Acid Phosphatase Activity in Normal Human Blood and Bone Marrow Cells as Demonstrated by the Azo Dye Method

1963 ◽  
Vol 30 (5) ◽  
pp. 310-316 ◽  
Author(s):  
L. Rozenszajn ◽  
G. Marshak ◽  
P. Efrati
Keyword(s):  
Bone Marrow ◽  
Acid Phosphatase ◽  
Phosphatase Activity ◽  
Human Blood ◽  
Acid Phosphatase Activity ◽  
Azo Dye ◽  
Bone Marrow Cells ◽  
Normal Human ◽  
Normal Human Blood ◽  
Marrow Cells

scholarly journals A CYTOCHEMICAL EVALUATION OF PYROANTIMONATE BINDING TO THE PLASMALEMMA OF BLOOD AND BONE MARROW CELLS AND ITS RELATIONSHIP TO CELLULAR MATURATION

1972 ◽  
Vol 20 (11) ◽  
pp. 880-895 ◽  
Author(s):  
G. ADOLPH ACKERMAN ◽  
MICHAEL A. CLARK
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Cells ◽  
Ruthenium Red ◽  
Binding Ability ◽  
Neutrophilic Leukocytes ◽  
Different Types ◽  
Normal Human ◽  
Normal Human Blood ◽  
Membrane Reactivity ◽  
Marrow Cells

Normal human blood and bone marrow cells exposed to the pyroantimonate reaction exhibit a selective binding of pyroantimonate to sites associated with the plasmalemma of neutrophilic leukocytes, erythrocytes and certain of their precursors. Chemical distinctions exist between the plasmalemma of different types of blood and bone marrow cells, and major changes in the cationic binding ability of neutrophilic and erythrocytic cells can be correlated with different phases in their differentiation. Plasmalemmal pyroantimonate-osmium-reactive cation is bound with glycoprotein in the erythrocytic elements and to both phospholipid and glycoprotein moieties in neutrophilic cells. The cellular distribution, membrane localization and degree of membrane reactivity obtained with the pyroantimonate-osmium reaction is distinct from that obtained with Thorotrast and ruthenium red.

scholarly journals Differential effects of nitric oxide on erythroid and myeloid colony growth from CD34+ human bone marrow cells

Blood ◽  
1996 ◽  
Vol 87 (3) ◽  
pp. 977-982 ◽  
Author(s):  
PJ Shami ◽  
JB Weinberg
Keyword(s):  
Bone Marrow ◽  
Mononuclear Cells ◽  
Bone Marrow Cells ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Monocytic Differentiation ◽  
Colony Forming Unit ◽  
Normal Human ◽  
Normal Human Bone Marrow ◽  
Marrow Cells

Nitric oxide (NO) is a reactive molecule with numerous physiologic and pathophysiologic roles affecting the nervous, cardiovascular, and immune systems. In previous work, we have demonstrated that NO inhibits the growth and induces the monocytic differentiation of cells of the HL- 60 cell line. We have also demonstrated that NO inhibits the growth of acute nonlymphocytic leukemia cells freshly isolated from untreated patients and increases monocytic differentiation antigens in some. In the present work, we studied the effect of NO on the growth and differentiation of normal human bone marrow cells in vitro. Mononuclear cells isolated from human bone marrow were cultured in semisolid media and treated with the NO-donating agents sodium nitroprusside (SNP) or S- nitroso-acetyl penicillamine (SNAP) (0.25 to 1 mmol/L). Both agents decreased colony-forming unit-erythroid (CFU-E) and colony-forming unit- granulocyte macrophage (CFU-GM) formation by 34% to 100%. When CD34+ cells were examined, we noted that these cells responded to SNP and SNAP differently than did the mononuclear cells. At a concentration range of 0.25 to 1 mmol/L, SNP inhibited the growth of CFU-E by 30% to 75%. However, at the same concentration range, SNP increased the number of CFU-GM by up to 94%. At concentrations of 0.25 to 1 mmol/L, SNAP inhibited the growth of CFU-E by 33% to 100%. At a concentration of 0.25 mmol/L, SNAP did not affect CFU-GM. At higher concentrations, SNAP inhibited the growth of CFU-GM. Although SNP increased intracellular levels of cGMP in bone marrow cells, increasing cGMP in cells by addition of 8-Br-cGMP (a membrane permeable cGMP analogue) did not reproduce the observed NO effects on bone marrow colonies. These results demonstrate that NO can influence the growth and differentiation of normal human bone marrow cells. NO (generated in the bone marrow microenvironment) may play an important role modulating the growth and differentiation of bone marrow cells in vivo.

scholarly journals THE DISTRIBUTION OF HISTOCHEMICALLY DEMONSTRABLE GLYCOGEN IN HUMAN BLOOD AND BONE MARROW CELLS

Blood ◽  
1949 ◽  
Vol 4 (1) ◽  
pp. 54-59 ◽  
Author(s):  
MAX WACHSTEIN
Keyword(s):  
Bone Marrow ◽  
Human Blood ◽  
Acid Treatment ◽  
Bone Marrow Cells ◽  
Periodic Acid ◽  
Myeloid Cells ◽  
Staining Reaction ◽  
Cytoplasmic Staining ◽  
Chemical Association ◽  
Marrow Cells

Abstract By applying Schiff’s reagent after periodic acid treatment to blood and bone marrow films, a cytoplasmic staining reaction is seen in some cells of the myeloid series, as well as in megakaryocytes and platelets. The intensity of the staining reaction in the myeloid cells increases with their maturation. The staining reaction can be prevented altogether in alcohol-fixed films by salivary digestion, but only incompletely in air-dried films. The staining reaction is due to the presence of glycogen in some chemical association, possibly with protein.

The rate of sister chromatid exchange in normal human bone marrow cells

1979 ◽  
Vol 50 (2) ◽  
pp. 213-216 ◽  
Author(s):  
R. Becher ◽  
C. G. Schmidt ◽  
Gabriele Theis ◽  
D. K. Hossfeld
Keyword(s):  
Bone Marrow ◽  
Sister Chromatid ◽  
Sister Chromatid Exchange ◽  
Bone Marrow Cells ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Normal Human ◽  
Normal Human Bone Marrow ◽  
Marrow Cells

Acid Phosphatase Activity in Human Blood Cells

Nature ◽  
1962 ◽  
Vol 195 (4838) ◽  
pp. 297-297 ◽  
Author(s):  
ARTHUR F. GOLDBERG ◽  
TIBOR BARKA
Keyword(s):  
Acid Phosphatase ◽  
Phosphatase Activity ◽  
Human Blood ◽  
Blood Cells ◽  
Acid Phosphatase Activity ◽  
Human Blood Cells

scholarly journals HISTOCHEMICAL DEMONSTRATION OF ACID PHOSPHATASE ACTIVITY IN OSTEOCLASTS

1959 ◽  
Vol 7 (1) ◽  
pp. 39-41 ◽  
Author(s):  
M. S. BURSTONE
Keyword(s):  
Alkaline Phosphatase ◽  
Acid Phosphatase ◽  
Phosphatase Activity ◽  
Comparative Studies ◽  
Acid Phosphatase Activity ◽  
Azo Dye ◽  
Histochemical Demonstration ◽  
Accurate Localization ◽  
Acid And Alkaline Phosphatase ◽  
Cold Acetone

High acid phosphatase activity was observed in osteoclasts of several species using a reproducible azo-dye technique. High activity of two distinct enzymes, acid and alkaline phosphatase, are associated with osteoclasts and osteoblasts respectivey. Althouth frozen-dried tissues are recommended for definitive studies, the enzyme techniques used give satisfactory results with cold acetone-fixed tissues. The most accurate localization of acid phosphatase in osteoclasts in controlled comparative studies is obtained with double-embedded frozen-dried undecalcified tissues in conjunction with naphthol AS-phosphates.

Sign in / Sign up

Export Citation Format

Share Document