Effect of 1α-Hydroxyvitamin D3 and Dietary Calcium and Phosphate on the Aortic Mineral Content in Rabbits with Mild Azotemia

Nephron ◽  
1983 ◽  
Vol 34 (3) ◽  
pp. 185-191 ◽  
Author(s):  
Erling Tvedegaard ◽  
Ole Ladefoged ◽  
Maja Nielsen ◽  
Ole Kamstrup
Keyword(s):  
Mineral Content ◽  
Dietary Calcium ◽  
Hydroxyvitamin D

Associations of Dietary Calcium, Vitamin D, Milk Intakes, and 25-Hydroxyvitamin D With Bone Mass in Spanish Adolescents: The HELENA Study

2013 ◽  
Vol 16 (1) ◽  
pp. 110-117 ◽  
Author(s):  
Theodora Mouratidou ◽  
German Vicente-Rodriguez ◽  
Luis Gracia-Marco ◽  
Inge Huybrechts ◽  
Isabelle Sioen ◽  
...  
Keyword(s):  
Vitamin D ◽  
Bone Mass ◽  
Dietary Calcium ◽  
Hydroxyvitamin D ◽  
25 Hydroxyvitamin D ◽  
Helena Study

scholarly journals Response of the 5′-flanking region of the human 25-hydroxyvitamin D 1α-hydroxylase gene to physiological stimuli using a transgenic mouse model

2005 ◽  
Vol 34 (1) ◽  
pp. 237-245 ◽  
Author(s):  
I Hendrix ◽  
P H Anderson ◽  
J L Omdahl ◽  
B K May ◽  
H A Morris
Keyword(s):  
Gene Expression ◽  
Vitamin D ◽  
Vitamin D Deficiency ◽  
Transgenic Mouse ◽  
Reporter Gene ◽  
Dietary Calcium ◽  
Mrna Levels ◽  
Hydroxyvitamin D ◽  
25 Hydroxyvitamin D ◽  
Flanking Region

The enzyme 25-hydroxyvitamin D 1α-hydroxylase, or CYP27B1, is the key enzyme in the two-step activation process of vitamin D to 1,25-dihydroxyvitamin D (1,25D). While a number of regulators of the renal CYP27B1 enzyme activity have been recognized for some years, their underlying molecular mechanisms remain largely unknown, and the DNA regions involved in the in vivo regulation of gene expression by these factors have not been delineated. We have generated a transgenic mouse line that expresses 1501 bp of 5′ flanking region together with 44 bp of 5′ untranslated region of the human CYP27B1 gene fused to the firefly luciferase reporter gene. Animals expressing the luciferase gene demonstrated that both luciferase protein and mRNA for CYP27B1 were localized to proximal convoluted tubule cells of the kidney. In 2-week-old animals, the expression of the transgene and the endogenous CYP27B1 mRNA levels in the kidney were highest and fell with increasing age. Both reporter gene expression and CYP27B1 mRNA levels were downregulated in response to increasing amounts of dietary calcium in a dose-dependent manner. Vitamin D deficiency resulted in an increase in both the reporter gene and CYP27B1 expression. Interestingly, the increase in CYP27B1 mRNA levels was substantially higher than the increase in reporter gene expression, suggesting either that there is a post-transcriptional mechanism that increases the amount of CYP27B1 mRNA or that other regulatory elements are required to maximize the effect of vitamin D deficiency. These findings demonstrate that the 1501 bp 5′ flanking region of the CYP27B1 gene directs expression to the proximal convoluted tubules of the kidney and is responsible for increasing transcriptional activity when dietary calcium and vitamin D levels are depleted. It also responds in the kidney to the physiological regulators of development and ageing.

scholarly journals Dietary Calcium to Digestible Phosphorus Ratio for Optimal Growth Performance and Bone Mineralization in Growing and Finishing Pigs

Animals ◽  
2020 ◽  
Vol 10 (2) ◽  
pp. 178 ◽  
Author(s):  
Patrick Schlegel ◽  
Andreas Gutzwiller
Keyword(s):  
Growth Performance ◽  
Mineral Content ◽  
Bone Mineralization ◽  
Dietary Calcium ◽  
Phosphate Removal ◽  
Volumetric Density ◽  
Dietary Phosphorus ◽  
Maximal Load ◽  
Bone Properties ◽  
Bone Ash

Within the context of maximizing the use of dietary phosphorus, a growing-finishing pig study was conducted to determine the optimal total dietary calcium (Ca) to digestible phosphorus (dP) ratio and to verify the possibility of mineral phosphate removal during the finishing period on growth performance and mineral status. The potential for replacing chemical and mechanical bone properties by dual energy X-ray absorptiometry (DXA) measures on non-dissected feet was also verified. Three Ca to dP ratios (2.2:1, 2.5:1 and 2.8:1) within two dP levels (P+, P–) were fed during 91 days to 84 pigs. The grower and finisher P+ diets contained 3.0 and 2.4 and P– diets contained 2.5 and 1.7 g dP/kg, respectively. Growth performance and blood serum mineral content were independent of treatments, except that 2.2:1 impaired finisher feed conversion ratio compared to 2.5:1 and 2.8:1. Urinary P concentration increased by 37% in 2.2:1 compared to 2.5:1 and 2.8:1. Maximal load on bone and DXA mineral density were reduced in 2.2:1 compared to 2.8:1. Bone ash and volumetric density were reduced in 2.2:1 and 2.5:1 compared to 2.8:1. Diet P– reduced bone ash, maximal load, volumetric density and DXA bone mineral content and density. No interaction was observed between Ca and dP level. Therefore, 2.2:1 was insufficient for an efficient metabolic use of P, 2.5:1 was sufficient to maximize growth performance and 2.8:1 further improved bone mineralization. Increasing dietary Ca did not impair bone zinc content. Diets P– without supplemented mineral phosphates during the finisher period resulted, per pig, in a decrease of its use by 65% and of the calculated P excretion by 41%, without impaired growth performance. Finally, DXA data responded to dietary treatments as did labor intensive chemical and mechanical bone properties.

scholarly journals Dietary Calcium and Serum 25-Hydroxyvitamin D Status in Relation to BMD Among U.S. Adults

2009 ◽  
Vol 24 (5) ◽  
pp. 935-942 ◽  
Author(s):  
Heike A Bischoff-Ferrari ◽  
Douglas P Kiel ◽  
Bess Dawson-Hughes ◽  
John E Orav ◽  
Ruifeng Li ◽  
...  
Keyword(s):  
Dietary Calcium ◽  
Hydroxyvitamin D ◽  
25 Hydroxyvitamin D

Bone mineral content and serum 25-hydroxyvitamin D concentration in breast-fed infants with and without supplemental vitamin D

1981 ◽  
Vol 98 (5) ◽  
pp. 696-701 ◽  
Author(s):  
Frank R. Greer ◽  
John E. Searcy ◽  
Ronald S. Levin ◽  
Jean J. Steichen ◽  
Paule Steichen Asch ◽  
...  
Keyword(s):  
Vitamin D ◽  
Bone Mineral Content ◽  
Bone Mineral ◽  
Mineral Content ◽  
Hydroxyvitamin D ◽  
Supplemental Vitamin ◽  
25 Hydroxyvitamin D ◽  
Breast Fed

A STUDY OF DIETARY CALCIUM AND OTHER NUTRIENTS IN IDIOPATHIC RENAL CALCIUM STONE FORMERS WITH LOW BONE MINERAL CONTENT

1998 ◽  
pp. 654-657 ◽  
Author(s):  
ALBERTO TRINCHIERI ◽  
ROBERTA NESPOLI ◽  
FABIO OSTINI ◽  
FABRIZIO ROVERA ◽  
GIANPAOLO ZANETTI ◽  
...  
Keyword(s):  
Bone Mineral Content ◽  
Bone Mineral ◽  
Mineral Content ◽  
Dietary Calcium ◽  
Calcium Stone ◽  
Stone Formers

scholarly journals Effects of supplemental phytase on the mineral content in tibia of broilers fed different cereal based diets

2011 ◽  
Vol 50 (No. 2) ◽  
pp. 68-73 ◽  
Author(s):  
J. Pintar ◽  
M. Bujan ◽  
B. Homen ◽  
K. Gazić ◽  
M. Sikirić ◽  
...  
Keyword(s):  
Soybean Meal ◽  
Mineral Content ◽  
Calcium Content ◽  
Dietary Calcium ◽  
Mineral Levels

A 21-day experiment with day-old broilers was conducted in order to assess the effect of phytase supplementation to cereal-soybean meal based diets on the mineral content in tibia (Ca, P, Fe, Mg, Cd, Zn). Diets were formulated to contain 4 different cereals (maize, wheat, triticale and barley), 2 levels of dietary calcium (0.6 and 1.0%) and 3 levels of supplemental phytase (0, 500 and 1 000 PU/kg). Supplemental phytase increased the mineral levels in tibia. A statistically significant increase was observed for Fe (P < 0.024) and Mg (P < 0.024), but not for Ca, P, Cd and Zn contents in tibia. In the case of Fe and Mg contents significant cereal × phytase interactions were also observed (P < 0.036 and 0.002, respectively). Cereals influenced the contents of Fe (P < 0.0001), Mg (P < 0.0001), Cd (P < 0.0001) and Zn (P < 0.003) while dietary calcium levels affected only calcium content (P < 0.008).  

scholarly journals Dietary calcium does not interact with vitamin D3 in terms of determining the response and catabolism of serum 25-hydroxyvitamin D during winter in older adults

2014 ◽  
Vol 99 (6) ◽  
pp. 1414-1423 ◽  
Author(s):  
Kevin D Cashman ◽  
Aoife Hayes ◽  
Sinead M O’Donovan ◽  
Joy Y Zhang ◽  
Michael Kinsella ◽  
...  
Keyword(s):  
Older Adults ◽  
Vitamin D3 ◽  
Dietary Calcium ◽  
Hydroxyvitamin D ◽  
25 Hydroxyvitamin D
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