scholarly journals Plasma-Ionized Magnesium Concentration in a Child with Magnesium-Losing Tubulopathy

Nephron ◽  
1995 ◽  
Vol 71 (2) ◽  
pp. 238-239 ◽  
Author(s):  
Anita C. Truttmann ◽  
Regula Laux-End ◽  
John A.S. McGuigan ◽  
Mario G. Bianchetti
Keyword(s):  
Magnesium Concentration ◽  
Ionized Magnesium

scholarly journals Estimating serum‐ionized magnesium concentration in hemodialysis patients

2021 ◽  
Author(s):  
Christopher Holzmann‐Littig ◽  
Claudius Kuechle ◽  
Andreas Bietenbeck ◽  
Wendy McCallum ◽  
Uwe Heemann ◽  
...  
Keyword(s):  
Hemodialysis Patients ◽  
Magnesium Concentration ◽  
Ionized Magnesium

scholarly journals Determination of ionized magnesium in platelets and correlation with selected variables

1996 ◽  
Vol 42 (5) ◽  
pp. 744-748 ◽  
Author(s):  
J E Niemela ◽  
B M Snader ◽  
R J Elin
Keyword(s):  
Total Cholesterol ◽  
Inverse Correlation ◽  
Reference Interval ◽  
Cholesterol Concentration ◽  
Magnesium Concentration ◽  
Serum Electrolytes ◽  
Ionized Magnesium ◽  
And Gender ◽  
Dissociation Constant Kd

Abstract We describe a method for determining the intracellular ionized magnesium concentration ([Mg2+]i) in platelets by using the fluorescent probe FURAPTRA. We determined the dissociation constant (KD) of FURAPTRA for Mg2+ (2.26 +/- 0.29 mmol/L), within-day assay variability (CV = 6.8%), among-day intraindividual variability (CV = 11.0%), variability after a 4-h delay in processing the blood specimen (t = 1.2, P >0.2; F = 6.2, P <0.02), and the reference interval (0.23-0.59 mmol/L) for this assay. We also evaluated the correlation between platelet [Mg2+]i and concentrations of selected serum electrolytes, proteins, and total cholesterol; age; body mass index; and gender. Only the inverse correlation between platelet [Mg2+]i and serum total cholesterol concentration in men was significant (r=-0.66, P <0.005).

scholarly journals Circulating Ionized Magnesium as a Measure of Supplement Bioavailability: Results from a Pilot Study for Randomized Clinical Trial

Nutrients ◽  
2020 ◽  
Vol 12 (5) ◽  
pp. 1245 ◽  
Author(s):  
Jiada Zhan ◽  
Taylor C. Wallace ◽  
Sarah J. Butts ◽  
Sisi Cao ◽  
Velarie Ansu ◽  
...  
Keyword(s):  
Pilot Study ◽  
Whole Blood ◽  
Area Under The Curve ◽  
Oral Intake ◽  
Magnesium Content ◽  
Magnesium Concentration ◽  
Rank Test ◽  
Ionized Magnesium ◽  
Signed Rank Test ◽  
Total Magnesium

Oral supplementation may improve the dietary intake of magnesium, which has been identified as a shortfall nutrient. We conducted a pilot study to evaluate appropriate methods for assessing responses to the ingestion of oral magnesium supplements, including ionized magnesium in whole blood (iMg2+) concentration, serum total magnesium concentration, and total urinary magnesium content. In a single-blinded crossover study, 17 healthy adults were randomly assigned to consume 300 mg of magnesium from MgCl2 (ReMag®, a picosized magnesium formulation) or placebo, while having a low-magnesium breakfast. Blood and urine samples were obtained for the measurement of iMg2+, serum total magnesium, and total urine magnesium, during 24 h following the magnesium supplement or placebo dosing. Bioavailability was assessed using area-under-the-curve (AUC) as well as maximum (Cmax) and time-to-maximum (Tmax) concentration. Depending on normality, data were expressed as the mean ± standard deviation or median (range), and differences between responses to MgCl2 or placebo were measured using the paired t-test or Wilcoxon signed-rank test. Following MgCl2 administration versus placebo administration, we observed significantly greater increases in iMg2+ concentrations (AUC = 1.51 ± 0.96 vs. 0.84 ± 0.82 mg/dL•24h; Cmax = 1.38 ± 0.13 vs. 1.32 ± 0.07 mg/dL, respectively; both p < 0.05) but not in serum total magnesium (AUC = 27.00 [0, 172.93] vs. 14.55 [0, 91.18] mg/dL•24h; Cmax = 2.38 [1.97, 4.01] vs. 2.24 [1.98, 4.31] mg/dL) or in urinary magnesium (AUC = 201.74 ± 161.63 vs. 139.30 ± 92.84 mg•24h; Cmax = 26.12 [12.91, 88.63] vs. 24.38 [13.51, 81.51] mg/dL; p > 0.05). Whole blood iMg2+ may be a more sensitive measure of acute oral intake of magnesium compared to serum and urinary magnesium and may be preferred for assessing supplement bioavailability.

Cytosolic Ionized Magnesium Concentration in End-Stage Renal Disease

ASAIO Journal ◽  
1993 ◽  
Vol 39 (3) ◽  
pp. M614-M617 ◽  
Author(s):  
Charles J. Kaupke ◽  
Xin J. Zhou ◽  
N. D. Vaziri
Keyword(s):  
Renal Disease ◽  
End Stage Renal Disease ◽  
Magnesium Concentration ◽  
Ionized Magnesium ◽  
Stage Renal Disease ◽  
End Stage

scholarly journals Evaluation of Plasma-Ionized Magnesium Concentration in 122 Dogs with Diabetes Mellitus: A Retrospective Study

2004 ◽  
Vol 18 (5) ◽  
pp. 612-617 ◽  
Author(s):  
Suzanne C. Fincham ◽  
Kenneth J. Drobatz ◽  
Tracey N. Gillespie ◽  
Rebecka S. Hess
Keyword(s):  
Diabetes Mellitus ◽  
Retrospective Study ◽  
Magnesium Concentration ◽  
Ionized Magnesium

31P nuclear magnetic resonance and zero-point titration compared for measuring free magnesium concentration in erythrocytes

1991 ◽  
Vol 37 (12) ◽  
pp. 2076-2080 ◽  
Author(s):  
W B Geven ◽  
G M Vogels-Mentink ◽  
J L Willems ◽  
C H v Os ◽  
C W Hilbers ◽  
...  
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
31P Nmr ◽  
Zero Point ◽  
Magnesium Concentration ◽  
Cell Water ◽  
Ionized Magnesium ◽  
31P Nuclear Magnetic Resonance ◽  
L Cell ◽  
Magnesium Loss

Abstract Intracellular ionized magnesium concentrations ([Mg2+]i) were measured in erythrocytes by 31P nuclear magnetic resonance (NMR) and zero-point titration in 14 controls and seven patients with renal magnesium loss. The mean intracellular ionized magnesium concentration in controls measured by 31P NMR was 0.20 (SD 0.03) mmol/L cell water, compared with 0.55 (SD 0.12) mmol/L cell water by zero-point titration. Total erythrocyte magnesium content measured with the lysate method was 0.63 mmol/L cell water higher than estimated by 31P NMR, probably because not all magnesium complexes are fully visible to the NMR technique. We found a positive correlation between plasma ultrafiltrable magnesium and [Mg2+]i irrespective of the [Mg2+]i assay used. [Mg2+]i measured with 31P NMR correlated modestly but significantly with [Mg2+]i determined by zero-point titration (r = 0.58, P less than 0.02). Washing erythrocytes before the zero-point titration decreased the ATP content and the cell water fraction, which led to overestimation of [Mg2+]i by zero-point titration. Although absolute values for [Mg2+]i differ with the assay used, both methods determined significantly lower values for [Mg2+]i in patients with isolated renal magnesium loss.

pH Effects on Measurements of Ionized Calcium and Ionized Magnesium in Blood

2002 ◽  
Vol 126 (8) ◽  
pp. 947-950 ◽  
Author(s):  
Sihe Wang ◽  
Elizabeth H. McDonnell ◽  
Frank A. Sedor ◽  
John G. Toffaletti
Keyword(s):  
Whole Blood ◽  
Rate Of Change ◽  
Ionized Calcium ◽  
Magnesium Concentration ◽  
Blood Gas ◽  
Ph Change ◽  
Ionized Magnesium ◽  
Ph Values ◽  
Wide Range ◽  
Blood Specimens

Abstract Context.—It is well known that the concentration of ionized calcium in blood is affected by the pH of the specimen, since hydrogen ions compete with calcium for binding sites on albumin and other proteins. However, the relationship between pH and ionized magnesium concentration is not as well characterized. Objective.—To determine the effects of pH on ionized magnesium concentration over a wide range of pH values in serum or plasma. Design.—Both ionized calcium and ionized magnesium concentrations were measured in 3 sets of samples. (1) Pools of serum or whole blood at different pH values (7.20–7.60) achieved by adding a constant volume of acid or base (diluted solutions of either hydrochloric acid or sodium hydroxide) plus saline. These pools consisted of 2 serum and 3 heparinized whole blood pools collected from leftover blood remaining in clinical specimens in the Clinical Chemistry and Blood Gas Laboratories, respectively, at Duke University Medical Center. (2) Five whole blood specimens obtained from apparently healthy individual donors. (3) Twenty-six whole blood specimens obtained from individual patients (leftover blood from the Blood Gas Laboratory) in which pH was varied by in vitro loss or gain of carbon dioxide. Results.—Both ionized calcium and ionized magnesium concentrations decreased as the pH in the specimen increased, indicating the stronger binding of these ions with proteins in the more alkaline environment. Conclusion.—We conclude that the rate of change of ionized magnesium concentration with pH change (0.12 mmol/L per pH unit) is significantly less than that of ionized calcium (0.36 mmol/L per pH unit). Furthermore, our findings indicate that if adjustment to pH 7.40 is necessary, the ionized magnesium test results need to be adjusted when pH is markedly abnormal, as is sometimes done for ionized calcium.

Blood Ionized Magnesium Concentration in Preterm Fetal Sheep Increases during Umbilical Cord Occlusion 

2000 ◽  
Vol 2000 (4) ◽  
pp. NA-NA
Author(s):  
J D Reynolds ◽  
S. Punnahitananda ◽  
Y. Wang ◽  
M. Hopkins ◽  
F. Dexter ◽  
...  
Keyword(s):  
Umbilical Cord ◽  
Magnesium Concentration ◽  
Fetal Sheep ◽  
Ionized Magnesium
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