Proliferative Potentials of Bone Marrow and Blood Cells Studied by in vitro Uptake of H3-Thymidine

1959 ◽  
Vol 21 (1) ◽  
pp. 1-15 ◽  
Author(s):  
V.P. Bond ◽  
T.M. Fliedner ◽  
E.P. Cronkite ◽  
J.R. Rubini ◽  
G. Brecher ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Blood Cells ◽  
In Vitro Uptake

IN VITRO UPTAKE OF Fe59 BY BLOOD CELLS FROM NORMAL BIRDS AND BIRDS WITH ERYTHROBLASTOSIS

1963 ◽  
Vol 41 (1) ◽  
pp. 2057-2064 ◽  
Author(s):  
R. Bather ◽  
Stella Dzuibalo ◽  
C. le Q. Darcel
Keyword(s):  
Bone Marrow ◽  
Ammonium Sulphate ◽  
Blood Cells ◽  
Red Cells ◽  
Normal Blood ◽  
Promoting Effect ◽  
Heme Synthesis ◽  
Heat Resistant ◽  
In Vitro Uptake

The cellular elements of blood obtained from birds with virus-induced erythroblastosis rapidly incorporated radioactivity when incubated in vitro with Fe59Cl3. This effect was not seen until primitive red cells entered the circulating blood. Little of the iron that was incorporated reached heme. Instead most of it was retained by a heat-resistant component which was precipitable with 35% ammonium sulphate and had properties suggestive of a ferritin-like substance. Plasma from leukemic blood had a slight promoting effect on incorporation of Fe59 into normal blood cells in vitro when compared with normal plasma.Blood cells from birds in which the bone marrow was rendered hyperactive with phenylhydrazine also incorporated Fe59 in vitro, a large proportion of this reaching heme. Incorporation into the heme fraction was also observed in blood cells from erythroblastosis-infected birds treated with phenylhydrazine, indicating that heme synthesis was not completely blocked in these birds.

IN VITRO UPTAKE OF Fe59 BY BLOOD CELLS FROM NORMAL BIRDS AND BIRDS WITH ERYTHROBLASTOSIS

1963 ◽  
Vol 41 (10) ◽  
pp. 2057-2064 ◽  
Author(s):  
R. Bather ◽  
Stella Dzuibalo ◽  
C. le Q. Darcel
Keyword(s):  
Bone Marrow ◽  
Ammonium Sulphate ◽  
Blood Cells ◽  
Red Cells ◽  
Normal Blood ◽  
Promoting Effect ◽  
Heme Synthesis ◽  
Heat Resistant ◽  
In Vitro Uptake

The cellular elements of blood obtained from birds with virus-induced erythroblastosis rapidly incorporated radioactivity when incubated in vitro with Fe59Cl3. This effect was not seen until primitive red cells entered the circulating blood. Little of the iron that was incorporated reached heme. Instead most of it was retained by a heat-resistant component which was precipitable with 35% ammonium sulphate and had properties suggestive of a ferritin-like substance. Plasma from leukemic blood had a slight promoting effect on incorporation of Fe59 into normal blood cells in vitro when compared with normal plasma.Blood cells from birds in which the bone marrow was rendered hyperactive with phenylhydrazine also incorporated Fe59 in vitro, a large proportion of this reaching heme. Incorporation into the heme fraction was also observed in blood cells from erythroblastosis-infected birds treated with phenylhydrazine, indicating that heme synthesis was not completely blocked in these birds.

Sex chiniaerism and germ cell distribution in a series of chimaeric mice

Development ◽  
1975 ◽  
Vol 33 (1) ◽  
pp. 205-216
Author(s):  
Anne McLaren
Keyword(s):  
Bone Marrow ◽  
Blood Cells ◽  
Chromosome Analysis ◽  
Chromosome Morphology ◽  
Mixed Population ◽  
Cell Distribution ◽  
Single Sex ◽  
Xx Males

1. Of 30 mice born from aggregation of embryos from a multiple recessive strain with F1 embryos carrying the contrasting alleles, 4 females and 20 males proved to be overtly chimaeric. 2. Three XX/XX females, five XY/XY males and eight XY/XX males were identified by chromosome analysis. Thus 50 % of the population analysed were sex chimaeras, and all of these developed as phenotypic males, though one showed evidence of hermaphroditism. 3. In seven XY/XX chimaeras that bred, the genetic component undergoing spermatogenesis coincided in every case with the component identified by chromosome morphology as XY. 4. The F1 component predominated in metaphase plates derived from cultured blood cells. Comparison with direct preparations from bone marrow suggested selection in favour of F1 cells, either through differential proliferation of stem cells in vivo or differential response to phytohaemagglutinin in vitro. 5. In XY/XX males, the percentage of XX cells detected varied from 1 % to 98 % in blood, and from 0 % to 80 % in bone marrow. 6. Of eight ‘single-sex’ chimaeras progeny-tested (three XX/XX, five XY/XY), only one showed evidence of a mixed population of germ cells. The proportion of the two types of progeny varied significantly from litter to litter, but was unrelated to the age of the male.

Comparison of cytarabine, mitoxantrone, and CNDAC in vitro treatment of AML bone marrow and peripheral blood cells.

2011 ◽  
Vol 29 (15_suppl) ◽  
pp. 6588-6588
Author(s):  
S. Jagan ◽  
L. A. Paganessi ◽  
S. Gezer ◽  
A. Rizman ◽  
D. Rifai ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Peripheral Blood ◽  
Blood Cells ◽  
Peripheral Blood Cells ◽  
In Vitro Treatment

THE IN-VITRO UPTAKE OF 32P BY RED BLOOD-CELLS IN THYROID DISEASE

The Lancet ◽  
1959 ◽  
Vol 274 (7102) ◽  
pp. 537-539 ◽  
Author(s):  
HelenE.A. Farran ◽  
P. Milutinovic ◽  
A.Stuart Mason
Keyword(s):  
Red Blood Cells ◽  
Thyroid Disease ◽  
Blood Cells ◽  
In Vitro Uptake

scholarly journals Evaluation of erythropoiesis in long-term hamster bone marrow suspension cultures: absence of a requirement for adherent monolayer cells

Blood ◽  
1982 ◽  
Vol 60 (4) ◽  
pp. 999-1006
Author(s):  
CE Eastment ◽  
FW Ruscetti
Keyword(s):  
Bone Marrow ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Adherent Cell ◽  
Erythroid Progenitors ◽  
Large Numbers ◽  
Bone Marrow Cultures ◽  
Vitro Model

In long-term hamster bone marrow cultures, proliferation and differentiation of hemopoietic stem cells occurs for several months without need for hydrocortisone or adherent stromal elements, which are requirements for bone marrow growth in all other species studied. Only the most primitive erythroid progenitors (BFU-E) are produced in the cultures. Following treatment of the cells with erythropoietin, these progenitor cells undergo differentiation into mature hemoglobinized red blood cells. Concomitant addition of erythropoietin (Epo) and prostaglandin-E1 (PGE1) results in the production of large numbers of maturing red blood cells. In cultures stimulated with Epo and PGE1, as many as 70% of the cells are benzidine-positive, while Epo alone stimulated as many as 45% of the cells to become erythroid. Epo and PGE1 do not have any apparent deleterious effect on the continuous hemopoiesis occurring in these cultures. Under identical conditions, syngeneic adherent cell cultures do not produce any erythroid elements. The development of mature red blood cells from primitive erythroid precursors occurs in the presence of Epo alone and without any apparent need for adherent stromal elements. These cultures provide a useful in vitro model for dissecting the positive and negative signals that regulate erythropoiesis.

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