scholarly journals Antiplatelet therapy and development of hypertension induced by recombinant human erythropoietin in uremic patients

1994 ◽  
Vol 45 (3) ◽  
pp. 845-851 ◽  
Author(s):  
Francisco Caravaca ◽  
José L. Pizarro ◽  
Manuel Arrobas ◽  
Juan J. Cubero ◽  
María C. García ◽  
...  
Keyword(s):  
Antiplatelet Therapy ◽  
Recombinant Human Erythropoietin ◽  
Human Erythropoietin ◽  
Uremic Patients

scholarly journals Effect of Recombinant Human Erythropoietin on Nutritional Status and Plasma Lipids in Uremic Patients

Nephron ◽  
1992 ◽  
Vol 60 (2) ◽  
pp. 249-249 ◽  
Author(s):  
B. Viron ◽  
R. Donsimoni ◽  
C. Michel ◽  
R. Al Khayat ◽  
F. Mignon
Keyword(s):  
Nutritional Status ◽  
Recombinant Human Erythropoietin ◽  
Plasma Lipids ◽  
Human Erythropoietin ◽  
Uremic Patients

Erythropoietin Improves Signaling through Tyrosine Phosphorylation in Platelets from Uremic Patients

1999 ◽  
Vol 82 (10) ◽  
pp. 1312-1317 ◽  
Author(s):  
Eva Estebanell ◽  
Aleix Cases ◽  
José López-Pedret ◽  
Ricardo Castillo ◽  
Antonio Ordinas ◽  
...  
Keyword(s):  
Gel Electrophoresis ◽  
Recombinant Human Erythropoietin ◽  
Ethylenediaminetetraacetic Acid ◽  
Platelet Rich Plasma ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Polyacrylamide Gel ◽  
Platelet Response ◽  
Human Erythropoietin ◽  
Uremic Patients

SummaryErythropoietin has shown to be effective in the correction of the hemostatic defect present in uremic patients. We have investigated the possible effect of recombinant human erythropoietin (rHuEPO) on the signaling processes occurring in platelets. Platelet suspensions were obtained from hemodialyzed patients before and after at least one month of initiating treatment with rHuEPO. Aliquots of non-activated or thrombin-activated platelets were treated to obtain platelet lysates or processed to extract platelet cytoskeleton. Samples were resolved by 8% SDS-polyacrylamide gel electrophoresis followed by Western blotting. After thrombin activation, proteins p120, p85, p78, p75, pp62, pp60, p59, p58, p56, p54 and p52 associated with the Triton-insoluble cytoskeletal fraction appeared phosphorylated in control profiles. In profiles from platelets obtained from uremic patients before treatment with rHuEPO, only proteins p58 and p56 appeared clearly and p54 was slightly phosphorylated. However, in platelets from the same patients under rHuEPO treatment, thrombin-induced phosphorylation improved to levels even above those observed in control profiles. Specially, the band at 54KDa appeared consistently more phosphorylated in all the patients under rHuEPO treatment. Although it is accepted that part of the hemostatic effect of erythropoietin is mediated by an increase in hematocrit, our study suggests that it enhances platelet signaling in uremic platelets which may explain the improvement of platelet response to activating stimulus before clinically noticeable elevation of hematocrit. Abbrevations: rHuEPO = recombinant human erythropoietin; SDS-PAGE = sodium dodecyl sulphate-polyacrylamide gel electrophoresis; CPD = citrate/phosphate/dextrose; PRP = platelet-rich plasma; HBSS = Hanks’ balanced salt solution; EGTA = ethylene glycol bis (β-aminoethylether)-N,N,N’,N’-tetraacetic acid; EDTA = ethylenediaminetetraacetic acid, PMSF = phenylmethylsulphonyl fluoride, ECL = enhanced chemiluminiscence

scholarly journals Recombinant human erythropoietin treatment improves platelet function in uremic patients

1992 ◽  
Vol 42 (3) ◽  
pp. 668-672 ◽  
Author(s):  
Aleix Cases ◽  
Gines Escolar ◽  
Juan Carlos Reverter ◽  
Antonio Ordinas ◽  
Jose Lopez-Pedret ◽  
...  
Keyword(s):  
Platelet Function ◽  
Recombinant Human Erythropoietin ◽  
Human Erythropoietin ◽  
Erythropoietin Treatment ◽  
Uremic Patients

Effect of Recombinant Human Erythropoietin Treatment in Uremic Patients on Oxygen Affinity of Hemoglobin

Nephron ◽  
1994 ◽  
Vol 66 (2) ◽  
pp. 147-152 ◽  
Author(s):  
P. Brunet ◽  
Y. Berland ◽  
T. Merzouk ◽  
D. Vanuxem ◽  
M. Badier ◽  
...  
Keyword(s):  
Recombinant Human Erythropoietin ◽  
Oxygen Affinity ◽  
Human Erythropoietin ◽  
Erythropoietin Treatment ◽  
Oxygen Affinity Of Hemoglobin ◽  
Uremic Patients

Assessment of Iron Status by Erythrocyte Ferritin in Uremic Patients With or Without Recombinant Human Erythropoietin Therapy

1992 ◽  
Vol 20 (3) ◽  
pp. 249-254 ◽  
Author(s):  
Francisco Caravaca ◽  
Jose M. Vagace ◽  
Angel Aparicio ◽  
Jorge Groiss ◽  
Jose L. Pizarro ◽  
...  
Keyword(s):  
Recombinant Human Erythropoietin ◽  
Iron Status ◽  
Human Erythropoietin ◽  
Uremic Patients
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