Development of an effective Japanese encephalitis virus-specific DNA vaccine

2006 ◽  
Vol 8 (11) ◽  
pp. 2578-2586 ◽  
Author(s):  
Chang Jer Wu ◽  
Tsung Lin Li ◽  
Hui Wen Huang ◽  
Mi Hua Tao ◽  
Yi Lin Chan
Keyword(s):  
Japanese Encephalitis Virus ◽  
Dna Vaccine ◽  
Japanese Encephalitis ◽  
Encephalitis Virus

scholarly journals Complete protection for mice conferred by a DNA vaccine based on Japanese encephalitis virus P3 strain that is used to prepare the inactivated vaccine in China

2020 ◽  
Author(s):  
Ran Wang ◽  
Xiaozheng Yu ◽  
Yan Wang ◽  
Xiaoyan Zheng
Keyword(s):  
Japanese Encephalitis Virus ◽  
Dna Vaccine ◽  
Japanese Encephalitis ◽  
Protective Efficacy ◽  
Neutralizing Antibody ◽  
Encephalitis Virus ◽  
Inactivated Vaccine ◽  
Complete Protection ◽  
Igg Antibody ◽  
Humoral Immune

Abstract Background The incidence of Japanese encephalitis (JE) has been dramatically reduced in China after the coverage of the vaccine. It is believed that the live-attenuated Japanese encephalitis virus (JEV) vaccine SA14-14-2 has contributed a lot. Another vaccine that seems to have faded out of the public is an inactivated vaccine based on the JEV P3 strain, which is still considered to have certain modifiability, such as being transformed into a DNA vaccine to improve its immunogenicity. Methods In this study, the protective efficacy induced by a Japanese encephalitis DNA vaccine candidate pV-JP3ME encoding pre-membrane (prM) and envelope (E) proteins of P3 strain in BALB/c mice. The prM/E genes of the JEV P3 strain were subcloned into vector pVAX1 (pV) to construct pV-JP3ME. Results The plasmid DNA was immunized BALB/c mice, high titers of IgG antibody and neutralizing antibody (nAb) against JEV were detected. The key cytokines in splenocytes upon stimulation with JEV antigens were secreted. Finally, complete protective efficacy was generated after challenge with the JEV P3 strain in mice. Conclusions The DNA vaccine pV-JP3ME based on JEV P3 strain in this study can induce specific humoral immune and cytokine responses in mice, and provide complete protection for mice against JEV.

scholarly journals A poorly neutralizing IgG2a/c response elicited by a DNA vaccine protects mice against Japanese encephalitis virus

2014 ◽  
Vol 95 (9) ◽  
pp. 1983-1990 ◽  
Author(s):  
Hsin-Wei Chen ◽  
Hui-Wen Huang ◽  
Hui-Mei Hu ◽  
Han-Hsuan Chung ◽  
Szu-Hsien Wu ◽  
...  
Keyword(s):  
Japanese Encephalitis Virus ◽  
Dna Vaccine ◽  
Japanese Encephalitis ◽  
Neutralizing Antibody ◽  
Encephalitis Virus ◽  
Igg Subclass ◽  
Lethal Challenge ◽  
Significant Difference ◽  
Ifn Γ ◽  
Deficient Mice

We demonstrated previously that immunization with a DNA vaccine expressing the Japanese encephalitis virus (JEV) envelope (E) protein conferred a high level of protection through a poorly neutralizing antibody response. Here, we further investigated the role of the IgG subclass in this antibody-dependent protection using cytokine co-immunization and cytokine-deficient mice. A significant difference in IgG2a/c but not IgG1 was observed between mice that survived or died following a lethal challenge. Correspondingly, the IgG2a/c response and protection increased in IL-4-deficient mice but decreased in IFN-γ-deficient mice, highlighting the importance of IgG2a/c. In addition, the restoration of protection and E-specific IgG2a/c production in IFN-γ-deficient mice by a T helper (Th) type 1-biased intramuscular immunization suggested that IgG2a/c but not IFN-γ was the major component for protection. The failure of protection against a direct intracranial challenge indicated that IgG2a/c-mediated protection was restricted to outside the central nervous system. Consistent with this conclusion, passive transfer of E-specific antisera conferred protection only pre-exposure to JEV. Therefore, our data provided evidence that the IgG subclass plays an important role in protection against JEV, particular in poorly neutralizing E-specific antibodies, and Th1-biased IgG2a/c confers better protection than Th2-biased IgG1 against JEV.

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