scholarly journals Effect of Recombinant Human Erythropoietin on Nutritional Status and Plasma Lipids in Uremic Patients

Nephron ◽  
1992 ◽  
Vol 60 (2) ◽  
pp. 249-249 ◽  
Author(s):  
B. Viron ◽  
R. Donsimoni ◽  
C. Michel ◽  
R. Al Khayat ◽  
F. Mignon
Keyword(s):  
Nutritional Status ◽  
Recombinant Human Erythropoietin ◽  
Plasma Lipids ◽  
Human Erythropoietin ◽  
Uremic Patients

Erythropoietin Improves Signaling through Tyrosine Phosphorylation in Platelets from Uremic Patients

1999 ◽  
Vol 82 (10) ◽  
pp. 1312-1317 ◽  
Author(s):  
Eva Estebanell ◽  
Aleix Cases ◽  
José López-Pedret ◽  
Ricardo Castillo ◽  
Antonio Ordinas ◽  
...  
Keyword(s):  
Gel Electrophoresis ◽  
Recombinant Human Erythropoietin ◽  
Ethylenediaminetetraacetic Acid ◽  
Platelet Rich Plasma ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Polyacrylamide Gel ◽  
Platelet Response ◽  
Human Erythropoietin ◽  
Uremic Patients

SummaryErythropoietin has shown to be effective in the correction of the hemostatic defect present in uremic patients. We have investigated the possible effect of recombinant human erythropoietin (rHuEPO) on the signaling processes occurring in platelets. Platelet suspensions were obtained from hemodialyzed patients before and after at least one month of initiating treatment with rHuEPO. Aliquots of non-activated or thrombin-activated platelets were treated to obtain platelet lysates or processed to extract platelet cytoskeleton. Samples were resolved by 8% SDS-polyacrylamide gel electrophoresis followed by Western blotting. After thrombin activation, proteins p120, p85, p78, p75, pp62, pp60, p59, p58, p56, p54 and p52 associated with the Triton-insoluble cytoskeletal fraction appeared phosphorylated in control profiles. In profiles from platelets obtained from uremic patients before treatment with rHuEPO, only proteins p58 and p56 appeared clearly and p54 was slightly phosphorylated. However, in platelets from the same patients under rHuEPO treatment, thrombin-induced phosphorylation improved to levels even above those observed in control profiles. Specially, the band at 54KDa appeared consistently more phosphorylated in all the patients under rHuEPO treatment. Although it is accepted that part of the hemostatic effect of erythropoietin is mediated by an increase in hematocrit, our study suggests that it enhances platelet signaling in uremic platelets which may explain the improvement of platelet response to activating stimulus before clinically noticeable elevation of hematocrit. Abbrevations: rHuEPO = recombinant human erythropoietin; SDS-PAGE = sodium dodecyl sulphate-polyacrylamide gel electrophoresis; CPD = citrate/phosphate/dextrose; PRP = platelet-rich plasma; HBSS = Hanks’ balanced salt solution; EGTA = ethylene glycol bis (β-aminoethylether)-N,N,N’,N’-tetraacetic acid; EDTA = ethylenediaminetetraacetic acid, PMSF = phenylmethylsulphonyl fluoride, ECL = enhanced chemiluminiscence

scholarly journals Antiplatelet therapy and development of hypertension induced by recombinant human erythropoietin in uremic patients

1994 ◽  
Vol 45 (3) ◽  
pp. 845-851 ◽  
Author(s):  
Francisco Caravaca ◽  
José L. Pizarro ◽  
Manuel Arrobas ◽  
Juan J. Cubero ◽  
María C. García ◽  
...  
Keyword(s):  
Antiplatelet Therapy ◽  
Recombinant Human Erythropoietin ◽  
Human Erythropoietin ◽  
Uremic Patients

scholarly journals Recombinant human erythropoietin treatment improves platelet function in uremic patients

1992 ◽  
Vol 42 (3) ◽  
pp. 668-672 ◽  
Author(s):  
Aleix Cases ◽  
Gines Escolar ◽  
Juan Carlos Reverter ◽  
Antonio Ordinas ◽  
Jose Lopez-Pedret ◽  
...  
Keyword(s):  
Platelet Function ◽  
Recombinant Human Erythropoietin ◽  
Human Erythropoietin ◽  
Erythropoietin Treatment ◽  
Uremic Patients

Effect of Androgens on Anemia and Malnutrition in Renal Failure: Implications for Patients on Peritoneal Dialysis

2001 ◽  
Vol 21 (1) ◽  
pp. 1-15 ◽  
Author(s):  
Juan F. Navarro ◽  
Carmen Mora
Keyword(s):  
Peritoneal Dialysis ◽  
Nutritional Status ◽  
Data Extraction ◽  
Recombinant Human Growth Hormone ◽  
Human Growth ◽  
Dialysis Patients ◽  
Nutritional Parameters ◽  
Beneficial Effects ◽  
Human Erythropoietin ◽  
Uremic Patients

Objective To analyze the implications of the potential use of androgens in peritoneal dialysis patients, focusing on their effects on hematologic and nutritional parameters. This manuscript reviews the different compounds for clinical use, dosage schedules, adverse effects, and how therapy with androgens might be used to treat anemia and malnutrition in these dialysis patients. Data Sources Studies in the literature dealing with the effects of androgens on hematologic and nutritional parameters, and their role in uremic anemia and malnutrition. Study Selection Studies in which uremic patients received androgens as therapy for anemia or malnutrition. Data Extraction Data were abstracted from all of these studies. Results This review shows that androgens are anabolic substances that also have significant actions on erythropoiesis. A number of clinical studies in uremic patients have found that these compounds have beneficial effects on hematologic parameters and nutritional status, similarly to other therapies, such as recombinant human erythropoietin and recombinant human growth hormone, respectively. Conclusions Androgens have been shown to have a beneficial effect on anemia due to renal disease and on nutritional status in uremic patients. Further studies need to be done with larger groups of patients. Objectives for additional research are suggested.

Effect of Recombinant Human Erythropoietin Treatment in Uremic Patients on Oxygen Affinity of Hemoglobin

Nephron ◽  
1994 ◽  
Vol 66 (2) ◽  
pp. 147-152 ◽  
Author(s):  
P. Brunet ◽  
Y. Berland ◽  
T. Merzouk ◽  
D. Vanuxem ◽  
M. Badier ◽  
...  
Keyword(s):  
Recombinant Human Erythropoietin ◽  
Oxygen Affinity ◽  
Human Erythropoietin ◽  
Erythropoietin Treatment ◽  
Oxygen Affinity Of Hemoglobin ◽  
Uremic Patients
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