Interleukin 1α acts as an autocrine growth factor for RPMI1788, an Epstein-Barr virus-transformed human B cell line

1988 ◽  
Vol 18 (7) ◽  
pp. 1027-1031 ◽  
Author(s):  
Peter Vandenabeele ◽  
Bettadapura Jayaram ◽  
René Devos ◽  
Alan Shaw ◽  
Walter Fiers
Keyword(s):  
Growth Factor ◽  
B Cell ◽  
Cell Line ◽  
Epstein Barr Virus ◽  
Autocrine Growth ◽  
Autocrine Growth Factor ◽  
Barr Virus ◽  
Interleukin 1Α ◽  
Epstein Barr ◽  
Human B Cell

scholarly journals Lymphotoxin is an autocrine growth factor for Epstein-Barr virus-infected B cell lines.

1993 ◽  
Vol 177 (3) ◽  
pp. 763-774 ◽  
Author(s):  
Z Estrov ◽  
R Kurzrock ◽  
E Pocsik ◽  
S Pathak ◽  
H M Kantarjian ◽  
...  
Keyword(s):  
B Cell ◽  
Cell Line ◽  
Cell Lines ◽  
Epstein Barr Virus ◽  
Lymphoblastoid Cell Line ◽  
Autocrine Growth ◽  
Autocrine Growth Factor ◽  
Barr Virus ◽  
Epstein Barr ◽  
Dose Dependent

Because human lymphotoxin (LT) was originally isolated from a lymphoblastoid cell line, we investigated the role of this molecule in three newly established Epstein-Barr virus (EBV)-infected human B cell lines. These lines were derived from acute lymphoblastic leukemia (Z-6), myelodysplastic syndrome (Z-43), and acute myelogenous leukemia (Z-55) patients who had a prior EBV infection. Each lymphoblastoid cell line had a karyotype that was different from that of the original parent leukemic cells, and all expressed B cell, but not T cell or myeloid surface markers. In all three lines, rearranged immunoglobulin heavy chain joining region (JH) bands were found, and the presence of EBV DNA was confirmed by Southern blotting. Z-6, Z-43, and Z-55 cell lines constitutively produced 192, 48, and 78 U/ml LT, respectively, as assessed by a cytotoxicity assay and antibody neutralization. Levels of tumor necrosis factor (TNF) were undetectable. Scatchard analysis revealed that all the cell lines expressed high-affinity TNF/LT receptors with receptor densities of 4197, 1258, and 1209 sites/cell on Z-6, Z-43, and Z-55, respectively. Furthermore, labeled TNF binding could be reversed by both unlabeled TNF, as well as by LT. Studies with p60 and p80 receptor-specific antibodies revealed that the three lines expressed primarily the p80 form of the TNF receptor. When studied in a clonogenic assay, exogenous LT stimulated proliferation of all three cell lines in a dose-dependent fashion at concentrations ranging from 25 to 500 U/ml. Similar results were obtained with [3H]TdR incorporation. Monoclonal anti-LT neutralizing antibodies at concentrations of 25-500 U/ml inhibited cellular multiplication in a dose-dependent manner. It is interesting that in spite of a common receptor, TNF (1,000 U/ml) had no direct effect on Z-55 cell growth, whereas it partially reversed the stimulatory effect of exogenous LT. In addition, TNF inhibited Z-6 and Z-43 cell proliferation, and its suppressive effect was reversed by exogenous LT. Both p80 and p60 forms of soluble TNF receptors suppressed the lymphoblastoid cell line proliferation and their inhibitory effect was partially reversed by LT. Our data suggest that (a) LT is an autocrine growth factor for EBV-transformed lymphoblastoid B cell lines; and (b) anti-LT antibodies, soluble TNF/LT receptors, and TNF itself can suppress the growth of lymphoblastoid cells, probably by modulating or competing with LT.(ABSTRACT TRUNCATED AT 400 WORDS)

Functional Analysis of an Epstein-Barr Virus-Nontransformed Human B Cell Line

1988 ◽  
Vol 80 (3) ◽  
pp. 139-144 ◽  
Author(s):  
Y. Yasuda ◽  
T. Iwasaki ◽  
T. Hamano ◽  
K. Nagai
Keyword(s):  
Functional Analysis ◽  
B Cell ◽  
Cell Line ◽  
Epstein Barr Virus ◽  
Barr Virus ◽  
Epstein Barr ◽  
Human B Cell

Bcl-2 and c-Myc co-operate in the Epstein – Barr virus-immortalized human B-cell line GM607 but do not confer tumorigenicity

2005 ◽  
Vol 46 (4) ◽  
pp. 581-592 ◽  
Author(s):  
Jennifer L Tomlin ◽  
Barbara-Ann Guinn ◽  
Linda Z Penn ◽  
Neil L Berinstein
Keyword(s):  
B Cell ◽  
Cell Line ◽  
Epstein Barr Virus ◽  
Barr Virus ◽  
Epstein Barr ◽  
Human B Cell
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