scholarly journals Endothelin-1 Induced Middle Cerebral Artery Occlusion Model for Ischemic Stroke with Laser Doppler Flowmetry Guidance in Rat

10.3791/50014 ◽  
2013 ◽  
Author(s):  
Saeed Ansari ◽  
Hassan Azari ◽  
Kenneth J. Caldwell ◽  
Robert W. Regenhardt ◽  
Vishnumurthy S. Hedna ◽  
...  
Keyword(s):  
Ischemic Stroke ◽  
Middle Cerebral Artery ◽  
Middle Cerebral Artery Occlusion ◽  
Cerebral Artery ◽  
Laser Doppler Flowmetry ◽  
Artery Occlusion ◽  
Doppler Flowmetry ◽  
Endothelin 1 ◽  
Occlusion Model ◽  
Cerebral Artery Occlusion

scholarly journals Effects of Noninvasive Facial Nerve Stimulation in the Dog Middle Cerebral Artery Occlusion Model of Ischemic Stroke

Stroke ◽  
2014 ◽  
Vol 45 (4) ◽  
pp. 1102-1107 ◽  
Author(s):  
Mark K. Borsody ◽  
Chisa Yamada ◽  
Dawn Bielawski ◽  
Tamara Heaton ◽  
Fernando Castro Prado ◽  
...  
Keyword(s):  
Ischemic Stroke ◽  
Facial Nerve ◽  
Middle Cerebral Artery ◽  
Middle Cerebral Artery Occlusion ◽  
Cerebral Artery ◽  
Nerve Stimulation ◽  
Artery Occlusion ◽  
Occlusion Model ◽  
Facial Nerve Stimulation ◽  
Cerebral Artery Occlusion

scholarly journals Middle Cerebral Artery Occlusion Model in Rodents: Methods and Potential Pitfalls

2011 ◽  
Vol 2011 ◽  
pp. 1-9 ◽  
Author(s):  
Fudong Liu ◽  
Louise D. McCullough
Keyword(s):  
Ischemic Stroke ◽  
Middle Cerebral Artery ◽  
Middle Cerebral Artery Occlusion ◽  
Cerebral Artery ◽  
Artery Occlusion ◽  
Stroke Model ◽  
Occlusion Model ◽  
Cerebral Artery Occlusion ◽  
Paradoxical Results ◽  
Age And Sex

A variety of animal models have been developed for modeling ischemic stroke. The middle cerebral artery occlusion (MCAO) model has been utilized extensively, especially in rodents. While the MCAO model provides stroke researchers with an excellent platform to investigate the disease, controversial or even paradoxical results are occasionally seen in the literature utilizing this model. Various factors exert important effects on the outcome in this stroke model, including the age and sex of the animal examined. This paper discusses emerging information on the effects of age and sex on ischemic outcomes after MCAO, with an emphasis on mouse models of stroke.

Enhanced protection by melatonin and meloxicam combination in a middle cerebral artery occlusion model of acute ischemic stroke in rat

2002 ◽  
Vol 80 (3) ◽  
pp. 210-217 ◽  
Author(s):  
Y K Gupta ◽  
Geeta Chaudhary ◽  
Kusum Sinha
Keyword(s):  
Ischemic Stroke ◽  
Middle Cerebral Artery ◽  
Acute Ischemic Stroke ◽  
Middle Cerebral Artery Occlusion ◽  
Cerebral Artery ◽  
Motor Performance ◽  
Artery Occlusion ◽  
Occlusion Model ◽  
Grip Test ◽  
Cerebral Artery Occlusion

Mixed efficacy of neuroprotective drugs in clinical trials has led to the emergence of the approach of combination therapy in stroke. The present study was carried out to investigate the effect of the combination of melatonin (potent antioxidant) and meloxicam (preferential inhibitor of cyclooxygenase-2 enzyme) against a middle cerebral artery occlusion model of stroke in rats. Male Wistar rats in the weight range of 250–300 g were used. Rats were anesthetized using chloral hydrate (400 mg/kg i.p) and subjected to 2 h of transient middle cerebral artery occlusion. Melatonin was administered at a dose of 20 mg/kg i.p. four times: at the time of middle cerebral artery occlusion, 1.5 h after middle cerebral artery occlusion, at the time of reperfusion, and 1 h after reperfusion. Meloxicam (2.5 mg/kg) was administered 4 h after middle cerebral artery occlusion. Motor performance tests (grip test, foot fault test, rotarod performance test, spontaneous locomotor activity), markers of oxidative stress, and triphenyltetrazolium chloride staining were carried out 24 h after middle cerebral artery occlusion. A vehicle-treated group was run in parallel. It was observed that melatonin treatment improved the motor performance and significantly attenuated the levels of malondialdehyde (MDA) as compared with the middle cerebral artery occluded group. Meloxicam treatment at the dose used neither showed significant improvement on the motor performance nor decreased the levels of MDA significantly as compared with the middle cerebral artery occluded group. However, when the combination of the two drugs was used, better protection was observed as was evident by the significant decrease in the percent foot fault errors, the increase in the time spent on the rotarod, and the increase in the six-point neurological score and grip test score. There was also a significant decrease in the levels of MDA in the combination group. The results of the present study demonstrate that enhanced protection is observed with the use of a combination of melatonin plus meloxicam in the middle cerebral artery occlusion model of acute ischemic stroke in rats.Key words: melatonin, antioxidant, meloxicam, focal ischemia.

The Pyk2/MCU pathway in the rat middle cerebral artery occlusion model of ischemic stroke

2018 ◽  
Vol 131 ◽  
pp. 52-62 ◽  
Author(s):  
Kun Zhang ◽  
Jiajia Yan ◽  
Liang Wang ◽  
Xinying Tian ◽  
Tong Zhang ◽  
...  
Keyword(s):  
Ischemic Stroke ◽  
Middle Cerebral Artery ◽  
Middle Cerebral Artery Occlusion ◽  
Cerebral Artery ◽  
Artery Occlusion ◽  
Occlusion Model ◽  
Cerebral Artery Occlusion
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