Arginine Vasopressin and Oxytocin Increase Intracellular Calcium and cAMP in Human Glomerular Epithelial Cells in Culture

1996 ◽  
Vol 19 (2) ◽  
pp. 81-86 ◽  
Author(s):  
Michael Späth ◽  
Hermann Pavenstädt ◽  
Jan Petersen ◽  
Peter Schollmeyer
Keyword(s):  
Epithelial Cells ◽  
Intracellular Calcium ◽  
Arginine Vasopressin ◽  
Cells In Culture ◽  
Glomerular Epithelial Cells

Identification of a Receptor for Interleukin 1 (IL-1) on Human Glomerular Epithelial Cells in Culture

1993 ◽  
Vol 3 (2) ◽  
pp. 111-116 ◽  
Author(s):  
Gertrud Maria Hänsch ◽  
Monica Braunger ◽  
Klaus Möhring ◽  
Christof Wagner
Keyword(s):  
Epithelial Cells ◽  
Interleukin 1 ◽  
Cells In Culture ◽  
Glomerular Epithelial Cells

An ultrastructural study of rat glomerular epithelial cells in culture

1994 ◽  
Vol 27 (1) ◽  
pp. 49-53 ◽  
Author(s):  
Tamaki Sasaki ◽  
Hitoshi Tamai ◽  
Yoshiyuki Jyo ◽  
Gengo Osawa
Keyword(s):  
Epithelial Cells ◽  
Ultrastructural Study ◽  
Cells In Culture ◽  
Glomerular Epithelial Cells

Unique Phosphorylation of Vimentin Filaments in Glomerular Epithelial Cells in Culture and in Disease

Nephron ◽  
1997 ◽  
Vol 77 (4) ◽  
pp. 373-377 ◽  
Author(s):  
Ryuji Nagasawa ◽  
Koichi Kanozawa ◽  
Tetsuya Mitarai ◽  
Masaki Inagaki ◽  
Naoki Maruyama
Keyword(s):  
Epithelial Cells ◽  
Cells In Culture ◽  
Glomerular Epithelial Cells ◽  
Vimentin Filaments

scholarly journals Effects of arginine- and lysine-vasopressin on phospholipase C activity, intracellular calcium concentration and prostaglandin F2alpha secretion in pig endometrial cells

Reproduction ◽  
2001 ◽  
pp. 605-612 ◽  
Author(s):  
GT Braileanu ◽  
SM Simasko ◽  
J Hu ◽  
A Assiri ◽  
MA Mirando
Keyword(s):  
Epithelial Cells ◽  
Intracellular Calcium ◽  
Phospholipase C ◽  
Arginine Vasopressin ◽  
Stromal Cells ◽  
Calcium Concentration ◽  
Intracellular Calcium Concentration ◽  
Endometrial Cells ◽  
Lysine Vasopressin ◽  
Luminal Epithelial Cells

Oxytocin and vasopressin are related peptides that have receptors in the uterus. Species from families other than Suidae produce only arginine-vasopressin; in contrast, pigs apparently express both arginine- and lysine-vasopressin. The aim of this study was to determine whether arginine- or lysine-vasopressin would activate phospholipase C, increase intracellular calcium concentration [Ca(2+)](i) and stimulate PGF(2alpha) production in enriched cultures of stromal, glandular epithelial and luminal epithelial cells from pig endometrium. Cells were obtained from gilts on day 16 after oestrus by differential enzymatic digestion and sieve separation. After 96 h in culture, the cells were treated with 0 or 100 nmol arginine- or lysine-vasopressin l(-1). The responses to 100 nmol oxytocin l(-1) and 100 nmol GnRH l(-1) were used as positive and negative controls, respectively. Consistent with previous results, oxytocin stimulated phospholipase C activity (P < 0.05), increased [Ca(2+)](i) (P < 0.05) and promoted PGF(2alpha) secretion (P < 0.05) from stromal and glandular epithelial cells. Activity of phospholipase C, [Ca(2+)](i) and PGF(2alpha) release were also increased (P < 0.05) by arginine-vasopressin in stromal cells, but the responses were less (P < 0.01) than those induced by oxytocin. An oxytocin antagonist attenuated the [Ca(2+)](i) response of stromal cells to both oxytocin and arginine-vasopressin. Sequential treatment of cells with oxytocin and arginine-vasopressin indicated that oxytocin desensitized the response to oxytocin, but arginine-vasopressin did not similarly desensitize the response to oxytocin. In glandular and luminal epithelial cells, arginine-vasopressin did not stimulate phospholipase C activity, [Ca(2+)](i) or PGF(2alpha) secretion. Neither GnRH nor lysine-vasopressin induced phospholipase C activity, increased [Ca(2+)](i) or stimulated PGF(2alpha) production in any endometrial cell type. These results indicate that oxytocin receptors can bind arginine-vasopressin more readily than they bind lysine-vasopressin. Type 1 vasopressin receptors may also exist in endometrium predominantly on cells other than stromal, glandular epithelial and luminal epithelial cells, as in previous studies both arginine-vasopressin and lysine-vasopressin stimulated phospholipase C activity in endometrial explants to a similar extent as oxytocin.

scholarly journals Interleukin‐1β is an autocrine growth factor of rat glomerular epithelial cells in culture

2001 ◽  
Vol 16 (6) ◽  
pp. 1149-1155 ◽  
Author(s):  
Fumiko Tateyama ◽  
Hideaki Yamabe ◽  
Hiroshi Osawa ◽  
Mitsuaki Kaizuka ◽  
Kenichi Shirato ◽  
...  
Keyword(s):  
Growth Factor ◽  
Epithelial Cells ◽  
Interleukin 1Β ◽  
Autocrine Growth ◽  
Autocrine Growth Factor ◽  
Cells In Culture ◽  
Glomerular Epithelial Cells

scholarly journals Urokinase synthesis and binding by glomerular epithelial cells in culture

1989 ◽  
Vol 36 (4) ◽  
pp. 593-600 ◽  
Author(s):  
Eric Rondeau ◽  
Satochi Ochi ◽  
Roger Lacave ◽  
Ci-Jiang He ◽  
Robert Medcalf ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Cells In Culture ◽  
Glomerular Epithelial Cells

Partial characterization of proteoglycans synthesized by human glomerular epithelial cells in culture

1990 ◽  
Vol 277 (2) ◽  
pp. 389-401 ◽  
Author(s):  
David J. Klein ◽  
Theodore R. Oegema ◽  
Thomas S. Fredeen ◽  
Fokko van der Woude ◽  
Youngki Kim ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Partial Characterization ◽  
Cells In Culture ◽  
Glomerular Epithelial Cells
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