Immunoelectron Microscopic Localization of Fibronectin and Complement to ‘Dense Bodies’ in Bowman’s Capsule and the Glomerular Basement Membrane in Membranous Nephropathy

Nephron ◽  
1991 ◽  
Vol 58 (4) ◽  
pp. 483-484 ◽  
Author(s):  
Michael Slater
Keyword(s):  
Basement Membrane ◽  
Glomerular Basement Membrane ◽  
Membranous Nephropathy ◽  
Microscopic Localization ◽  
Dense Bodies ◽  
Bowman's Capsule

Membranous nephropathy

2010 ◽  
pp. 3985-3988
Author(s):  
Dwomoa Adu
Keyword(s):  
Prostate Cancer ◽  
Systemic Lupus Erythematosus ◽  
Nephrotic Syndrome ◽  
Basement Membrane ◽  
Autoimmune Diseases ◽  
Outer Surface ◽  
Glomerular Basement Membrane ◽  
Lupus Erythematosus ◽  
Membranous Nephropathy ◽  
Systemic Lupus

Membranous nephropathy, which accounts for 20 to 30% of cases of the nephrotic syndrome in adults, is defined histologically by the presence of subepithelial immune deposits on the outer surface of the glomerular basement membrane. The immune mechanisms that lead to this are uncertain, and most cases are of unknown cause (idiopathic), but the condition can be associated with autoimmune diseases (systemic lupus erythematosus), malignancy (in 10% of cases, most commonly lung and prostate cancer), drugs, and infections....

Ovine Mesangiocapillary Glomerulonephritis Type I and Crescent Formation

1990 ◽  
Vol 27 (1) ◽  
pp. 26-34 ◽  
Author(s):  
P. F. Frelier ◽  
D. L. Armstrong ◽  
J. Pritchard
Keyword(s):  
Basement Membrane ◽  
Glomerular Basement Membrane ◽  
Inflammatory Cells ◽  
Suitable Model ◽  
Type I ◽  
Human Beings ◽  
Crescent Formation ◽  
Mesangiocapillary Glomerulonephritis ◽  
Bowman's Capsule

Morphologic examination of four Finnish Landrace mixed-breed lambs, 27 to 35 days of age, affected with mesangiocapillary glomerulonephritis type 1, demonstrated a progressive glomerulonephritis. By 27 days of age, three lambs had crescents in 58 to 93% of glomeruli. These three lambs were also uremic. The accelerated rate of crescent formation was attributed to infiltrating polymorphonuclear leukocytes and monocytes, the result of discontinuities (gaps) in the glomerular basement membrane, and to the loss of the integrity of Bowman's capsule. In the three lambs, platelets were identified adjacent to the endothelium or denuded glomerular basement membrane. Two distinctly different types of crescents were noted, apparently dependent on the integrity of Bowman's capsule. One type resulted from the influx of inflammatory cells and dissociation of parietal epithelial cells from Bowman's capsule. The other type was more extensive and contained collagen and was associated with damage to Bowman's capsule resulting in cellular infiltration from the interstitium and sclerosis. Based on morphologic similarities, ovine mesangiocapillary glomerulonephritis is a suitable model for studying the pathogenesis and treatment of mesangiocapillary glomerulonephritis type 1 in human beings.

Evolution of Membranous Nephropathy into Anti-Glomerular-Basement-Membrane Glomerulonephritis

1974 ◽  
Vol 290 (24) ◽  
pp. 1340-1344 ◽  
Author(s):  
John Klassen ◽  
Charles Elwood ◽  
Allan L. Grossberg ◽  
Felix Milgrom ◽  
Mario Montes ◽  
...  
Keyword(s):  
Basement Membrane ◽  
Glomerular Basement Membrane ◽  
Membranous Nephropathy

scholarly journals Membranous nephropathy followed by anti-glomerular basement disease: A case report and review of clinical presentation and treatment

2018 ◽  
Vol 6 ◽  
pp. 2050313X1880762
Author(s):  
Claudius Speer ◽  
Matthias Martin Gaida ◽  
Rüdiger Waldherr ◽  
Christian Nusshag ◽  
Florian Kälble ◽  
...  
Keyword(s):  
Basement Membrane ◽  
Glomerular Basement Membrane ◽  
Membranous Nephropathy ◽  
Clinical Presentation ◽  
Rapidly Progressive Glomerulonephritis ◽  
Renal Vein Thrombosis ◽  
Simultaneous Occurrence ◽  
Rapid Decline ◽  
Crescent Formation ◽  
Vein Thrombosis

Membranous nephropathy is a common cause of nephrotic syndrome in adults and can be primary or secondary through autoimmune disease, medication, infection, or malignancy. Rapidly progressive glomerulonephritis with crescent formation is rare in patients with membranous nephropathy. Thus, in cases with rapid decline in renal function, after excluding complications such as malignant hypertension, acute hypersensitivity interstitial nephritis, and bilateral renal vein thrombosis, the simultaneous occurrence of a superimposed glomerulonephritis should be considered. We report a 55-year-old man suffering from a biopsy-confirmed primary membranous nephropathy, who developed rapidly progressive glomerulonephritis with anti-glomerular basement membrane antibodies after being affected with membranous nephropathy for 8 years. The kidney biopsy revealed a concurrence of membranous nephropathy and anti-glomerular basement membrane disease. Clinical presentation and treatment of membranous nephropathy followed by anti-glomerular basement membrane disease are discussed based on our observation with promising follow-up.

scholarly journals Immunoelectron microscopic (IEM) studies on glutaraldehyde-fixed renal specimen.

1984 ◽  
Vol 32 (5) ◽  
pp. 501-509 ◽  
Author(s):  
N Shindo ◽  
E Kobayashi ◽  
M Okada
Keyword(s):  
Basement Membrane ◽  
Light Microscopy ◽  
Glomerular Basement Membrane ◽  
Membranous Nephropathy ◽  
Renal Pathology ◽  
Precise Location ◽  
Routine Diagnosis ◽  
Dense Deposits ◽  
Renal Biopsies ◽  
Glomerular Lesions

Immunofluorescent microscopy (IF) has become an essential tool in the routine diagnosis of renal pathology. It is thought that glomerular IF patterns represent different forms of immunologically mediated glomerular lesions. However, in order to preserve antigenicity IF is usually done on frozen, unfixed specimen by light microscopy and it is difficult to locate target macromolecules in tissue with precision. In order to establish the precise location of such macromolecules in glomeruli in relation to the ultrastructure, we undertook an immunoelectron microscopic (IEM) study on renal biopsies. Percutaneously biopsied tiny specimen was fixed in glutaraldehyde and processed with protease. Using peroxidase-labeled antisera, a direct IEM was done. With this technique, target macromolecules corresponded well not only to electron-dense deposits but also to IF patterns. In one case of membranous nephropathy, immunoglobulin (Ig) was found to be outside of the glomerular basement membrane (GBM), while IgA was seen to be inside the GBM. For this same case, a similar localization of immunoglobulins was not revealed by IF. In conclusion, IEM is a useful technique that can broaden our knowledge of pathogenic mechanisms in glomerular disease.

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