◽  
Author(s):  
Yong-zhen Zhai ◽  
Xi-mei Li ◽  
Yan Zhou ◽  
Li Ma ◽  
Guo-he Feng
Keyword(s):  
Japanese Encephalitis Virus ◽  
Dna Vaccine ◽  
Japanese Encephalitis ◽  
Plasmid Dna ◽  
Encephalitis Virus ◽  
Gm Csf ◽  
E Protein ◽  
Plasmid Dna Vaccine

Immunologic Analysis Induced by DNA Vaccine Encoding E Protein of Beijing-1 Strain Derived from Japanese Encephalitis Virus

Intervirology ◽  
2006 ◽  
Vol 50 (2) ◽  
pp. 93-98 ◽  
Author(s):  
Guo-he Feng ◽  
Ning Liu ◽  
Yan Zhou ◽  
Yong-zhen Zhai ◽  
Xi-mei Li ◽  
...  
Keyword(s):  
Japanese Encephalitis Virus ◽  
Dna Vaccine ◽  
Japanese Encephalitis ◽  
Encephalitis Virus ◽  
E Protein ◽  
Immunologic Analysis

Development of an effective Japanese encephalitis virus-specific DNA vaccine

2006 ◽  
Vol 8 (11) ◽  
pp. 2578-2586 ◽  
Author(s):  
Chang Jer Wu ◽  
Tsung Lin Li ◽  
Hui Wen Huang ◽  
Mi Hua Tao ◽  
Yi Lin Chan
Keyword(s):  
Japanese Encephalitis Virus ◽  
Dna Vaccine ◽  
Japanese Encephalitis ◽  
Encephalitis Virus

scholarly journals Mutation analysis of the cross-reactive epitopes of Japanese encephalitis virus envelope glycoprotein

2012 ◽  
Vol 93 (6) ◽  
pp. 1185-1192 ◽  
Author(s):  
Shyan-Song Chiou ◽  
Yi-Chin Fan ◽  
Wayne D. Crill ◽  
Ruey-Yi Chang ◽  
Gwong-Jen J. Chang
Keyword(s):  
Amino Acid ◽  
Japanese Encephalitis Virus ◽  
Japanese Encephalitis ◽  
Recombinant Expression ◽  
Encephalitis Virus ◽  
Site Directed Mutagenesis ◽  
Amino Acid Residues ◽  
Virus Envelope ◽  
E Protein ◽  
Domain Iii

Group and serocomplex cross-reactive epitopes have been identified in the envelope (E) protein of several flaviviruses and have proven critical in vaccine and diagnostic antigen development. Here, we performed site-directed mutagenesis across the E gene of a recombinant expression plasmid that encodes the Japanese encephalitis virus (JEV) premembrane (prM) and E proteins and produces JEV virus-like particles (VLPs). Mutations were introduced at I135 and E138 in domain I; W101, G104, G106 and L107 in domain II; and T305, E306, K312, A315, S329, S331, G332 and D389 in domain III. None of the mutant JEV VLPs demonstrated reduced activity to the five JEV type-specific mAbs tested. Substitutions at W101, especially W101G, reduced reactivity dramatically with all of the flavivirus group cross-reactive mAbs. The group and JEV serocomplex cross-reactive mAbs examined recognized five and six different overlapping epitopes, respectively. Among five group cross-reactive epitopes, amino acids located in domains I, II and III were involved in one, five and three epitopes, respectively. Recognition by six JEV serocomplex cross-reactive mAbs was reduced by amino acid substitutions in domains II and III. These results suggest that amino acid residues located in the fusion loop of E domain II are the most critical for recognition by group cross-reactive mAbs, followed by residues of domains III and I. The amino acid residues of both domains II and III of the E protein were shown to be important in the binding of JEV serocomplex cross-reactive mAbs.

scholarly journals Complete protection for mice conferred by a DNA vaccine based on Japanese encephalitis virus P3 strain that is used to prepare the inactivated vaccine in China

2020 ◽  
Author(s):  
Ran Wang ◽  
Xiaozheng Yu ◽  
Yan Wang ◽  
Xiaoyan Zheng
Keyword(s):  
Japanese Encephalitis Virus ◽  
Dna Vaccine ◽  
Japanese Encephalitis ◽  
Protective Efficacy ◽  
Neutralizing Antibody ◽  
Encephalitis Virus ◽  
Inactivated Vaccine ◽  
Complete Protection ◽  
Igg Antibody ◽  
Humoral Immune

Abstract Background The incidence of Japanese encephalitis (JE) has been dramatically reduced in China after the coverage of the vaccine. It is believed that the live-attenuated Japanese encephalitis virus (JEV) vaccine SA14-14-2 has contributed a lot. Another vaccine that seems to have faded out of the public is an inactivated vaccine based on the JEV P3 strain, which is still considered to have certain modifiability, such as being transformed into a DNA vaccine to improve its immunogenicity. Methods In this study, the protective efficacy induced by a Japanese encephalitis DNA vaccine candidate pV-JP3ME encoding pre-membrane (prM) and envelope (E) proteins of P3 strain in BALB/c mice. The prM/E genes of the JEV P3 strain were subcloned into vector pVAX1 (pV) to construct pV-JP3ME. Results The plasmid DNA was immunized BALB/c mice, high titers of IgG antibody and neutralizing antibody (nAb) against JEV were detected. The key cytokines in splenocytes upon stimulation with JEV antigens were secreted. Finally, complete protective efficacy was generated after challenge with the JEV P3 strain in mice. Conclusions The DNA vaccine pV-JP3ME based on JEV P3 strain in this study can induce specific humoral immune and cytokine responses in mice, and provide complete protection for mice against JEV.

scholarly journals Establishment and Characterization of Japanese Encephalitis Virus-Specific, Human CD4+ T-Cell Clones: Flavivirus Cross-Reactivity, Protein Recognition, and Cytotoxic Activity

1998 ◽  
Vol 72 (10) ◽  
pp. 8032-8036 ◽  
Author(s):  
Hirokuni Aihara ◽  
Tomohiko Takasaki ◽  
Takaji Matsutani ◽  
Ryuji Suzuki ◽  
Ichiro Kurane
Keyword(s):  
T Cell ◽  
T Lymphocytes ◽  
Cytotoxic Activity ◽  
Japanese Encephalitis Virus ◽  
Japanese Encephalitis ◽  
Encephalitis Virus ◽  
T Cell Clones ◽  
E Protein ◽  
Hla Restriction ◽  
Cell Clones

ABSTRACT We analyzed the CD4+ T-lymphocyte responses of two donors who had received Japanese encephalitis virus (JEV) vaccine 6 or 12 months earlier. Bulk culture proliferation assays showed that peripheral blood mononuclear cells (PBMC) responded to JEV antigens (Ag) but also responded at lower levels to West Nile virus (WNV) and dengue virus type 1, 2, and 4 (D1V, D2V, and D4V, respectively) Ag. Five JEV-specific CD4+ human T-cell clones and one subclone were established from PBMC of these two donors. Two clones responded to WNV Ag as well as to JEV Ag, whereas the others responded only to JEV Ag. Three of five CD4+ T-cell clones had JEV-specific cytotoxic activity and recognized E protein. The HLA restriction of the JEV-specific T-cell clones was examined. Three clones were HLA-DR4 restricted, one was HLA-DQ3 restricted, and the HLA restriction of one clone was not determined. T-cell receptor analysis showed that these clones expressed different T-cell receptors, suggesting that they originated from different T lymphocytes. These results indicate that JEV vaccine induces JEV-specific and flavivirus-cross-reactive CD4+ T lymphocytes and that these T lymphocytes recognize E protein. The functions and HLA restriction patterns of these T lymphocytes are, however, heterogeneous.

scholarly journals Characterization of a serine-to-asparagine substitution at position 123 in the Japanese encephalitis virus E protein

2013 ◽  
Vol 94 (1) ◽  
pp. 90-96 ◽  
Author(s):  
Yukie Yamaguchi ◽  
Yoko Nukui ◽  
Akira Kotaki ◽  
Kyoko Sawabe ◽  
Masayuki Saijo ◽  
...  
Keyword(s):  
Japanese Encephalitis Virus ◽  
Japanese Encephalitis ◽  
Amino Acid Position ◽  
Encephalitis Virus ◽  
Viral Growth ◽  
E Protein ◽  
Growth Properties ◽  
Mosquito Cell Lines

Amino acid position 123 in the E protein of Japanese encephalitis virus (JEV) determines viral growth properties and pathogenicity. The majority of JEV strains have a serine residue at this position (E123S); however, JEV with an asparagine residue (E123N) has also been isolated. To compare the growth properties and pathogenicity of E123S and E123N JEV, we produced recombinant JEV with a serine-to-asparagine substitution at position 123 (rJEV-Mie41-ES123N) in the E123S-type strain Mie/41/2002 background. The growth rate of rJEV-Mie41-ES123N was similar to that of Mie/41/2002 in mammalian and mosquito cell lines. Mouse challenge experiments showed that there was only a slight difference in neuroinvasiveness between the parent strain (Mie/41/2002) and rJEV-Mie41-ES123N. Thus, our results indicate that the Ser-to-Asn substitution in the JEV E protein has weak impact on viral growth properties in vitro or on pathogenicity in vivo.

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