Cyclosporin-A- and Angiotensin-ll- Induced Vasoreactivity in Isolated Glomeruli and Cultured Mesangial Cells, 4 and 24 h after Renal Mass Reduction: Role of Vasodilatory Prostaglandins

Nephron ◽  
1997 ◽  
Vol 76 (3) ◽  
pp. 337-344 ◽  
Author(s):  
O. Forpomès ◽  
C. Combe ◽  
J. Cambar ◽  
M. Aparicio
Keyword(s):  
Cyclosporin A ◽  
Renal Mass ◽  
Mesangial Cells ◽  
Mass Reduction ◽  
Isolated Glomeruli

Conserved charge of glomerular and mesangial cell proteoglycans: possible role of amino acid-derived sulphate

1992 ◽  
Vol 70 (6) ◽  
pp. 843-852 ◽  
Author(s):  
Douglas M. Templeton ◽  
Aimin Wang
Keyword(s):  
Charge Density ◽  
Mesangial Cells ◽  
Heparan Sulphate ◽  
Cell Types ◽  
Sole Source ◽  
Isolated Glomeruli ◽  
Inorganic Sulphate ◽  
Multifunctional Molecules ◽  
Nontransformed Cell

Sulphation of proteoglycans was studied in isolated glomeruli and cultured rat mesangial cells. Both preparations produced heparan, dermatan, and chondroitin sulphates, recoverable both from the tissue layers and the conditioned media. The proportion of heparan sulphate made by mesangial cells was independent of the age of the culture, but declined in later passage. These preparations differed from several other nontransformed cell types studied to date in that the degree of proteoglycan sulphation was independent of the concentration of inorganic sulphate provided. Even when no exogenous sulphate was added, sulphation-dependent charge density of newly synthesized proteoglycans was conserved. Both isolated glomeruli and cultured mesangial cells produced proteoglycans with 35S-labelled sulphate esters when [35S]methionine was provided as the sole source of labelled sulphate. Conversion of methionine to cysteine and subsequent oxidation of organic sulphate via the sulphinyl pyruvate pathway is the only mechanism known in eukaryotic cells that can account for this observation. We conclude that facile oxidation of sulphur-containing amino acids can contribute to sulphation of glomerular proteoglycans and may serve to sustain the charge density of these multifunctional molecules when the supply of inorganic sulphate is otherwise compromised.Key words: renal glomerulus, proteoglycans, sulphation, heparan sulphate, basement membrane.

Role of endothelium-derived nitric oxide in hemodynamic adaptations after graded renal mass reduction

1993 ◽  
Vol 264 (6) ◽  
pp. R1254-R1259 ◽  
Author(s):  
K. A. Griffin ◽  
A. K. Bidani ◽  
J. Ouyang ◽  
V. Ellis ◽  
M. Churchill ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Renal Mass ◽  
Filtration Rate ◽  
Plasma Renin ◽  
Mass Reduction ◽  
Synthesis Inhibitor ◽  
Contralateral Kidney ◽  
Before And After ◽  
Renal Vascular

The mediator(s) of the adaptive increases in renal blood flow (RBF) and glomerular filtration rate (GFR) after renal mass reduction have not been identified. The present studies were designed to investigate the role of endothelium-derived nitric oxide (EDNO) in the hemodynamic adaptations after graded renal mass reduction. The experiments were performed in rats that had undergone a sham reduction in renal mass, uninephrectomy (UNX), or 5/6 NX (UNX plus excision of both poles of the contralateral kidney) 3-4 wk before. Measurements of RBF, GFR, renal vascular resistance (RVR), mean arterial pressure (MAP), and plasma renin concentration (PRC) were obtained before and after administration of the EDNO synthesis inhibitor NG-monomethyl-L-arginine (L-NMMA). L-NMMA (50 mg/kg bolus plus 500 micrograms.kg-1.min-1 infusion) led to significant (P < 0.01) and comparable increases in MAP (mmHg) (P < 0.01) in sham rats (117 +/- 6 to 154 +/- 6), UNX rats (112 +/- 5 to 139 +/- 7), and 5/6 NX rats (116 +/- 5 to 149 +/- 7). RVR increased significantly in all three groups (P < 0.01). The resultant decrease in RBF (ml.min-1.kg-1) was similar in sham rats (34.9 +/- 2.6 to 23.8 +/- 1.6), UNX rats (43.9 +/- 3.6 to 27.3 +/- 2.8), and 5/6 NX rats (34.6 +/- 2 to 22.3 +/- 1.6) (P < 0.01 for all groups).(ABSTRACT TRUNCATED AT 250 WORDS)

scholarly journals Potential role of circular RNA in cyclosporin A‐induced cardiotoxicity in rats

2021 ◽  
Author(s):  
Yanru Zhao ◽  
Yang Li ◽  
Dachuan Fan ◽  
Jinxiao Hou ◽  
Yunpeng Bai ◽  
...  
Keyword(s):  
Cyclosporin A ◽  
Potential Role ◽  
Circular Rna

scholarly journals Cox-2 and osteopontin in cocultured platelets and mesangial cells: Role of glucocorticoids

2000 ◽  
Vol 57 (6) ◽  
pp. 2229-2238 ◽  
Author(s):  
Margarete Goppelt-Struebe ◽  
Timo Wiedemann ◽  
Juliane Heusinger-Ribeiro ◽  
Mario Vucadinovic ◽  
Margot Rehm ◽  
...  
Keyword(s):  
Mesangial Cells ◽  
Cox 2
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