The rate of sister chromatid exchange in normal human bone marrow cells

1979 ◽  
Vol 50 (2) ◽  
pp. 213-216 ◽  
Author(s):  
R. Becher ◽  
C. G. Schmidt ◽  
Gabriele Theis ◽  
D. K. Hossfeld
Keyword(s):  
Bone Marrow ◽  
Sister Chromatid ◽  
Sister Chromatid Exchange ◽  
Bone Marrow Cells ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Normal Human ◽  
Normal Human Bone Marrow ◽  
Marrow Cells

scholarly journals Differential effects of nitric oxide on erythroid and myeloid colony growth from CD34+ human bone marrow cells

Blood ◽  
1996 ◽  
Vol 87 (3) ◽  
pp. 977-982 ◽  
Author(s):  
PJ Shami ◽  
JB Weinberg
Keyword(s):  
Bone Marrow ◽  
Mononuclear Cells ◽  
Bone Marrow Cells ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Monocytic Differentiation ◽  
Colony Forming Unit ◽  
Normal Human ◽  
Normal Human Bone Marrow ◽  
Marrow Cells

Nitric oxide (NO) is a reactive molecule with numerous physiologic and pathophysiologic roles affecting the nervous, cardiovascular, and immune systems. In previous work, we have demonstrated that NO inhibits the growth and induces the monocytic differentiation of cells of the HL- 60 cell line. We have also demonstrated that NO inhibits the growth of acute nonlymphocytic leukemia cells freshly isolated from untreated patients and increases monocytic differentiation antigens in some. In the present work, we studied the effect of NO on the growth and differentiation of normal human bone marrow cells in vitro. Mononuclear cells isolated from human bone marrow were cultured in semisolid media and treated with the NO-donating agents sodium nitroprusside (SNP) or S- nitroso-acetyl penicillamine (SNAP) (0.25 to 1 mmol/L). Both agents decreased colony-forming unit-erythroid (CFU-E) and colony-forming unit- granulocyte macrophage (CFU-GM) formation by 34% to 100%. When CD34+ cells were examined, we noted that these cells responded to SNP and SNAP differently than did the mononuclear cells. At a concentration range of 0.25 to 1 mmol/L, SNP inhibited the growth of CFU-E by 30% to 75%. However, at the same concentration range, SNP increased the number of CFU-GM by up to 94%. At concentrations of 0.25 to 1 mmol/L, SNAP inhibited the growth of CFU-E by 33% to 100%. At a concentration of 0.25 mmol/L, SNAP did not affect CFU-GM. At higher concentrations, SNAP inhibited the growth of CFU-GM. Although SNP increased intracellular levels of cGMP in bone marrow cells, increasing cGMP in cells by addition of 8-Br-cGMP (a membrane permeable cGMP analogue) did not reproduce the observed NO effects on bone marrow colonies. These results demonstrate that NO can influence the growth and differentiation of normal human bone marrow cells. NO (generated in the bone marrow microenvironment) may play an important role modulating the growth and differentiation of bone marrow cells in vivo.

scholarly journals Prostate-specific Antigen Expression in Normal Human Bone Marrow Cells

1999 ◽  
Vol 45 (7) ◽  
pp. 1102-1103 ◽  
Author(s):  
Ferdinando Mannello ◽  
Sara Barulli ◽  
Manuela Malatesta ◽  
Stefania Mancini ◽  
Pietro Leoni ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Prostate Specific Antigen ◽  
Bone Marrow Cells ◽  
Specific Antigen ◽  
Antigen Expression ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Normal Human ◽  
Normal Human Bone Marrow ◽  
Marrow Cells

scholarly journals Biosynthesis of granule proteins in normal human bone marrow cells. Gelatinase is a marker of terminal neutrophil differentiation

Blood ◽  
1995 ◽  
Vol 85 (3) ◽  
pp. 812-817 ◽  
Author(s):  
N Borregaard ◽  
M Sehested ◽  
BS Nielsen ◽  
H Sengelov ◽  
L Kjeldsen
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Cells ◽  
Myeloid Cells ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Specific Granules ◽  
Normal Human ◽  
Neutrophil Differentiation ◽  
Normal Human Bone Marrow ◽  
Marrow Cells

Differentiation and maturation of myeloid cells is characterized by the sequential acquisition of two distinct cytoplasmic granule subsets, azurophil granules and specific granules. We recently showed the existence of a third granule subset, gelatinase granules. To investigate whether appearance of gelatinase granules marks a further step in maturation of myeloid cells beyond the appearance of specific granules, we sorted normal human bone marrow cells into one of three groups according to maturity by centrifugation on Percoll density gradients. The biosynthesis of myeloperoxidase (MPO) (an azurophil granule marker), lactoferrin and neutrophil gelatinase-associated lipocalin NGAL (specific granules markers) and gelatinase was then studied in each of these groups. We found that gelatinase was synthesized mainly in the group containing band cells and segmented cells. This contrasted with lactoferrin and NGAL, which were synthesized almost exclusively in the group containing myelocytes and metamyelocytes, and with MPO, which was mainly synthesized in the group containing myeloblasts and promyelocytes. Immunocytochemistry was in full agreement with the biosynthesis data, and showed that gelatinase appears in band cells, whereas NGAL and lactoferrin both appear in myelocytes. Thus, acquisition of gelatinase granules marks a step in neutrophil differentiation beyond the appearance of specific granules.

Expression and characterization of erythropoietin receptors on normal human bone marrow cells

1989 ◽  
Vol 7 (3) ◽  
pp. 156-167 ◽  
Author(s):  
Shigeru Hoshino ◽  
Masanao Teramura ◽  
Masatomo Takahashi ◽  
Toshiko Motoji ◽  
Kazuo Oshimi ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Cells ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Normal Human ◽  
Normal Human Bone Marrow ◽  
Marrow Cells

Interleukin-4 suppresses the interleukin-3 dependent erythroid colony formation from normal human bone marrow cells

1990 ◽  
Vol 74 (3) ◽  
pp. 246-250 ◽  
Author(s):  
Joost Th. M. de Wolf ◽  
John A. M. Beentjes ◽  
Mariet T. Esselink ◽  
Jan W. Smit ◽  
M. Ruud Halie ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Colony Formation ◽  
Bone Marrow Cells ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Interleukin 4 ◽  
Interleukin 3 ◽  
Normal Human ◽  
Normal Human Bone Marrow ◽  
Marrow Cells
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