scholarly journals Native extracellular matrix-derived semipermeable, optically transparent, and inexpensive membrane inserts for microfluidic cell culture

Lab on a Chip ◽  
2017 ◽  
Vol 17 (18) ◽  
pp. 3146-3158 ◽  
Author(s):  
Mark J. Mondrinos ◽  
Yoon-Suk Yi ◽  
Nan-Kun Wu ◽  
Xueting Ding ◽  
Dongeun Huh
Keyword(s):  
Cell Culture ◽  
Extracellular Matrix ◽  
Optically Transparent ◽  
Microfluidic Cell Culture ◽  
New Type

This paper presents a new type of cell culture membranes engineered from native extracellular matrix (ECM) materials that are thin, semipermeable, optically transparent, and amenable to integration into microfluidic cell culture devices.

scholarly journals Transparent PET optical window for microfluidic cell culture device

2017 ◽  
Author(s):  
Wenfa Ng
Keyword(s):  
Thin Film ◽  
Cell Culture ◽  
Infrared Spectrum ◽  
Microfluidic Device ◽  
Infrared Imaging ◽  
Growth Chamber ◽  
Optical Window ◽  
Optically Transparent ◽  
Microfluidic Cell Culture ◽  
Imaging And Spectroscopy

Microfluidic cell culture chambers have enabled the isolation, culture and growth of various species of microorganisms and human cells. However, based on elastomers such as poly(dimethyl siloxane) (PDMS), these culture devices are not readily amenable to imaging or spectroscopy in the optical and infrared spectrum. Specifically, while PDMS is optically transparent, significant diffraction and reflection of light waves through the material does not endow it for use with various modes of spectroscopy where real-time imaging applications have been developed. For example, optical or infrared spectroscopy of the microfluidic device, through an optically transparent window, could provide readout of the types and concentration of fluorescent reporters within the growth chamber, which are usually correlated with gene expression or molecular binding events. Thus, there is a need for an optically transparent window for microfluidic cell culture device with the necessary mechanical strength, and which enables both optical and infrared imaging and spectroscopy to be performed. To this end, a flexible optically transparent polymer, poly(ethylene terephthalate), PET, could serve as the optical window of a microfluidic cell culture device. Given its optical transparency and flexibility, the PET thin film could be layered on an imaging area of a PDMS microfluidic device, that allows for optical and infrared imaging and spectroscopy. Thus, using readily available PET thin film, an optically transparent window could be placed on a PDMS microfluidic growth chamber for imaging or spectroscopy in the optical and infrared spectrum. Interested researchers may want to expand on the idea presented here.

scholarly journals Transparent PET optical window for microfluidic cell culture device

2017 ◽  
Author(s):  
Wenfa Ng
Keyword(s):  
Thin Film ◽  
Cell Culture ◽  
Infrared Spectrum ◽  
Microfluidic Device ◽  
Infrared Imaging ◽  
Growth Chamber ◽  
Optical Window ◽  
Optically Transparent ◽  
Microfluidic Cell Culture ◽  
Imaging And Spectroscopy

Microfluidic cell culture chambers have enabled the isolation, culture and growth of various species of microorganisms and human cells. However, based on elastomers such as poly(dimethyl siloxane) (PDMS), these culture devices are not readily amenable to imaging or spectroscopy in the optical and infrared spectrum. Specifically, while PDMS is optically transparent, significant diffraction and reflection of light waves through the material does not endow it for use with various modes of spectroscopy where real-time imaging applications have been developed. For example, optical or infrared spectroscopy of the microfluidic device, through an optically transparent window, could provide readout of the types and concentration of fluorescent reporters within the growth chamber, which are usually correlated with gene expression or molecular binding events. Thus, there is a need for an optically transparent window for microfluidic cell culture device with the necessary mechanical strength, and which enables both optical and infrared imaging and spectroscopy to be performed. To this end, a flexible optically transparent polymer, poly(ethylene terephthalate), PET, could serve as the optical window of a microfluidic cell culture device. Given its optical transparency and flexibility, the PET thin film could be layered on an imaging area of a PDMS microfluidic device, that allows for optical and infrared imaging and spectroscopy. Thus, using readily available PET thin film, an optically transparent window could be placed on a PDMS microfluidic growth chamber for imaging or spectroscopy in the optical and infrared spectrum. Interested researchers may want to expand on the idea presented here.

3D in vitro reconstruction of synthetic liver sinusoids in a microfluidic cell culture device

2013 ◽  
Vol 51 (01) ◽  
Author(s):  
J Böttger ◽  
J Schütte ◽  
K Benz ◽  
C Freudigmann ◽  
B Hagmeyer ◽  
...  
Keyword(s):  
Cell Culture ◽  
Liver Sinusoids ◽  
Microfluidic Cell Culture

scholarly journals The transcriptional landscape of a hepatoma cell line grown on scaffolds of extracellular matrix proteins

BMC Genomics ◽  
2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Souvik Ghosh ◽  
Anastasiya Börsch ◽  
Shreemoyee Ghosh ◽  
Mihaela Zavolan
Keyword(s):  
Cell Culture ◽  
Extracellular Matrix ◽  
Drug Targets ◽  
Hepatoma Cell ◽  
Growth Potential ◽  
Collagen I ◽  
Matrix Proteins ◽  
Hepatoma Cell Line ◽  
Primary Hepatocytes

Abstract Background The behavior of cells in vivo is complex and highly dynamic, as it results from an interplay between intercellular matrix proteins with surface receptors and other microenvironmental cues. Although the effects of the cellular niche have been investigated for a number of cell types using different molecular approaches, comprehensive assessments of how the global transcriptome responds to 3D scaffolds composed of various extracellular matrix (ECM) constituents at different concentrations are still lacking. Results In this study, we explored the effects of two diverse extracellular matrix (ECM) components, Collagen I and Matrigel, on the transcriptional profile of cells in a cell culture system. Culturing Huh-7 cells on traditional cell culture plates (Control) or on the ECM components at different concentrations to modulate microenvironment properties, we have generated transcriptomics data that may be further explored to understand the differentiation and growth potential of this cell type for the development of 3D cultures. Our analysis infers transcription factors that are most responsible for the transcriptome response to the extracellular cues. Conclusion Our data indicates that the Collagen I substrate induces a robust transcriptional response in the Huh-7 cells, distinct from that induced by Matrigel. Enhanced hepatocyte markers (ALB and miR-122) reveal a potentially robust remodelling towards primary hepatocytes. Our results aid in defining the appropriate culture and transcription pathways while using hepatoma cell lines. As systems mimicking the in vivo structure and function of liver cells are still being developed, our study could potentially circumvent bottlenecks of limited availability of primary hepatocytes for preclinical studies of drug targets.

scholarly journals Characterization and Resolution of Evaporation-Mediated Osmolality Shifts That Constrain Microfluidic Cell Culture in Poly(dimethylsiloxane) Devices

2007 ◽  
Vol 79 (3) ◽  
pp. 1126-1134 ◽  
Author(s):  
Yun Seok Heo ◽  
Lourdes M. Cabrera ◽  
Jonathan W. Song ◽  
Nobuyuki Futai ◽  
Yi-Chung Tung ◽  
...  
Keyword(s):  
Cell Culture ◽  
Microfluidic Cell Culture

A rhabdomyosarcoma hydrogel model to unveil cell-extracellular matrix interactions

2021 ◽  
Author(s):  
Mattia Saggioro ◽  
Stefania D'Agostino ◽  
Anna Gallo ◽  
Sara Crotti ◽  
Sara D'Aronco ◽  
...  
Keyword(s):  
Cell Culture ◽  
Extracellular Matrix ◽  
Three Dimensional ◽  
3D Culture ◽  
3D Cell Culture ◽  
Culture Systems ◽  
Matrix Interactions ◽  
In Vitro Systems ◽  
Extracellular Matrix Interactions

Three-dimensional (3D) culture systems are progressively getting attention given their potential in overcoming limitations of the classical 2D in vitro systems. Among different supports for 3D cell culture, hydrogels (HGs)...

scholarly journals Gene Expression Profiling of Extracellular Matrix as an Effector of Human Hepatocyte Phenotype in Primary Cell Culture

2007 ◽  
Vol 97 (2) ◽  
pp. 384-397 ◽  
Author(s):  
J. L. Page ◽  
M. C. Johnson ◽  
K. M. Olsavsky ◽  
S. C. Strom ◽  
H. Zarbl ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Culture ◽  
Extracellular Matrix ◽  
Gene Expression Profiling ◽  
Expression Profiling ◽  
Primary Cell Culture ◽  
Human Hepatocyte ◽  
Primary Cell
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