Both subtype 1 and 2 receptors of angiotensin II participate in regulation of intracellular calcium in glomerular epithelial cells

2001 ◽  
Vol 138 (1) ◽  
pp. 40-49 ◽  
Author(s):  
Ram Sharma ◽  
Mukut Sharma ◽  
Susanne Vamos ◽  
Virginia J. Savin ◽  
Thomas B. Wiegmann
Keyword(s):  
Angiotensin Ii ◽  
Epithelial Cells ◽  
Intracellular Calcium ◽  
Glomerular Epithelial Cells

Documentation of angiotensin II receptors in glomerular epithelial cells

1998 ◽  
Vol 274 (3) ◽  
pp. F623-F627 ◽  
Author(s):  
Mukut Sharma ◽  
Ram Sharma ◽  
Andrew. S. Greene ◽  
Ellen T. McCarthy ◽  
Virginia J. Savin
Keyword(s):  
Signal Transduction ◽  
Angiotensin Ii ◽  
Epithelial Cells ◽  
Glomerular Filtration ◽  
Mesangial Cells ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Angiotensin Ii Receptors ◽  
Filtration Barrier ◽  
Glomerular Epithelial Cells

Angiotensin II decreases glomerular filtration rate, renal plasma flow, and glomerular capillary hydraulic conductivity. Although angiotensin II receptors have been demonstrated in mesangial cells and proximal tubule cells, the presence of angiotensin II receptors in glomerular epithelial cells has not previously been shown. Previously, we have reported that angiotensin II caused an accumulation of cAMP and a reorganization of the actin cytoskeleton in cultured glomerular epithelial cells. Current studies were conducted to verify the presence of angiotensin II receptors by immunological and non-peptide receptor ligand binding techniques and to ascertain the activation of intracellular signal transduction in glomerular epithelial cells in response to angiotensin II. Confluent monolayer cultures of glomerular epithelial cells were incubated with angiotensin II, with or without losartan and/or PD-123,319 in the medium. Membrane vesicle preparations were obtained by homogenization of washed cells followed by centrifugation. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of membrane proteins followed by multiscreen immunoblotting was used to determine the presence of angiotensin II receptor type 1 (AT1) or type 2 (AT2). Angiotensin II-mediated signal transduction in glomerular epithelial cells was studied by measuring the levels of cAMP, using radioimmunoassay. Results obtained in these experiments showed the presence of both AT1 and AT2 receptor types in glomerular epithelial cells. Angiotensin II was found to cause an accumulation of cAMP in glomerular epithelial cells, which could be prevented only by simultaneous use of losartan and PD-123,319, antagonists for AT1 and AT2, respectively. The presence of both AT1 and AT2 receptors and an increase in cAMP indicate that glomerular epithelial cells respond to angiotensin II in a manner distinct from that of mesangial cells or proximal tubular epithelial cells. Our results suggest that glomerular epithelial cells participate in angiotensin II-mediated control of the glomerular filtration barrier.

scholarly journals Changes in glomerular epithelial cells induced by FGF2 and FGF2 neutralizing antibody in puromycin aminonucleoside nephropathy

1997 ◽  
Vol 51 (1) ◽  
pp. 301-309 ◽  
Author(s):  
Tamaki Sasaki ◽  
Yoshiyuki Jyo ◽  
Nobuya Tanda ◽  
Yasuhiko Kawakami ◽  
Tsutomu Nohno ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Neutralizing Antibody ◽  
Puromycin Aminonucleoside ◽  
Glomerular Epithelial Cells

scholarly journals RAGE ligands stimulate angiotensin II type I receptor (AT1) via RAGE/AT1 complex on the cell membrane

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Serina Yokoyama ◽  
Tatsuo Kawai ◽  
Koichi Yamamoto ◽  
Huang Yibin ◽  
Hiroko Yamamoto ◽  
...  
Keyword(s):  
Angiotensin Ii ◽  
Cardiovascular Diseases ◽  
Epithelial Cells ◽  
G Protein ◽  
Rat Kidney ◽  
Chinese Hamster ◽  
Cellular Responses ◽  
Type I ◽  
Α Subunit ◽  
Mesenchymal Transition

AbstractThe receptor for advanced glycation end-products (RAGE) and the G protein-coupled angiotensin II (AngII) type I receptor (AT1) play a central role in cardiovascular diseases. It was recently reported that RAGE modifies AngII-mediated AT1 activation via the membrane oligomeric complex of the two receptors. In this study, we investigated the presence of the different directional crosstalk in this phenomenon, that is, the RAGE/AT1 complex plays a role in the signal transduction pathway of RAGE ligands. We generated Chinese hamster ovary (CHO) cells stably expressing RAGE and AT1, mutated AT1, or AT2 receptor. The activation of two types of G protein α-subunit, Gq and Gi, was estimated through the accumulation of inositol monophosphate and the inhibition of forskolin-induced cAMP production, respectively. Rat kidney epithelial cells were used to assess RAGE ligand-induced cellular responses. We determined that RAGE ligands activated Gi, but not Gq, only in cells expressing RAGE and wildtype AT1. The activation was inhibited by an AT1 blocker (ARB) as well as a RAGE inhibitor. ARBs inhibited RAGE ligand-induced ERK phosphorylation, NF-κB activation, and epithelial–mesenchymal transition of rat renal epithelial cells. Our findings suggest that the activation of AT1 plays a central role in RAGE-mediated cellular responses and elucidate the role of a novel molecular mechanism in the development of cardiovascular diseases.

scholarly journals In vitro incubation and study of kidney glomerular epithelial cells

1979 ◽  
Vol 15 (1) ◽  
pp. 80-87 ◽  
Author(s):  
Peter M. Andrews ◽  
Marguerite Stauver
Keyword(s):  
Epithelial Cells ◽  
In Vitro Incubation ◽  
Glomerular Epithelial Cells

Angiotensin II Evokes Calcium-Mediated Signaling Events in Isolated Dog Pancreatic Epithelial Cells

Pancreas ◽  
2002 ◽  
Vol 25 (3) ◽  
pp. 290-295 ◽  
Author(s):  
Aaron S. Fink ◽  
Yuanhong Wang ◽  
Tatiana Mendez ◽  
Roger T. Worrell ◽  
Douglas Eaton ◽  
...  
Keyword(s):  
Angiotensin Ii ◽  
Epithelial Cells ◽  
Signaling Events

scholarly journals An Intracellular Calcium Signal Activates p70 but Not p90 Ribosomal S6 Kinase in Liver Epithelial Cells

1997 ◽  
Vol 272 (3) ◽  
pp. 1920-1928 ◽  
Author(s):  
Lee M. Graves ◽  
Yaqin He ◽  
John Lambert ◽  
Deborah Hunter ◽  
Xiong Li ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Intracellular Calcium ◽  
Calcium Signal ◽  
S6 Kinase ◽  
Liver Epithelial Cells ◽  
P90 Ribosomal S6 Kinase ◽  
Ribosomal S6 Kinase
Sign in / Sign up

Export Citation Format

Share Document