α-Tocopherol and Phospholipase A2 in Liver and Brain of Chicks Posthatching: The Influence of Dietary Fat and Vitamin E

1995 ◽  
Vol 39 (5) ◽  
pp. 302-309 ◽  
Author(s):  
Herbert Fuhrmann ◽  
Hans Peter Sallmann
Keyword(s):  
Vitamin E ◽  
Dietary Fat ◽  
Alpha Tocopherol ◽  
Phospholipase A

THE EFFECT OF UNSATURATION OF DIETARY FAT AND OF ANTIOXIDANTS ON THE DEVELOPMENT OF LIVER DAMAGE

1959 ◽  
Vol 37 (1) ◽  
pp. 493-499 ◽  
Author(s):  
L. S. Valberg ◽  
Rose A. Young ◽  
J. M. R. Beveridge
Keyword(s):  
Amino Acids ◽  
Fatty Acids ◽  
Antioxidant Activity ◽  
Vitamin E ◽  
Liver Damage ◽  
Dietary Fat ◽  
Unsaturated Fatty Acids ◽  
Alpha Tocopherol ◽  
Liver Necrosis ◽  
Gamma Tocopherol

The addition of unsaturated fatty acids, even in small amounts, to diets low in vitamin E, selenium, and the sulphur-containing amino acids greatly accelerated the development of acute liver necrosis in rats. The production of this lesion was shown also to be affected in a similar manner by the nature of the dietary fat in the ration consumed immediately prior to the removal of protective substances such as the sulphur-containing amino acids; unsaturated fatty acids or lipid again acted as predisposing factors.Gamma-tocopherol incorporated in the diet at a level of 0.02% and injected at a level of 1.5 mg/rat/day was equally as effective as alpha-tocopherol in preventing liver necrosis. The protective effect of gamma-tocopherol against the development of liver necrosis lends support to the thesis that alpha-tocopherol protects against liver damage by virtue of its antioxidant activity.

THE EFFECT OF UNSATURATION OF DIETARY FAT AND OF ANTIOXIDANTS ON THE DEVELOPMENT OF LIVER DAMAGE

1959 ◽  
Vol 37 (3) ◽  
pp. 493-499 ◽  
Author(s):  
L. S. Valberg ◽  
Rose A. Young ◽  
J. M. R. Beveridge
Keyword(s):  
Amino Acids ◽  
Fatty Acids ◽  
Antioxidant Activity ◽  
Vitamin E ◽  
Liver Damage ◽  
Dietary Fat ◽  
Unsaturated Fatty Acids ◽  
Alpha Tocopherol ◽  
Liver Necrosis ◽  
Gamma Tocopherol

The addition of unsaturated fatty acids, even in small amounts, to diets low in vitamin E, selenium, and the sulphur-containing amino acids greatly accelerated the development of acute liver necrosis in rats. The production of this lesion was shown also to be affected in a similar manner by the nature of the dietary fat in the ration consumed immediately prior to the removal of protective substances such as the sulphur-containing amino acids; unsaturated fatty acids or lipid again acted as predisposing factors.Gamma-tocopherol incorporated in the diet at a level of 0.02% and injected at a level of 1.5 mg/rat/day was equally as effective as alpha-tocopherol in preventing liver necrosis. The protective effect of gamma-tocopherol against the development of liver necrosis lends support to the thesis that alpha-tocopherol protects against liver damage by virtue of its antioxidant activity.

Alpha Tocopherol Loaded in Liposome: Preparation, Optimization, Characterization and Sperm Motility Protection

2020 ◽  
Vol 10 (3) ◽  
pp. 228-236 ◽  
Author(s):  
Lamia Taouzinet ◽  
Sofiane Fatmi ◽  
Allaeddine Khellouf ◽  
Mohamed Skiba ◽  
Mokrane Iguer-ouada
Keyword(s):  
Vitamin E ◽  
Sperm Motility ◽  
High Performance ◽  
Positive Impact ◽  
Alpha Tocopherol ◽  
Ethanol Injection ◽  
Liposome Preparation ◽  
Liposome Size ◽  
Optimum Solution ◽  
The Impact

Background: Alpha-tocopherol is a potent antioxidant involved in sperm protection particularly during cryopreservation. However, its poor solubility limits the optimal protection in aqueous solutions. Objective: The aim of this study was to enhance the solubility of α-tocopherol by the use of liposomes. Methods: The experimental approach consisted to load vitamin E in liposomes prepared by ethanol injection method and the optimization carried out by an experimental design. The optimum solution was characterized by high performance liquid chromatography and scanning electron microscope. Finely, the impact on sperm motility protection was studied by the freezing technic of bovine sperm. Results: The optimum solution was obtained when using 10.9 mg/ml of phospholipids, 1.7 mg/ml of cholesterol and 2 mg/ml of vitamin E. The liposome size was 99.86 nm, providing 78.47% of loaded efficiency. The results showed also a significant positive impact on sperm motility after hours of preservation. Conclusion: In conclusion, the current results showed the interest of liposome preparation as an alternative to enhance vitamin E solubility and to protect spermatozoa during cryopreservation.

scholarly journals Effect of Dietary Fat Quality and Vitamin E on the Antioxidant Potential of Pigs

1983 ◽  
Vol 24 (2) ◽  
pp. 135-147 ◽  
Author(s):  
P. Thode Jensen ◽  
H. E. Nielsen ◽  
V. Danielsen ◽  
T. Leth
Keyword(s):  
Vitamin E ◽  
Dietary Fat ◽  
Antioxidant Potential ◽  
Fat Quality

scholarly journals Alpha-Tocopherol Metabolites (The Vitamin E Metabolome) and Their Interindividual Variability during Supplementation

Antioxidants ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 173
Author(s):  
Desirée Bartolini ◽  
Rita Marinelli ◽  
Danilo Giusepponi ◽  
Roberta Galarini ◽  
Carolina Barola ◽  
...  
Keyword(s):  
Vitamin E ◽  
Target Genes ◽  
Interindividual Variability ◽  
Pregnane X Receptor ◽  
Alpha Tocopherol ◽  
Therapeutic Interventions ◽  
Intrinsic Variability ◽  
Blood Leukocytes ◽  
Definition Of ◽  
And Function

The metabolism of α-tocopherol (α-TOH, vitamin E) shows marked interindividual variability, which may influence the response to nutritional and therapeutic interventions with this vitamin. Recently, new metabolomics protocols have fostered the possibility to explore such variability for the different metabolites of α-TOH so far identified in human blood, i.e., the “vitamin E metabolome”, some of which have been reported to promote important biological functions. Such advances prompt the definition of reference values and degree of interindividual variability for these metabolites at different levels of α-TOH intake. To this end, a one-week oral administration protocol with 800 U RRR-α-TOH/day was performed in 17 healthy volunteers, and α-TOH metabolites were measured in plasma before and at the end of the intervention utilizing a recently validated LC-MS/MS procedure; the expression of two target genes of α-TOH with possible a role in the metabolism and function of this vitamin, namely pregnane X receptor (PXR) and the isoform 4F2 of cytochrome P450 (CYP4F2) was assessed by immunoblot in peripheral blood leukocytes. The levels of enzymatic metabolites showed marked interindividual variability that characteristically increased upon supplementation. With the exception of α-CEHC (carboxy-ethyl-hydroxychroman) and the long-chain metabolites M1 and α-13′OH, such variability was found to interfere with the possibility to utilize them as sensitive indicators of α-TOH intake. On the contrary, the free radical-derived metabolite α-tocopheryl quinone significantly correlated with the post-supplementation levels of α-TOH. The supplementation stimulated PXR, but not CYP4F2, expression of leucocytes, and significant correlations were observed between the baseline levels of α-TOH and both the baseline and post-supplementation levels of PXR. These findings provide original analytical and molecular information regarding the human metabolism of α-TOH and its intrinsic variability, which is worth considering in future nutrigenomics and interventions studies.

Combined effects of vitamin E (alpha-tocopherol) and cisplatin on the growth of murine neuroblastoma in vivo

1988 ◽  
Vol 24 (11) ◽  
pp. 1751-1758 ◽  
Author(s):  
Kohji Sue ◽  
Akira Nakagawara ◽  
Shin-Ichi Okuzono ◽  
Takahiko Fukushige ◽  
Keiichi Ikeda
Keyword(s):  
Vitamin E ◽  
Alpha Tocopherol ◽  
Combined Effects ◽  
Murine Neuroblastoma

Cerebrospinal fluid levels of alpha-tocopherol (vitamin E) in Alzheimer's disease

1997 ◽  
Vol 104 (6-7) ◽  
pp. 703-710 ◽  
Author(s):  
F. J. Jiménez-Jiménez ◽  
F. de Bustos ◽  
J. A. Molina ◽  
J. Benito-León ◽  
A. Tallón-Barranco ◽  
...  
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Cerebrospinal Fluid ◽  
Vitamin E ◽  
Alpha Tocopherol ◽  
Cerebrospinal Fluid Levels ◽  
Fluid Levels

Phospholipase A 2 mRNA in remnant kidney is modulated by dietary alpha-tocopherol

1999 ◽  
Vol 3 (4) ◽  
pp. 250-253
Author(s):  
G. Dorsam ◽  
N. B. Kuemmerle ◽  
R. J. Krieg ◽  
R. C. Franson ◽  
J. C. M. Chan
Keyword(s):  
Alpha Tocopherol ◽  
Phospholipase A ◽  
Remnant Kidney ◽  
Phospholipase A 2

Simultaneous quantification of vitamin E and vitamin E metabolites in equine plasma and serum using LC-MS/MS

2021 ◽  
pp. 104063872110054
Author(s):  
Hadi Habib ◽  
Carrie J. Finno ◽  
Ingrid Gennity ◽  
Gianna Favro ◽  
Erin Hales ◽  
...  
Keyword(s):  
Vitamin E ◽  
Normal Growth ◽  
Alpha Tocopherol ◽  
Limits Of Detection ◽  
Phase Gradient ◽  
Equine Plasma ◽  
Accuracy And Precision ◽  
Low Concentrations ◽  
Liquid Chromatography Tandem Mass ◽  
Acquity Uplc

Vitamin E deficiencies can impact normal growth and development in humans and animals, and assessment of circulating levels of vitamin E and its metabolites may be an important endpoint for evaluation. Development of a sensitive method to detect and quantify low concentrations of vitamin E and metabolites in biological specimens allows for a proper diagnosis for patients and animals that are deficient. We developed a method to simultaneously extract, detect, and quantify the vitamin E compounds alpha-tocopherol (α-TP), gamma-tocopherol (γ-TP), alpha-tocotrienol (α-TT), and gamma-tocotrienol (γ-TT), and the corresponding metabolites formed after β-oxidation of α-TP and γ-TP, alpha-carboxymethylbutyl hydroxychroman (α-CMBHC) and alpha- or gamma-carboxyethyl hydroxychroman (α- or γ-CEHC), respectively, from equine plasma and serum. Quantification was achieved through liquid chromatography–tandem mass spectrometry. We applied a 96-well high-throughput format using a Phenomenex Phree plate to analyze plasma and serum. Compounds were separated by using a Waters ACQUITY UPLC BEH C18 column with a reverse-phase gradient. The limits of detection for the metabolites and vitamin E compounds were 8–330 pg/mL. To validate the method, intra-day and inter-day accuracy and precision were evaluated along with limits of detection and quantification. The method was then applied to determine concentrations of these analytes in plasma and serum of horses. Alpha-TP levels were 3–6 µg/mL of matrix; the metabolites were found at much lower levels, 0.2–1.0 ng/mL of matrix.

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