Determination of Red-Cell Volume with a Nonradioactive Marker (Sodium Fluorescein) in Humans Using Flow Cytometry

1997 ◽  
Vol 24 (3) ◽  
pp. 132-137
Author(s):  
M. Haller ◽  
H. Dressel ◽  
M. Thiel ◽  
V. Orth ◽  
H. Brechtelsbauer ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Cell Volume ◽  
Sodium Fluorescein ◽  
Red Cell ◽  
Red Cell Volume

Experience with sodium fluorescein flow cytometry in the determination of red cell volume

2009 ◽  
Vol 31 (2) ◽  
pp. 233-235 ◽  
Author(s):  
R. D. SLIGHT ◽  
R. FERGUSON ◽  
D. STIRLING ◽  
D. B. L. McCLELLAND ◽  
P. S. MANKAD
Keyword(s):  
Flow Cytometry ◽  
Cell Volume ◽  
Sodium Fluorescein ◽  
Red Cell ◽  
Red Cell Volume

Quantitative measurement of erythropoiesis in the dog

1960 ◽  
Vol 198 (1) ◽  
pp. 183-186 ◽  
Author(s):  
S. M. Weissman ◽  
T. A. Waldmann ◽  
N. I. Berlin
Keyword(s):  
Life Span ◽  
Cell Survival ◽  
Cell Volume ◽  
Quantitative Measurement ◽  
Red Cells ◽  
Red Cell ◽  
Red Cell Volume ◽  
Cell Life ◽  
Red Cell Survival

The quantitative measurement of erythropoiesis requires the simultaneous determination of total red cell volume, rate of production of red cells and the red cell life span. The total red cell volume was measured with autologous Cr51-labeled red cells, the rate of production of red cells from the rate of disappearance of radioiron from the plasma and uptake by red cells, the red cell life span with C14-labeled glycine and the apparent red cell survival T1/2 with Cr51. The average total red cell volume of the dogs studied was 38.6 cc/kg; the plasma radioiron T1/2 was 66 minutes; the red cell radio-iron uptake was 80%; the serum iron was 102 µg/100 cc, and the plasma volume calculated from the peripheral hematocrit and total red cell volume was 46 cc/kg, and from the extrapolation to t0 of the radioiron disappearance was 48 cc/kg. From these figures the plasma iron turnover was calculated to be 0.63 mg/kg/day and the red cell iron renewal rate 1.26%/day. The average red cell life span was 108 days; the average apparent T1/2 of Cr51 red cell survival was 24.3 days; the average elution rate of Cr51 was 1.77%/day.

Experiences with the Radiochromium method for Determination of Red Cell Volume

1962 ◽  
Vol 14 (4) ◽  
pp. 355-367 ◽  
Author(s):  
R. Wennesland ◽  
E. Brown ◽  
J. Hopper ◽  
K. G. Scott ◽  
J. L. Hodges ◽  
...  
Keyword(s):  
Cell Volume ◽  
Red Cell ◽  
Red Cell Volume

Mixing of Labeled Erythrocytes in the Dog's Spleen

1958 ◽  
Vol 195 (3) ◽  
pp. 628-630 ◽  
Author(s):  
L. Kraintz ◽  
J. de Boer ◽  
E. L. Smith ◽  
R. A. Huggins
Keyword(s):  
Cell Volume ◽  
General Circulation ◽  
Mixing Time ◽  
Red Cells ◽  
Red Cell ◽  
Spleen Size ◽  
Blood Samples ◽  
Red Cell Volume ◽  
Minute Period

Dogs anesthetized with morphine-pentobarbital were injected with Cr51-tagged red cells, and their mixing time in the general circulation and the spleen was compared. The necessary blood samples were taken and the spleens rapidly excised at 10, 20 or 30 minutes after the injection of the tagged red cells. When the data were grouped according to the size of the spleen and irrespective of the time of removal, the mixing of tagged red cells in small spleens was practically the same as in the general circulation. With increasing sizes of spleens the mixing appeared to be progressively less complete than in the general circulation. If the spleen size was disregarded and the data grouped according to the time after the injections of tagged red cells mixing in the spleen was still incomplete after 30 minutes. However, the maximum mean possible error that could be introduced into the determination of total red cell volume if all the unmixed red cells were ejected into the general circulation would be less than 5% at the 20-minute period.

First Clinical Implications of Perioperative Red Cell Volume Measurement with a Nonradioactive Marker (Sodium Fluorescein)

1998 ◽  
Vol 87 (6) ◽  
pp. 1234-1238 ◽  
Author(s):  
Victoria H. Orth ◽  
Markus Rehm ◽  
Manfred Thiel ◽  
Uwe Kreimeier ◽  
Mathias Haller ◽  
...  
Keyword(s):  
Cell Volume ◽  
Volume Measurement ◽  
Sodium Fluorescein ◽  
Clinical Implications ◽  
Red Cell ◽  
Red Cell Volume

Determination of red cell volume in infants needing blood transfusion

2000 ◽  
Vol 10 (3) ◽  
pp. 219-224 ◽  
Author(s):  
Fisher ◽  
Matthes ◽  
Wynn ◽  
Al-Ismail ◽  
Hoy ◽  
...  
Keyword(s):  
Blood Transfusion ◽  
Cell Volume ◽  
Red Cell ◽  
Red Cell Volume

scholarly journals First Clinical Implications of Perioperative Red Cell Volume Measurement with a Nonradioactive Marker (Sodium Fluorescein)

1998 ◽  
Vol 87 (6) ◽  
pp. 1234-1238 ◽  
Author(s):  
Victoria H. Orth ◽  
Markus Rehm ◽  
Manfred Thiel ◽  
Uwe Kreimeier ◽  
Mathias Haller ◽  
...  
Keyword(s):  
Cell Volume ◽  
Volume Measurement ◽  
Sodium Fluorescein ◽  
Clinical Implications ◽  
Red Cell ◽  
Red Cell Volume
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