scholarly journals Age-related changes in the synthesis and mRNA expression of decorin and aggrecan in human meniscus and articular cartilage

2001 ◽  
Vol 9 (1) ◽  
pp. 33-41 ◽  
Author(s):  
A. McAlinden ◽  
J. Dudhia ◽  
M.C. Bolton ◽  
P. Lorenzo ◽  
D. Heinegård ◽  
...  
Keyword(s):  
Articular Cartilage ◽  
Mrna Expression ◽  
Age Related ◽  
Age Related Changes

scholarly journals Quantification of aggrecan and link-protein mRNA in human articular cartilage of different ages by competitive reverse transcriptase-PCR

1996 ◽  
Vol 319 (2) ◽  
pp. 489-498 ◽  
Author(s):  
Mark C BOLTON ◽  
Jayesh DUDHIA ◽  
Michael T BAYLISS
Keyword(s):  
Articular Cartilage ◽  
Reverse Transcriptase ◽  
Mrna Expression ◽  
Northern Hybridization ◽  
Human Articular Cartilage ◽  
Rt Pcr ◽  
Link Protein ◽  
Reverse Transcriptase Pcr ◽  
Age Related ◽  
Age Related Changes

A competitive reverse transcriptase–PCR (RT-PCR) assay has been developed for the quantification of particular mRNA species in human articular cartilage. Competitor RNA species were synthesized that differed from the amplified target sequence only by the central insertion of an EcoRI restriction site. By using known amounts of synthetic target and competitor RNA, it was shown that competitor RNA molecules designed in this way are reverse-transcribed and amplified with equal efficiency to the target of interest. Furthermore quantification could be performed during the plateau phase of the PCR, which was necessary when using ethidium bromide fluorescence as a detection system. The inhibition of aggrecan and link-protein mRNA expression by interleukin 1 or tumour necrosis factor in monolayers of human articular chondrocytes quantified by this competitive RT-PCR method compared favourably with Northern hybridization studies. The main advantage of this technique is that it can be used to quantify levels of mRNA with RNA extracted directly from 100 mg wet weight of human articular cartilage. Age-related changes in aggrecan and link-protein mRNA were therefore quantified in human articular cartilage directly after dissection from the joint. The concentration of link-protein mRNA was higher in immature cartilage than in mature cartilage when expressed relative to the amount of glyceraldehyde-3-phosphate dehydrogenase mRNA, but no age-related changes were observed in aggrecan mRNA expression. The ratio of aggrecan to link-protein mRNA was higher in mature cartilage than in immature tissue. These age-related differences in the molecular stoichiometry of aggrecan and link-protein mRNA might have implications with respect to the regulation of the formation and the stability of the proteoglycan aggregates in cartilage.

scholarly journals Oxidative changes and signalling pathways are pivotal in initiating age-related changes in articular cartilage

2014 ◽  
Vol 75 (2) ◽  
pp. 449-458 ◽  
Author(s):  
Wang Hui ◽  
David A Young ◽  
Andrew D Rowan ◽  
Xin Xu ◽  
Tim E Cawston ◽  
...  
Keyword(s):  
Articular Cartilage ◽  
Signalling Pathways ◽  
Age Related ◽  
Age Related Changes

Age-related changes in D1 and D2 receptor mRNA expression in postmortem human putamen with and without multiple small infarcts

1998 ◽  
Vol 243 (1-3) ◽  
pp. 37-40 ◽  
Author(s):  
Hideo Tohgi ◽  
Kimiaki Utsugisawa ◽  
Masahiro Yoshimura ◽  
Yuriko Nagane ◽  
Masatoshi Mihara
Keyword(s):  
Mrna Expression ◽  
D2 Receptor ◽  
Receptor Mrna ◽  
Age Related ◽  
Age Related Changes

Age-Related Changes in the Synthesis of Matrix Macromolecules by Bovine Articular Cartilage

1989 ◽  
Vol 19 (2-4) ◽  
pp. 121-133 ◽  
Author(s):  
Philippe Front ◽  
France Aprile ◽  
Dragoslav R. Mitrovic ◽  
David A. Swann
Keyword(s):  
Articular Cartilage ◽  
Bovine Articular Cartilage ◽  
Age Related ◽  
Age Related Changes

CHANGES IN SWINE KNEE ARTICULAR CARTILAGE DURING GROWTH

1979 ◽  
Vol 59 (1) ◽  
pp. 167-179 ◽  
Author(s):  
T. NAKANO ◽  
F. X. AHERNE ◽  
J. R. THOMPSON
Keyword(s):  
Hyaluronic Acid ◽  
Articular Cartilage ◽  
Dry Matter ◽  
Endochondral Ossification ◽  
Keratan Sulfate ◽  
Cartilage Thickness ◽  
Cell Necrosis ◽  
Normal Cartilage ◽  
Age Related ◽  
Age Related Changes

Twenty-five crossbred boars reared under normal conditions were serially slaughtered at the age of 3 days, and 5, 10, 20 and 30 wk. Five boars were slaughtered at each age and morphological, histochemical and biochemical age-related changes in femoral condylar articular cartilage were studied. No osteochondrotic joints were found in pigs 10 wk of age or younger, while 7 of the 10 boars slaughtered at 20 and 30 wk of age were osteochondrotic. Cartilage thickness increased (P < 0.05) until the age of 5 wk and decreased (P < 0.05) thereafter. Cell density decreased (P < 0.05) as age advanced. Age-associated changes found in the chemical composition of the cartilage were an increase in the concentration of dry matter and hydroxyproline and a decrease in the concentration of glycosaminoglycans (GAG) including chondroitin sulfate (ChS), keratan sulfate and hyaluronic acid. The proportions of soluble proteoglycan and 4-sulfated disaccharide from the ChS fraction decreased (P < 0.05) while the proportion of 6-sulfated disaccharide from ChS increased (P < 0.05). Osteochondrosis was observed as a disturbed endochondral ossification, and softening and fracture of the cartilage. The former was accompanied by a loss of intercellular GAG and cell necrosis, and the latter by local losses of GAG and cells. Osteochondrotic cartilage also contained higher proportions of soluble proteoglycan and 6-sulfated disaccharide, and lower proportions of 4-sulfated disaccharide than did the visually normal cartilage.

Hyaluronate in articular cartilage: Age-related changes

1978 ◽  
Vol 26 (1) ◽  
pp. 19-21 ◽  
Author(s):  
E. J. -M. A. Thonar ◽  
M. B. E. Sweet ◽  
A. R. Immelman ◽  
G. Lyons
Keyword(s):  
Articular Cartilage ◽  
Age Related ◽  
Age Related Changes

Age-related Changes in the Antigenicity of Human Articular Cartilage Proteoglycans

1982 ◽  
Vol 2 (1) ◽  
pp. 45-60 ◽  
Author(s):  
Brian R. Champion ◽  
Agnes Reiner ◽  
Peter J. Roughley ◽  
A. Robin Poole
Keyword(s):  
Articular Cartilage ◽  
Human Articular Cartilage ◽  
Age Related ◽  
Cartilage Proteoglycans ◽  
Age Related Changes
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