Functional Analysis of an Epstein-Barr Virus-Nontransformed Human B Cell Line

1988 ◽  
Vol 80 (3) ◽  
pp. 139-144 ◽  
Author(s):  
Y. Yasuda ◽  
T. Iwasaki ◽  
T. Hamano ◽  
K. Nagai
Keyword(s):  
Functional Analysis ◽  
B Cell ◽  
Cell Line ◽  
Epstein Barr Virus ◽  
Barr Virus ◽  
Epstein Barr ◽  
Human B Cell

Bcl-2 and c-Myc co-operate in the Epstein – Barr virus-immortalized human B-cell line GM607 but do not confer tumorigenicity

2005 ◽  
Vol 46 (4) ◽  
pp. 581-592 ◽  
Author(s):  
Jennifer L Tomlin ◽  
Barbara-Ann Guinn ◽  
Linda Z Penn ◽  
Neil L Berinstein
Keyword(s):  
B Cell ◽  
Cell Line ◽  
Epstein Barr Virus ◽  
Barr Virus ◽  
Epstein Barr ◽  
Human B Cell

scholarly journals A Somatic Knockout of CBF1 in a Human B-Cell Line Reveals that Induction of CD21 and CCR7 by EBNA-2 Is Strictly CBF1 Dependent and that Downregulation of Immunoglobulin M Is Partially CBF1 Independent

2005 ◽  
Vol 79 (14) ◽  
pp. 8784-8792 ◽  
Author(s):  
Sabine Maier ◽  
Maja Santak ◽  
Anja Mantik ◽  
Kristina Grabusic ◽  
Elisabeth Kremmer ◽  
...  
Keyword(s):  
B Cell ◽  
Cell Line ◽  
Epstein Barr Virus ◽  
Target Genes ◽  
Immunoglobulin M ◽  
Virus Growth ◽  
Barr Virus ◽  
Independent Functions ◽  
Epstein Barr ◽  
Human B Cell

ABSTRACT CBF1 is a cellular highly conserved DNA binding factor that is ubiquitously expressed in all tissues and acts as a repressor of cellular genes. In Epstein-Barr virus growth-transformed B-cell lines, CBF1 serves as a central DNA adaptor molecule for several viral proteins, including the viral transactivator Epstein-Barr virus nuclear antigen 2 (EBNA-2). EBNA-2 binds to CBF1 and thereby gains access to regulatory regions of target genes and activates transcription. We have inactivated the CBF1 gene by homologous recombination in the human B-cell line DG75 and characterized changes in cellular gene expression patterns upon loss of CBF1 and activation of EBNA-2. CBF1-negative DG75 cells were viable and proliferated at wild-type rates. Loss of CBF1 was not sufficient to release repression of the previously described EBNA-2 target genes CD21 or CCR7, whereas induction of both target genes by EBNA-2 required CBF1. In contrast, repression of immunoglobulin M by EBNA-2 was mainly CBF1 independent. CBF1-negative DG75 B cells thus provide an excellent tool to dissect CBF1-dependent and -independent functions exerted by the EBNA-2 protein in future studies.

Establishment and characterization of a new Epstein-Barr virus transformed cell line from a human B cell lymphoma

1990 ◽  
Vol 60 (3) ◽  
pp. 206-212
Author(s):  
Ottmar Janssen ◽  
Barbara Heinze ◽  
Claus R. Bartram ◽  
Michael Pawlita ◽  
Thomas Binder ◽  
...  
Keyword(s):  
B Cell ◽  
Cell Line ◽  
Epstein Barr Virus ◽  
Cell Lymphoma ◽  
B Cell Lymphoma ◽  
Barr Virus ◽  
Transformed Cell Line ◽  
Epstein Barr ◽  
Human B Cell

Interleukin 1α acts as an autocrine growth factor for RPMI1788, an Epstein-Barr virus-transformed human B cell line

1988 ◽  
Vol 18 (7) ◽  
pp. 1027-1031 ◽  
Author(s):  
Peter Vandenabeele ◽  
Bettadapura Jayaram ◽  
René Devos ◽  
Alan Shaw ◽  
Walter Fiers
Keyword(s):  
Growth Factor ◽  
B Cell ◽  
Cell Line ◽  
Epstein Barr Virus ◽  
Autocrine Growth ◽  
Autocrine Growth Factor ◽  
Barr Virus ◽  
Interleukin 1Α ◽  
Epstein Barr ◽  
Human B Cell
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