Age-Related Changes in the mRNA Expression of Actin Isoforms in Drosophila melanogaster

Gerontology ◽  
1997 ◽  
Vol 43 (5) ◽  
pp. 261-267 ◽  
Author(s):  
Martin Labuhn ◽  
Christine Brack
Keyword(s):  
Mrna Expression ◽  
Actin Isoforms ◽  
Age Related ◽  
Age Related Changes ◽  
Drosophila Melanogaste

Age-related changes in D1 and D2 receptor mRNA expression in postmortem human putamen with and without multiple small infarcts

1998 ◽  
Vol 243 (1-3) ◽  
pp. 37-40 ◽  
Author(s):  
Hideo Tohgi ◽  
Kimiaki Utsugisawa ◽  
Masahiro Yoshimura ◽  
Yuriko Nagane ◽  
Masatoshi Mihara
Keyword(s):  
Mrna Expression ◽  
D2 Receptor ◽  
Receptor Mrna ◽  
Age Related ◽  
Age Related Changes

scholarly journals P102 Alternative splicing of the SLCO1B1 transporter in paediatric liver

2019 ◽  
Vol 104 (6) ◽  
pp. e60.1-e60
Author(s):  
BD van Groen ◽  
C Bi ◽  
R Gaedigk ◽  
V Staggs ◽  
D Tibboel ◽  
...  
Keyword(s):  
Alternative Splicing ◽  
Mrna Expression ◽  
Liver Tissue ◽  
Post Mortem ◽  
Mrna Isoforms ◽  
Age Related ◽  
Mrna Transcripts ◽  
Relationship Of ◽  
Novel Isoforms ◽  
Age Related Changes

BackgroundAlternative mRNA transcripts occur in >90% of human genes and may be triggered by developmental signals. The hepatic transporter OATP1B1 (gene name SLCO1B1) traffics substrates across the hepatic membrane, and shows age-related changes in protein expression. We aimed to predict novel isoforms of OATP1B1 by studying alternative splicing of SLCO1B1 in human paediatric post-mortem liver tissue, and the relationship of their mRNA expression with age.Methods mRNA expression of SLCO1B1 transcripts was determined using RNA sequencing (HISAT2/StringTie). Novel mRNA transcripts were considered of relevance when (1) the expression was >5% of the annotated isoform, (2) it was a SLCO1B7 and SCLO1B1 hybrid transcript, or (3) when the expression was associated with age. The software packages ORF-finder, TMpred and TOPO2 were used to predict the protein sequence and structure of the novel isoforms. Relationship of expression with age was studied with the Kruskal-Wallis test for age groups (fetal, 0–1.5 year, 1.5–6 year, 6–12 year and 12–18 year) and with Spearman correlation tests for age on continuous scale.ResultsIn 97 hepatic post-mortem tissues (gestational age median 16.4 [range 14.7–41.3] weeks, postnatal age 0.36 [0 - 17] years) 27 novel mRNA transcripts were detected. Of these, 13 were relevant: 2 isoforms are predicted to translate into amino acid sequences similar to the annotated isoform for OATP1B1, 9 isoforms may translate into truncated versions, and the expression of 8 isoforms was associated with age. None of the isoforms had an ORF that covered the SLCO1B7 region.ConclusionWe showed that novel SLCO1B1 mRNA isoforms potentially translate into OATP1B1 protein with unknown function, and that alternative splicing may well be a regulatory mechanism for SLCO1B1 expression during development. This data provides a better understanding of age-related changes in the expression of OATP1B1, and, with that, potentially improves prediction of disposition of endogenous and exogenous substrates.Disclosure(s)BG was supported, in part, by the Ter Meulen fund 2018 provided by the Royal Dutch Academy of Sciences. The National Institute of Child Health and Human Development Brain and Tissue Bank for Developmental Disorders at the University of Maryland is funded by the National Institutes of Health (NIH) contract HHSN275200900011C, reference number, N01-HD-9-0011 and the Liver Tissue Cell Distribution System is funded by NIH contract number N01-DK-7-0004/HHSN267200700004C.

scholarly journals Quantification of aggrecan and link-protein mRNA in human articular cartilage of different ages by competitive reverse transcriptase-PCR

1996 ◽  
Vol 319 (2) ◽  
pp. 489-498 ◽  
Author(s):  
Mark C BOLTON ◽  
Jayesh DUDHIA ◽  
Michael T BAYLISS
Keyword(s):  
Articular Cartilage ◽  
Reverse Transcriptase ◽  
Mrna Expression ◽  
Northern Hybridization ◽  
Human Articular Cartilage ◽  
Rt Pcr ◽  
Link Protein ◽  
Reverse Transcriptase Pcr ◽  
Age Related ◽  
Age Related Changes

A competitive reverse transcriptase–PCR (RT-PCR) assay has been developed for the quantification of particular mRNA species in human articular cartilage. Competitor RNA species were synthesized that differed from the amplified target sequence only by the central insertion of an EcoRI restriction site. By using known amounts of synthetic target and competitor RNA, it was shown that competitor RNA molecules designed in this way are reverse-transcribed and amplified with equal efficiency to the target of interest. Furthermore quantification could be performed during the plateau phase of the PCR, which was necessary when using ethidium bromide fluorescence as a detection system. The inhibition of aggrecan and link-protein mRNA expression by interleukin 1 or tumour necrosis factor in monolayers of human articular chondrocytes quantified by this competitive RT-PCR method compared favourably with Northern hybridization studies. The main advantage of this technique is that it can be used to quantify levels of mRNA with RNA extracted directly from 100 mg wet weight of human articular cartilage. Age-related changes in aggrecan and link-protein mRNA were therefore quantified in human articular cartilage directly after dissection from the joint. The concentration of link-protein mRNA was higher in immature cartilage than in mature cartilage when expressed relative to the amount of glyceraldehyde-3-phosphate dehydrogenase mRNA, but no age-related changes were observed in aggrecan mRNA expression. The ratio of aggrecan to link-protein mRNA was higher in mature cartilage than in immature tissue. These age-related differences in the molecular stoichiometry of aggrecan and link-protein mRNA might have implications with respect to the regulation of the formation and the stability of the proteoglycan aggregates in cartilage.

Age-related Changes In Pax7 mRNA Expression In The Rat Activated Diaphragm

2011 ◽  
Vol 43 (Suppl 1) ◽  
pp. 416-417
Author(s):  
Minako Kawai ◽  
Kiyokazu Saitsu ◽  
Hiroki Yamashita ◽  
Maiko Tanaka ◽  
Hirofumi Miyata
Keyword(s):  
Mrna Expression ◽  
Age Related ◽  
Age Related Changes

scholarly journals Age-related changes in the synthesis and mRNA expression of decorin and aggrecan in human meniscus and articular cartilage

2001 ◽  
Vol 9 (1) ◽  
pp. 33-41 ◽  
Author(s):  
A. McAlinden ◽  
J. Dudhia ◽  
M.C. Bolton ◽  
P. Lorenzo ◽  
D. Heinegård ◽  
...  
Keyword(s):  
Articular Cartilage ◽  
Mrna Expression ◽  
Age Related ◽  
Age Related Changes
Sign in / Sign up

Export Citation Format

Share Document