Maternal Perinatal Undernutrition Alters Postnatal Development of Chromaffin Cells in the Male Rat Adrenal Medulla

2009 ◽  
Vol 90 (1) ◽  
pp. 54-66 ◽  
Author(s):  
Olivier Molendi-Coste ◽  
Christine Laborie ◽  
Maria Cristina Scarpa ◽  
Valérie Montel ◽  
Didier Vieau ◽  
...  
Keyword(s):  
Postnatal Development ◽  
Adrenal Medulla ◽  
Chromaffin Cells ◽  
Male Rat

scholarly journals Asthma Pregnancy Alters Postnatal Development of Chromaffin Cells in the Rat Adrenal Medulla

PLoS ONE ◽  
2011 ◽  
Vol 6 (5) ◽  
pp. e20337 ◽  
Author(s):  
Xiu-Ming Wu ◽  
Cheng-Ping Hu ◽  
Xiao-Zhao Li ◽  
Ye-Qiang Zou ◽  
Jun-Tao Zou ◽  
...  
Keyword(s):  
Postnatal Development ◽  
Adrenal Medulla ◽  
Chromaffin Cells

Immunogold labeling of the calcium binding protein synexin in isolated adrenal chromaffin granules and chromaffin cells

1990 ◽  
Vol 48 (3) ◽  
pp. 952-953
Author(s):  
Gemma A.J. Kuijpers ◽  
Harvey B. Pollard
Keyword(s):  
Adrenal Medulla ◽  
Calcium Binding ◽  
Chromaffin Cells ◽  
Cell Activation ◽  
Structural Information ◽  
Dependent Manner ◽  
Chromaffin Granules ◽  
Immuno Electron Microscopy ◽  
Ultrathin Sections ◽  
Adrenal Chromaffin

Exocytotic fusion of granules in the adrenal medulla chromaffin cell is triggered by a rise in the concentration of cytosolic Ca2+ upon cell activation. The protein synexin, annexin VII, was originally found in the adrenal medulla and has been shown to cause aggregation and to support fusion of chromaffin granules in a Ca2+-dependent manner. We have previously suggested that synexin may there fore play a role in the exocytotic fusion process. In order to obtain more structural information on synexin, we performed immuno-electron microscopy on frozen ultrathin sections of both isolated chromaffin granules and chromaffin cells.Chromaffin granules were isolated from bovine adrenal medulla, and synexin was isolated from bovine lung. Granules were incubated in the presence or absence of synexin (24 μg per mg granule protein) and Ca2+ (1 mM), which induces maximal granule aggregation, in 0.3M sucrose-40m MMES buffer(pH 6.0). Granules were pelleted, washed twice in buffer without synexin and fixed with 2% glutaraldehyde- 2% para formaldehyde in 0.1 M phosphate buffer (GA/PFA) for 30 min. Chromaffin cells were isolated and cultured for 3-5 days, and washed and incubated in Krebs solution with or without 20 uM nicotine. Cells were fixed 90 sec after on set of stimulation with GA/PFA for 30 min. Fixed granule or cell pellets were washed, infiltrated with 2.3 M sucrose in PBS, mounted and frozen in liquid N2.

scholarly journals Multipotent peripheral glial cells generate neuroendocrine cells of the adrenal medulla

Science ◽  
2017 ◽  
Vol 357 (6346) ◽  
pp. eaal3753 ◽  
Author(s):  
Alessandro Furlan ◽  
Vyacheslav Dyachuk ◽  
Maria Eleni Kastriti ◽  
Laura Calvo-Enrique ◽  
Hind Abdo ◽  
...  
Keyword(s):  
Adrenal Medulla ◽  
Chromaffin Cells ◽  
Gene Networks ◽  
System Development ◽  
Motor Nerve ◽  
Neuroendocrine Cells ◽  
Molecular Logic ◽  
Large Numbers ◽  
Cell Niche ◽  
Type Specification

Adrenaline is a fundamental circulating hormone for bodily responses to internal and external stressors. Chromaffin cells of the adrenal medulla (AM) represent the main neuroendocrine adrenergic component and are believed to differentiate from neural crest cells. We demonstrate that large numbers of chromaffin cells arise from peripheral glial stem cells, termed Schwann cell precursors (SCPs). SCPs migrate along the visceral motor nerve to the vicinity of the forming adrenal gland, where they detach from the nerve and form postsynaptic neuroendocrine chromaffin cells. An intricate molecular logic drives two sequential phases of gene expression, one unique for a distinct transient cellular state and another for cell type specification. Subsequently, these programs down-regulate SCP-gene and up-regulate chromaffin cell–gene networks. The AM forms through limited cell expansion and requires the recruitment of numerous SCPs. Thus, peripheral nerves serve as a stem cell niche for neuroendocrine system development.

Interactions Between Bovine Adrenal Medulla Endothelial and Chromaffin Cells

1998 ◽  
pp. 91-107 ◽  
Author(s):  
Fernando F. Vargas ◽  
Soledad Calvo ◽  
Raul Vinet ◽  
Eduardo Rojas
Keyword(s):  
Adrenal Medulla ◽  
Chromaffin Cells ◽  
Bovine Adrenal Medulla

scholarly journals Connexin 43 expression in the testes during postnatal development of finasteride-treated male rat offspring

2018 ◽  
Vol 14 (6) ◽  
pp. 1471-1479 ◽  
Author(s):  
Agnieszka Kolasa-Wołosiuk ◽  
Kamila Misiakiewicz-Has ◽  
Irena Baranowska-Bosiacka ◽  
Izabela Gutowska ◽  
Maciej Tarnowski ◽  
...  
Keyword(s):  
Postnatal Development ◽  
Connexin 43 ◽  
Male Rat ◽  
Treated Male ◽  
Rat Offspring
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