Effect of Amiloride on Human Bronchial Ciliary Activity in vitro

Respiration ◽  
1990 ◽  
Vol 57 (1) ◽  
pp. 37-39 ◽  
Author(s):  
G. Di Benedetto ◽  
M.T. Lopez-Vidriero ◽  
L. Carratù ◽  
S.W. Clarke
Keyword(s):  
Ciliary Activity

Physiological and hypertonic saline solutions impair ciliary activity in vitro

2000 ◽  
Vol 25 (4) ◽  
pp. 331-332
Author(s):  
W.M. Boek ◽  
N. Keles ◽  
K. Graamans ◽  
E.H. Huizing
Keyword(s):  
Hypertonic Saline ◽  
Ciliary Activity ◽  
Saline Solutions

Effects of tobacco smoke compounds on the ciliary activity of the embryo chicken trachea in vitro

Toxicology ◽  
1982 ◽  
Vol 23 (1) ◽  
pp. 41-55 ◽  
Author(s):  
Bertil Petersson ◽  
Margareta Curvall ◽  
Curt R. Enzell
Keyword(s):  
Tobacco Smoke ◽  
Ciliary Activity

Inhibition of Mucociliary Clearance of the Eustachian Tube by Leukotrienes C4 and D4

1995 ◽  
Vol 104 (3) ◽  
pp. 231-236 ◽  
Author(s):  
Tetsuya Ganbo ◽  
Tsukasa Nakajima ◽  
Ken-Ichi Hisamaisu ◽  
Hajime Inoue ◽  
Shin-Ichi Shimomura ◽  
...  
Keyword(s):  
Eustachian Tube ◽  
Mucociliary Clearance ◽  
Ciliary Activity ◽  
Leukotriene D4 ◽  
Auditory Brain Stem Response ◽  
Auditory Brain Stem ◽  
Brain Stem Response ◽  
Ear Effusion

The effect of leukotrienes C4 (LTC4) and D4 (LTD4) and prostaglandin E2 (PGE2) on mucociliary clearance of the eustachian tube was investigated in vitro and in vivo. Normal ciliated epithelium was obtained from the eustachian tube of guinea pigs and incubated separately with LTC4, LTD4, and PGE2 at concentrations of 10−8 mol/L and 10−6 mol/L. Ciliary activity was measured photoelectrically. Leukotriene D4 progressively inhibited ciliary activity, while PGE2 promoted it. Leukotriene C4 also induced ciliary inhibition. One milliliter each of 10−5 mol/L LTC4, LTD4, and PGE2 was directly injected into the tympanic bullae of chinchillas under anesthesia. The middle ears were examined by otomicroscopy, tympanometry, and auditory brain stem response over time. Clearance of middle ear effusion was delayed by LTC4 and LTD4, as compared with PGE2 and the control. These findings indicate that LTC4 and LTD4 inhibit mucociliary clearance of the eusiachian tube.

Mucociliary interaction in vitro: effects of physiological and inflammatory stimuli

1990 ◽  
Vol 68 (4) ◽  
pp. 1421-1426 ◽  
Author(s):  
Z. V. Seybold ◽  
A. T. Mariassy ◽  
D. Stroh ◽  
C. S. Kim ◽  
H. Gazeroglu ◽  
...  
Keyword(s):  
Liquid Velocity ◽  
Beat Frequency ◽  
Platelet Activating Factor ◽  
Ciliary Beat Frequency ◽  
Ciliary Activity ◽  
Base Line ◽  
Mucociliary Transport ◽  
Epithelial Surface ◽  
Surface Liquid

Mucociliary transport in the airways is governed by the interaction between ciliary activity and the depth and rheological properties of the liquids (mucus) covering the epithelial surface. A change in one of these parameters may not predict the direction and magnitude of a concomitant change in mucociliary transport. We therefore determined the effects of physiological (neurotransmitters) and pathological (inflammatory mediators) stimuli on ciliary beat frequency (CBF), surface liquid velocity (SLV), surface liquid depth (SLD), and viscoelasticity of mucus in pieces of sheep trachea (n = 5 for each treatment) mounted in a chamber such that the submucosal side was bathed with Krebs-Henseleit perfusate (KH) and the luminal side was exposed to conditioned air. SLV, SLD, and CBF were measured with a microscope provided with an electronic micrometer and strobe light. Apparent viscosity and shear elastic modulus were measured with a microcapillary method using mucus collected at the downstream end of the preparation. Control CBF, SLV, and SLD were 11.6 +/- 0.4 (SE) Hz, 91 +/- 8 micron/s, and 33 +/- 5 microns, respectively, at base line and did not change during KH perfusion for 100 min. Perfusion with both acetylcholine and epinephrine (10(-5) to 10(-3) M) produced concentration-dependent increases in mean CBF (maximum increases at 10(-3) M of 16 and 9%, P less than 0.05), whereas only acetylcholine increased mean SLV (+56% at 10(-3) M, P less than 0.05). Perfusion with platelet-activating factor (10(-7) to 10(-5) M) decreased both mean CBF and SLV in a dose-dependent fashion (-6 and -63% at 10(-5) M, P less than 0.05), whereas antigen perfusion (1:60 dilution) increased mean CBF (+10%, P less than 0.05) but decreased SLV (-47%, P less than 0.05).(ABSTRACT TRUNCATED AT 250 WORDS)

Effects of bacterial endotoxin on the ciliary activity in the in vitro eustachian tube

1987 ◽  
Vol 244 (2) ◽  
pp. 88-90 ◽  
Author(s):  
Y. Ohashi ◽  
Y. Nakai ◽  
H. Ikeoka ◽  
H. Koshimo ◽  
Y. Esaki ◽  
...  
Keyword(s):  
Eustachian Tube ◽  
Bacterial Endotoxin ◽  
Ciliary Activity

Tissue Culture of Human Adult Adenoids and of Middle Ear Mucosa

1976 ◽  
Vol 85 (3) ◽  
pp. 327-333 ◽  
Author(s):  
Ilana Drucker ◽  
Ziva Weisman ◽  
Jacob Sadé
Keyword(s):  
Middle Ear ◽  
Ciliary Activity ◽  
Organ Cultures ◽  
Middle Ear Mucosa ◽  
Monolayer Growth ◽  
Stratified Squamous Epithelium ◽  
Inducing Factors ◽  
Developmental Characteristics ◽  
Mucociliary Epithelia

The increased number of mucus producing cells as well as the presence of stratified squamous epithelium in pathological and experimental middle ear conditions, point towards the possibility of metaplastic changes of the middle ear mucosa, similar to the metaplastic capabilities of respiratory mucosae in general, as observed clinically or provoked experimentally. The purpose of this study was to develop a model of postembryonic human respiratory mucosae, in vitro, for the study of triggering or inducing factors involved in its normal and metaplastic differentiation. Explants from adenoids and middle ear mucosa were cultured, both as organ cultures and monolayers, for periods of up to two weeks, and their developmental characteristics were studied and described. Over 50% of the explants showed mitosis, epithelial and monolayer growth, ciliary activity and differentiation into ciliated and into mucus-producing cells. Adenoid explants were grown in air without and with added 5% CO2. Under the latter conditions, the proportion of explants and monolayers showing ciliary activity was 50% greater. It is concluded that this model might be suitable for further studies of the factors which control cyto-differentiation in mucociliary epithelia. Maintaining its growth for a longer period would, however, be desirable.

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