Cushing’s Syndrome due to Bilateral Adrenal Macronodular Hyperplasia with Undetectable ACTH: Cell Culture of Adenoma Cells on Extracellular Matrix

1988 ◽  
Vol 29 (4) ◽  
pp. 162-167 ◽  
Author(s):  
Roger A. Cheitlin ◽  
Manfred Westphal ◽  
Claudia M. Cabrera ◽  
Dennis K. Fujii ◽  
James Snyder ◽  
...  
Keyword(s):  
Cell Culture ◽  
Extracellular Matrix ◽  
Acth Cell

scholarly journals The transcriptional landscape of a hepatoma cell line grown on scaffolds of extracellular matrix proteins

BMC Genomics ◽  
2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Souvik Ghosh ◽  
Anastasiya Börsch ◽  
Shreemoyee Ghosh ◽  
Mihaela Zavolan
Keyword(s):  
Cell Culture ◽  
Extracellular Matrix ◽  
Drug Targets ◽  
Hepatoma Cell ◽  
Growth Potential ◽  
Collagen I ◽  
Matrix Proteins ◽  
Hepatoma Cell Line ◽  
Primary Hepatocytes

Abstract Background The behavior of cells in vivo is complex and highly dynamic, as it results from an interplay between intercellular matrix proteins with surface receptors and other microenvironmental cues. Although the effects of the cellular niche have been investigated for a number of cell types using different molecular approaches, comprehensive assessments of how the global transcriptome responds to 3D scaffolds composed of various extracellular matrix (ECM) constituents at different concentrations are still lacking. Results In this study, we explored the effects of two diverse extracellular matrix (ECM) components, Collagen I and Matrigel, on the transcriptional profile of cells in a cell culture system. Culturing Huh-7 cells on traditional cell culture plates (Control) or on the ECM components at different concentrations to modulate microenvironment properties, we have generated transcriptomics data that may be further explored to understand the differentiation and growth potential of this cell type for the development of 3D cultures. Our analysis infers transcription factors that are most responsible for the transcriptome response to the extracellular cues. Conclusion Our data indicates that the Collagen I substrate induces a robust transcriptional response in the Huh-7 cells, distinct from that induced by Matrigel. Enhanced hepatocyte markers (ALB and miR-122) reveal a potentially robust remodelling towards primary hepatocytes. Our results aid in defining the appropriate culture and transcription pathways while using hepatoma cell lines. As systems mimicking the in vivo structure and function of liver cells are still being developed, our study could potentially circumvent bottlenecks of limited availability of primary hepatocytes for preclinical studies of drug targets.

A rhabdomyosarcoma hydrogel model to unveil cell-extracellular matrix interactions

2021 ◽  
Author(s):  
Mattia Saggioro ◽  
Stefania D'Agostino ◽  
Anna Gallo ◽  
Sara Crotti ◽  
Sara D'Aronco ◽  
...  
Keyword(s):  
Cell Culture ◽  
Extracellular Matrix ◽  
Three Dimensional ◽  
3D Culture ◽  
3D Cell Culture ◽  
Culture Systems ◽  
Matrix Interactions ◽  
In Vitro Systems ◽  
Extracellular Matrix Interactions

Three-dimensional (3D) culture systems are progressively getting attention given their potential in overcoming limitations of the classical 2D in vitro systems. Among different supports for 3D cell culture, hydrogels (HGs)...

scholarly journals Gene Expression Profiling of Extracellular Matrix as an Effector of Human Hepatocyte Phenotype in Primary Cell Culture

2007 ◽  
Vol 97 (2) ◽  
pp. 384-397 ◽  
Author(s):  
J. L. Page ◽  
M. C. Johnson ◽  
K. M. Olsavsky ◽  
S. C. Strom ◽  
H. Zarbl ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Culture ◽  
Extracellular Matrix ◽  
Gene Expression Profiling ◽  
Expression Profiling ◽  
Primary Cell Culture ◽  
Human Hepatocyte ◽  
Primary Cell

scholarly journals Native extracellular matrix-derived semipermeable, optically transparent, and inexpensive membrane inserts for microfluidic cell culture

Lab on a Chip ◽  
2017 ◽  
Vol 17 (18) ◽  
pp. 3146-3158 ◽  
Author(s):  
Mark J. Mondrinos ◽  
Yoon-Suk Yi ◽  
Nan-Kun Wu ◽  
Xueting Ding ◽  
Dongeun Huh
Keyword(s):  
Cell Culture ◽  
Extracellular Matrix ◽  
Optically Transparent ◽  
Microfluidic Cell Culture ◽  
New Type

This paper presents a new type of cell culture membranes engineered from native extracellular matrix (ECM) materials that are thin, semipermeable, optically transparent, and amenable to integration into microfluidic cell culture devices.

scholarly journals Development of human liver extracellular matrix hydrogel for three dimensional cell culture and cell transplantation

2017 ◽  
Vol 66 (1) ◽  
pp. S339 ◽  
Author(s):  
L. Frenguelli ◽  
W. Al-Akkad ◽  
C. Crowley ◽  
M. Tiwari ◽  
A. Mani ◽  
...  
Keyword(s):  
Cell Culture ◽  
Extracellular Matrix ◽  
Cell Transplantation ◽  
Human Liver ◽  
Three Dimensional ◽  
Dimensional Cell

scholarly journals Dynamic crosslinked and injectable biohydrogels as extracellular matrix mimics for the delivery of antibiotics and 3D cell culture

RSC Advances ◽  
2020 ◽  
Vol 10 (33) ◽  
pp. 19587-19599 ◽  
Author(s):  
Zhiping Fan ◽  
Ping Cheng ◽  
Min Liu ◽  
Sangeeta Prakash ◽  
Jun Han ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Cell Culture ◽  
Extracellular Matrix ◽  
Drug Release ◽  
Release Rate ◽  
3D Cell Culture ◽  
Drug Release Rate ◽  
Sustained Drug Delivery ◽  
Cell Scaffold ◽  
Potential Applications

Polysaccharides-polypeptide derived biohydrogels were formed using hydrazone chemistry as crosslinking strategy, which have controllable drug release rate and many other potential applications, especially in sustained drug delivery and cell scaffold.

scholarly journals Stem cell culture on polyvinyl alcohol hydrogels having different elasticity and immobilized with ECM-derived oligopeptides

2017 ◽  
Vol 37 (7) ◽  
pp. 647-660 ◽  
Author(s):  
Saradaprasan Muduli ◽  
Li-Hua Chen ◽  
Meng-Pei Li ◽  
Zhao-wen Heish ◽  
Cheng-Hui Liu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Culture ◽  
Extracellular Matrix ◽  
Stem Cell ◽  
Cell Fate ◽  
Es Cells ◽  
Embryonic Stem ◽  
Poly Vinyl Alcohol ◽  
Hematopoietic Stem ◽  
Stem Cell Culture

Abstract The physical characteristics of cell culture materials, such as their elasticity, affect stem cell fate with respect to cell proliferation and differentiation. We systematically investigated the morphologies and characteristics of several stem cell types, including human amniotic-derived stem cells, human hematopoietic stem cells, human induced pluripotent stem (iPS) cells, and embryonic stem (ES) cells on poly(vinyl alcohol) (PVA) hydrogels immobilized with and without extracellular matrix-derived oligopeptide. Human ES cells did not adhere well to soft PVA hydrogels immobilized with oligovitronectin, whereas they did adhere well to PVA hydrogel dishes with elasticities greater than 15 kPa. These results indicate that biomaterials such as PVA hydrogels should be designed to possess minimum elasticity to facilitate human ES cell attachment. PVA hydrogels immobilized with and without extracellular matrix-derived oligopeptides are excellent candidates of cell culture biomaterials for investigations into how cell culture biomaterial elasticity affects stem cell culture and differentiation.

scholarly journals One-dimensional topography underlies three-dimensional fibrillar cell migration

2009 ◽  
Vol 184 (4) ◽  
pp. 481-490 ◽  
Author(s):  
Andrew D. Doyle ◽  
Francis W. Wang ◽  
Kazue Matsumoto ◽  
Kenneth M. Yamada
Keyword(s):  
Cell Culture ◽  
Extracellular Matrix ◽  
Cell Migration ◽  
Myosin Ii ◽  
Three Dimensional ◽  
Two Dimensional ◽  
Ligand Density ◽  
One Dimensional ◽  
3D Cell Migration ◽  
Striking Contrast

Current concepts of cell migration were established in regular two-dimensional (2D) cell culture, but the roles of topography are poorly understood for cells migrating in an oriented 3D fibrillar extracellular matrix (ECM). We use a novel micropatterning technique termed microphotopatterning (μPP) to identify functions for 1D fibrillar patterns in 3D cell migration. In striking contrast to 2D, cell migration in both 1D and 3D is rapid, uniaxial, independent of ECM ligand density, and dependent on myosin II contractility and microtubules (MTs). 1D and 3D migration are also characterized by an anterior MT bundle with a posterior centrosome. We propose that cells migrate rapidly through 3D fibrillar matrices by a 1D migratory mechanism not mimicked by 2D matrices.

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