Characterization of the Microcirculation during Ischemia and Reperfusion in the Penumbra of a Rat Model of Temporary Middle Cerebral Artery Occlusion: A Laser Doppler Flowmetry Study

1994 ◽  
Vol 14 (5) ◽  
pp. 289-295 ◽  
Author(s):  
T.B. Müller ◽  
O. Haraldseth ◽  
G. Unsgård
Keyword(s):  
Middle Cerebral Artery ◽  
Middle Cerebral Artery Occlusion ◽  
Cerebral Artery ◽  
Laser Doppler Flowmetry ◽  
Rat Model ◽  
Artery Occlusion ◽  
Ischemia And Reperfusion ◽  
Doppler Flowmetry ◽  
Cerebral Artery Occlusion

Reliability of behavioral tests in the middle cerebral artery occlusion model of rat

2018 ◽  
Vol 53 (5) ◽  
pp. 478-490 ◽  
Author(s):  
Junghoon Yu ◽  
Jinkyoo Moon ◽  
Joonyoung Jang ◽  
Jee In Choi ◽  
Jooeun Jung ◽  
...  
Keyword(s):  
Middle Cerebral Artery ◽  
Middle Cerebral Artery Occlusion ◽  
Cerebral Artery ◽  
Rat Model ◽  
Artery Occlusion ◽  
Behavioral Tests ◽  
Walking Test ◽  
Satisfactory Level ◽  
Cylinder Test ◽  
Cerebral Artery Occlusion

Stroke is one of the leading causes of death and disability worldwide, and its incidence is increasing. To overcome impairment from stroke, translational research for developing new therapeutic technologies has been conducted and middle cerebral artery occlusion (MCAo) in rat is the representative model. Since recovery from neurological impairment in contralateral limbs caused by brain damage is the major goal of treatment, behavioral tests that assess the relevant function are used. To determine therapeutic effect, obtaining reliable results of behavioral assessment is a prerequisite. However, studies on the reliability of behavioral tests in the MCAo rat model and necessity of prior training have not yet been reported. In this study, the authors investigate relative and absolute inter-rater reliabilities of modified neurological severity score (mNSS), cylinder test, and grid-walking test before training and repeated training every week until the reliability of results reached a satisfactory level. The training included repeated learning of the scoring system and decreasing disagreements among the raters. For MCAo modeling, adult male Sprague-Dawley rats were subjected to 90 min of transient MCAo. Six raters conducted behavioral tests via observation of video-recording on sham-operated and MCAo model rats at 3 or 7 days after the intervention. An independent experimenter randomly numbered each video clip to blind the experiment. The results of reliabilities were unacceptable before training and improved to a satisfactory level after 6 weeks of training in all of the tests. In conclusion, mNSS, cylinder test, and grid-walking test on the MCAo rat model are reliable evaluation methods after conducting appropriate training.

Sign in / Sign up

Export Citation Format

Share Document