Imaging Uremic Red Blood Cells with the Atomic Force Microscope

1994 ◽  
Vol 14 (3) ◽  
pp. 197-200 ◽  
Author(s):  
Pierre Zachée ◽  
Marc Boogaerts ◽  
Johan Snauwaert ◽  
Louis Hellemans
Keyword(s):  
Red Blood Cells ◽  
Atomic Force Microscope ◽  
Blood Cells ◽  
Atomic Force

In Situ Observation of Freeze Fractured Red Blood Cells with a High-Vacuum Low-Temperature Atomic Force Microscope

2004 ◽  
Vol 10 (S02) ◽  
pp. 1098-1099
Author(s):  
Charles B Mooney ◽  
Keiichi Nakamoto ◽  
Shin-ichi Kitamura
Keyword(s):  
Low Temperature ◽  
Red Blood Cells ◽  
Atomic Force Microscope ◽  
Blood Cells ◽  
High Vacuum ◽  
In Situ Observation ◽  
Atomic Force

Extended abstract of a paper presented at Microscopy and Microanalysis 2004 in Savannah, Georgia, USA, August 1–5, 2004.

Imaging red blood cells with the atomic force microscope

1996 ◽  
Vol 95 (3) ◽  
pp. 472-481 ◽  
Author(s):  
Pierre Zachée ◽  
Johan Snauwaert ◽  
Peter Vandenberghe ◽  
Louis Hellemans ◽  
Marc Boogaerts
Keyword(s):  
Red Blood Cells ◽  
Atomic Force Microscope ◽  
Blood Cells ◽  
Atomic Force

scholarly journals Affinity Imaging of Red Blood Cells Using an Atomic Force Microscope

2000 ◽  
Vol 48 (5) ◽  
pp. 719-724 ◽  
Author(s):  
Michel Grandbois ◽  
Wolfgang Dettmann ◽  
Martin Benoit ◽  
Hermann E. Gaub
Keyword(s):  
Red Blood Cells ◽  
Atomic Force Microscope ◽  
Blood Cells ◽  
Helix Pomatia ◽  
Image Formation ◽  
Rupture Force ◽  
Receptor Ligand ◽  
Agarose Beads ◽  
Atomic Force ◽  
Group A

SUMMARY We used an atomic force microscope (AFM) to produce an image of a mixed layer of group A and O red blood cells with a contrast based only on the measured strength of a specific receptor-ligand pair. The image was obtained by measuring and plotting for each image pixel the adhesion force between the mixed RBC layer and the AFM tip functionalized with Helix pomatia lectin. The high specificity of that lectin for the N-acetylgalactosamine-terminated glycolipids present in the membrane of group A RBCs enabled us to discriminate between the two cell populations and to produce an image based on affinity contrast. The rupture force of the adhesion events leading to the image formation were quantitatively analyzed and compared to rupture forces measured with the same AFM tip on N-acetylgalactosamine tethered to agarose beads. The mean rupture force was found to be 65 pN when measured on the group A RBCs and 35 pN on the agarose beads. These results show that the adhesion, mediated by only a few receptor-ligand pairs, produces sufficient contrast for the affinity image formation.

Study on the Young’s Modulus of Red Blood Cells Using Atomic Force Microscope

2014 ◽  
Vol 627 ◽  
pp. 197-201 ◽  
Author(s):  
Cheng Chang Lien ◽  
Meng Chien Wu ◽  
Chyung Ay
Keyword(s):  
Red Blood Cells ◽  
Atomic Force Microscope ◽  
Young’S Modulus ◽  
Blood Cells ◽  
Young's Modulus ◽  
Maximum Load ◽  
The Other ◽  
Atomic Force ◽  
The Relationship ◽  
Force Displacement

The force-displacement curves of rat’s red blood cells (RBC) were measured by atomic force microscope (AFM) in this study, and the young’s modulus of RBC were calculated. The different speed and loads of probe on AFM was conducted to exam the effect of young’s modulus in RBC. Furthermore, the relationship between young’s modulus of RBC and different depth of indentation from force-displacement curves were investigated. The experimental results and analysis showed that when probe’s maximum load was 5 nN and the velocity was set for 1, 5, 10 and 20 μm/s, the young’s modulus of normal red blood cells for probe down measurements to AFM were 129.56 ± 42.80, 141.56 ± 31.15, 147.90 ± 24.35 and 149.69 ± 29.27 kPa, respectively. It represented that the young’s modulus of normal red blood cells depended on probe’s velocity. Then when probe’s velocity was 1 μm/s and the load was changed to 1, 5 and 10 nN, the young’s modulus of normal red blood cells were measured for 41.45 ± 22.64, 82.72 ± 53.99 and 202.40 ± 16.01 kPa, respectively. It represented that the young’s modulus of normal red blood cells depended on the probe’s load. On the other side, the results of force-displacement curves exam demonstrated that the deeper of probe indented in cells, the measured young’s modulus of normal red blood cells would be increased more.

scholarly journals The Quality Assessment of Stored Red Blood Cells Probed Using Atomic-Force Microscopy

2014 ◽  
Vol 2014 ◽  
pp. 1-5 ◽  
Author(s):  
I. M. Lamzin ◽  
R. M. Khayrullin
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Morphological Characteristics ◽  
High Increase ◽  
Mean Values ◽  
Force Microscopy ◽  
Atomic Force ◽  
The Mean ◽  
The Moment

At the moment the suitability of stored red blood cells (sRBC) for transfusion is checked by routine methods such as haemoglobin estimation and the level of haemolysis. These methods cannot characterize directly the quality of the membranes of sRBC. The aim of this work is to assess the quality of sRBC based on such criteria as the membrane’s stiffness and the size and the form of sRBC. Materials and Methods. We have investigated 5 series of dry cytosmears of the sRBC which had been kept in blood bank in a period from 1 to 35 days. After AFM imaging, in every specimen, 5 RBC were chosen at random; the diameter, the height, and the stiffness were measured on each of them. Results. The present study shows high increase of the mean values of YM and height of RBC after 35 days of storage and decrease of the mean values of their diameter. Conclusion. Statistically significant high increase of the mean values of YM indicates the decrease of the elasticity of the cells in the course of storing of the RBC. This parameter along with the morphological characteristics can be used as criterion for assessment of applicability of the sRBC for blood transfusion.

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