Normal Human Red Cells Treated in vitro with Cephalothin

1971 ◽  
Vol 45 (4) ◽  
pp. 245-249 ◽  
Author(s):  
S. Ferrone ◽  
G. Lambertenghi-Deliliers ◽  
T. Ranzi
Keyword(s):  
Red Cells ◽  
Normal Human ◽  
Human Red Cells

scholarly journals The Action of Two Sulfhydryl Compounds on Normal Human Red Cells

Blood ◽  
1965 ◽  
Vol 25 (4) ◽  
pp. 502-510 ◽  
Author(s):  
GIROLAMO SIRCHIA ◽  
SOLDANO FERRONE ◽  
FRANCESCO MERCURIALI
Keyword(s):  
Mechanism Of Action ◽  
Paroxysmal Nocturnal Hemoglobinuria ◽  
Red Cells ◽  
Experimental Conditions ◽  
Sh Groups ◽  
Normal Human ◽  
Sulfhydryl Compounds ◽  
Human Red Cells

Abstract Treatment of normal human red cells with AET and cysteine, under suitable experimental conditions, modifies them in such a way that their behavior in in vitro hemolysis tests becomes similar to that of the erythrocytes of paroxysmal nocturnal hemoglobinuria. It is felt that alteration of the red cells is due to the -SH groups possessed by both substances. A possible mechanism of action is hypothesized.

Increased Potassium Permeability Induced In Vitro by Menadione in Normal Human Red Cells

2009 ◽  
Vol 18 (1) ◽  
pp. 61-66 ◽  
Author(s):  
Luciano Vettore ◽  
Maria Concetta Matteis ◽  
Gian Carlo Falezza
Keyword(s):  
Red Cells ◽  
Potassium Permeability ◽  
Normal Human ◽  
Human Red Cells

scholarly journals Methemoglobin reduction in red cells: effect of a high oxygen affinity hemoglobin

Blood ◽  
1980 ◽  
Vol 55 (1) ◽  
pp. 116-118
Author(s):  
F Taketa ◽  
KJ Matteson ◽  
JY Chen ◽  
JA Libnoch
Keyword(s):  
Oxygen Affinity ◽  
High Oxygen ◽  
Red Cells ◽  
Combined Effects ◽  
In Vitro System ◽  
Normal Human ◽  
High Oxygen Affinity ◽  
Heterozygous Carriers ◽  
Human Red Cells

Erythrocytes from heterozygous carriers of the high oxygen affinity mutant hemoglobin, Hb Wood, demonstrate lower rates of methemoglobin reduction than normal human red cells when incubated in the in vitro system of Beutler and Baluda. The rate of methemoglobin reduction in red cells from an individual who is heterozygous for both NADH- methoglobin reductase deficiency and Hb Wood shows the combined effects of the two mutations.

scholarly journals Methemoglobin reduction in red cells: effect of a high oxygen affinity hemoglobin

Blood ◽  
1980 ◽  
Vol 55 (1) ◽  
pp. 116-118 ◽  
Author(s):  
F Taketa ◽  
KJ Matteson ◽  
JY Chen ◽  
JA Libnoch
Keyword(s):  
Oxygen Affinity ◽  
High Oxygen ◽  
Red Cells ◽  
Combined Effects ◽  
In Vitro System ◽  
Normal Human ◽  
High Oxygen Affinity ◽  
Heterozygous Carriers ◽  
Human Red Cells

Abstract Erythrocytes from heterozygous carriers of the high oxygen affinity mutant hemoglobin, Hb Wood, demonstrate lower rates of methemoglobin reduction than normal human red cells when incubated in the in vitro system of Beutler and Baluda. The rate of methemoglobin reduction in red cells from an individual who is heterozygous for both NADH- methoglobin reductase deficiency and Hb Wood shows the combined effects of the two mutations.

In Vitro Assay of Platelet Adhesiveness with Washed and Tanned Human Red Cells

1968 ◽  
Vol 20 (03/04) ◽  
pp. 384-396 ◽  
Author(s):  
G Zbinden ◽  
S Tomlin
Keyword(s):  
Platelet Adhesion ◽  
Sodium Citrate ◽  
Blood Platelets ◽  
In Vitro Assay ◽  
Red Cells ◽  
Platelet Adhesiveness ◽  
Vitro Assay ◽  
In Vitro System ◽  
Human Red Cells

SummaryAn in vitro system is described in which adhesion of blood platelets to washed and tannic acid-treated red cells was assayed quantitatively by microscopic observation. ADP, epinephrine and TAME produced a reversible increase in platelet adhesiveness which was antagonized by AMP. With Evans blue, polyanetholsulfonate, phthalanilide NSC 38280, thrombin and heparin at concentrations above 1-4 u/ml the increase was irreversible. The ADP-induced increase in adhesiveness was inhibited by sodium citrate, EDTA, AMP, ATP and N-ethylmaleimide. EDTA, AMP and the SH-blocker N-ethylmaleimide also reduced spontaneous platelet adhesion to red cells. No significant effects were observed with adenosine, phenprocoumon, 5-HT, phthalanilide NSC 57155, various estrogens, progestogens and fatty acids, acetylsalicylic acid and similarly acting agents, hydroxylamine, glucose and KCN. The method may be useful for the screening of thrombogenic and antithrombotic properties of drugs.

scholarly journals Some Interrelationships of Blood and the Fava Bean Principle in Vitro

Blood ◽  
1952 ◽  
Vol 7 (7) ◽  
pp. 721-728 ◽  
Author(s):  
WILLIAM P. CREGER ◽  
HOUGHTON GIFFORD
Keyword(s):  
Animal Species ◽  
Gamma Globulin ◽  
Test Tube ◽  
Red Cells ◽  
Red Cell ◽  
Fava Bean ◽  
Increased Susceptibility ◽  
Human Red Cells

Abstract 1. Saline suspensions of human red cells, as well as those of several animal species, were agglutinated by normal saline extracts of the Fava bean. 2. This agglutination was potentiated in titer 100-fold in a medium of 10 per cent acacia, as a diluent. 3. The inhibition of the hemagglutination action of the Fava bean extract by human serum was apparently attributable to the gamma globulin fraction. 4. The Fava bean principle could be transferred from cell to cell, as shown by heat-elution and acacia technics. 5. Fava-sensitized red cells did not exhibit increased susceptibility in the test tube to complement, hemolysin, or osmotic or mechanical fragility. 6. The mechanism of in vivo red cell destruction in Favism is as yet unknown, but a special immunologic susceptibility to the action of the bean’s principle is suspected in certain persons. 7. It is suggested that the relation of acacia to Fava-sensitized red cells may form the basis of a diagnostic test for Favism in the early, acute stages of the disease.

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