Influence of Cell Density on the Acridine Orange Binding to Deoxyribonucleoprotein Complex in Leucocytes from Patients with Infectious Mononucleosis and Acute Leukaemia

1972 ◽  
Vol 48 (4) ◽  
pp. 227-233 ◽  
Author(s):  
L. Bolund ◽  
L.E. de Bault ◽  
G.E. Foley ◽  
G. Gahrton ◽  
D. Killander ◽  
...  
Keyword(s):  
Acridine Orange ◽  
Cell Density ◽  
Infectious Mononucleosis ◽  
Acute Leukaemia ◽  
Deoxyribonucleoprotein Complex

scholarly journals Structural Changes in the Deoxyribonucleoprotein (DNP) Complex of Leukocytes from Patients with Infectious Mononucleosis

Blood ◽  
1970 ◽  
Vol 35 (3) ◽  
pp. 322-332 ◽  
Author(s):  
L. BOLUND ◽  
G. GAHRTON ◽  
D. KILLANDER ◽  
R. RIGLER ◽  
BRITTA WAHREN
Keyword(s):  
Acridine Orange ◽  
Acute Phase ◽  
Infectious Mononucleosis ◽  
Binding Sites ◽  
Early Phase ◽  
Structural Changes ◽  
Number Of Binding Sites ◽  
Healthy Persons

Abstract Quantitative microfluorimetric measurements on individual acridine orange (AO) stained cells showed that leukocytes from patients with infectious mononucleosis (IM) exhibited considerably greater AO binding to deoxyribonucleoprotein (DNP) than leukocytes from healthy persons. The higher binding of AO could not be explained by an increase in the amount of DNP in IM cells but by an increased number of binding sites in DNP accessible to AO. This was the result of structural changes in the DNP complex of the IM cells, possibly due to weakened bonds between the DNA and protein moieties of DNP. The binding of AO to IM leukocytes was highest in the early phase of the disease and usually normalized after recovery. However, an increased AO binding of leukocytes from patients after recovery was frequently observed when these cells were exposed to plasma from patients in the acute phase of IM.

DNA Synthesis in Infectious Mononucleosis and Acute Leukaemia

1963 ◽  
Vol 29 (5) ◽  
pp. 257-266 ◽  
Author(s):  
A.J. Hale ◽  
E.H. Cooper
Keyword(s):  
Dna Synthesis ◽  
Infectious Mononucleosis ◽  
Acute Leukaemia

Vacuoles Induced in Mammalian Cells by Helicobacter Cytotoxin are Acidic Organelles

1990 ◽  
Vol 48 (3) ◽  
pp. 168-169
Author(s):  
M. H. Chestnut ◽  
C. E. Catrenich
Keyword(s):  
Acridine Orange ◽  
Mammalian Cells ◽  
Laser Scanning ◽  
Laser Scanning Microscopy ◽  
Confocal Laser ◽  
Ulcer Disease ◽  
Gastroduodenal Disease ◽  
H Pylori

Helicobacter pylori is a non-invasive, Gram-negative spiral bacterium first identified in 1983, and subsequently implicated in the pathogenesis of gastroduodenal disease including gastritis and peptic ulcer disease. Cytotoxic activity, manifested by intracytoplasmic vacuolation of mammalian cells in vitro, was identified in 55% of H. pylori strains examined. The vacuoles increase in number and size during extended incubation, resulting in vacuolar and cellular degeneration after 24 h to 48 h. Vacuolation of gastric epithelial cells is also observed in vivo during infection by H. pylori. A high molecular weight, heat labile protein is believed to be responsible for vacuolation and to significantly contribute to the development of gastroduodenal disease in humans. The mechanism by which the cytotoxin exerts its effect is unknown, as is the intracellular origin of the vacuolar membrane and contents. Acridine orange is a membrane-permeant weak base that initially accumulates in low-pH compartments. We have used acridine orange accumulation in conjunction with confocal laser scanning microscopy of toxin-treated cells to begin probing the nature and origin of these vacuoles.

Increassed parietal cell density in patients with perfurated duodenal ulcer: Comparison with non-complicated duodenal ulcer patients

2001 ◽  
Vol 120 (5) ◽  
pp. A250-A250
Author(s):  
A BARBOSA ◽  
C MENDES ◽  
L COELHO ◽  
C RODRIGUES ◽  
M MACHADO ◽  
...  
Keyword(s):  
Duodenal Ulcer ◽  
Cell Density ◽  
Parietal Cell

Therapeutic role of cell kinetics in acute leukaemia

1978 ◽  
Vol 7 (2) ◽  
pp. 339-362 ◽  
Author(s):  
Zalmen A. Arlin ◽  
Jerrold Fried ◽  
Bayard D. Clarkson
Keyword(s):  
Acute Leukaemia ◽  
Cell Kinetics ◽  
Therapeutic Role
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