Modulatory Role of Endothelium-Derived Relaxing Factors on the Response to Vasoconstrictors and Flow-Pressure Curve in the Isolated Perfused Rat Kidney

1996 ◽  
Vol 33 (2) ◽  
pp. 119-123 ◽  
Author(s):  
Félix Vargas ◽  
Antonio Osuna
Keyword(s):  
Rat Kidney ◽  
Pressure Curve ◽  
Isolated Perfused Rat Kidney ◽  
Perfused Rat Kidney ◽  
Flow Pressure

Nitric oxide enhancement of erythropoietin production in the isolated perfused rat kidney

1995 ◽  
Vol 269 (4) ◽  
pp. C917-C922 ◽  
Author(s):  
K. Yoshioka ◽  
J. W. Fisher
Keyword(s):  
Nitric Oxide ◽  
Rat Kidney ◽  
Mrna Levels ◽  
Isolated Perfused Rat Kidney ◽  
Intracellular Cgmp ◽  
Perfused Rat Kidney ◽  
Arterial Po2

We have previously reported that nitric oxide (NO) and guanosine 3',5'-cyclic monophosphate (cGMP) may be involved in the regulation of erythropoietin (Epo) production in response to hypoxia both in vivo and in vitro (20). In the present studies, we have used the isolated perfused rat kidney to assess the role of NO in oxygen sensing and Epo production. When arterial PO2 was reduced from 100 mmHg (normoxemic) to 30 mmHg (hypoxemic) in the perfusate of this system, perfusate levels of Epo were significantly increased. This hypoxia-induced increase in Epo production was significantly decreased by the addition of NG-nitro-L-arginine methyl ester (L-NAME; 1 mM) to the perfusates. Hypoxemic perfusion also produced a significant increase, and L-NAME significantly inhibited this increase, in intracellular cGMP levels in the kidney when compared with normoxemic perfused kidneys. Quantitative reverse transcription-polymerase chain reaction also revealed that hypoxemic perfusion produced significant increases in Epo mRNA levels in the kidney, which was blocked by L-NAME. Our findings further support an important role for the NO/cGMP system in hypoxic regulation of Epo production.

scholarly journals The critical role of adenosine and guanosine in the affinity of dinucleoside polyphosphates to P2X -receptors in the isolated perfused rat kidney

2001 ◽  
Vol 132 (2) ◽  
pp. 467-474 ◽  
Author(s):  
Markus Van Der Giet ◽  
Tim Westhoff ◽  
Okan Cinkilic ◽  
Joachim Jankowski ◽  
Hartmut Schlüter ◽  
...  
Keyword(s):  
Rat Kidney ◽  
Critical Role ◽  
P2x Receptors ◽  
Isolated Perfused Rat Kidney ◽  
Perfused Rat Kidney ◽  
Dinucleoside Polyphosphates

scholarly journals Serine synthesis by an isolated perfused rat kidney preparation

1985 ◽  
Vol 230 (2) ◽  
pp. 303-311 ◽  
Author(s):  
R C Scaduto ◽  
E J Davis
Keyword(s):  
Aspartate Aminotransferase ◽  
Essential Role ◽  
Rat Kidney ◽  
Glucose Production ◽  
Complete Inhibition ◽  
Maximal Rate ◽  
Isolated Perfused Rat Kidney ◽  
Perfused Rat Kidney ◽  
Glucose Synthesis

The isolated perfused rat kidney was shown to synthesize serine from aspartate or glutamate, both of which are also precursors of glucose. The major products of aspartate metabolism were ammonia, serine, glutamate, glucose, glutamine and CO2. Perfusion of kidneys with aspartate in the presence of amino-oxyacetate resulted in a near-complete inhibition of aspartate metabolism, illustrating the essential role of aspartate aminotransferase in the metabolism of this substrate. Radioactivity from 14C-labelled aspartate and from 14C-labelled glycerol was incorporated into serine and glucose. Production of both glucose and serine from aspartate was suppressed in the presence of 3-mercaptopicolinic acid. These data provide evidence for the operation of the phosphorylated and/or non-phosphorylated pathway for serine production to the presence of 3-mercaptopicolinic acid. This is explained by simultaneous glycolysis. The rate of glucose production, but not that of serine, was greater in kidneys perfused with glutamate or with aspartate plus glycerol than the rates obtained by perfusion with aspartate alone. These data are taken to suggest that serine synthesis occurred at a near-maximal rate, and that the capacity of the kidney for serine synthesis from glucose precursors is lower than that for glucose synthesis.

Role of cyclic AMP in the regulation of renin release from the isolated perfused rat kidney

1978 ◽  
Vol 56 (S1) ◽  
pp. 51-59 ◽  
Author(s):  
K. G. Hofbauer ◽  
A. Konrads ◽  
K. Schwarz ◽  
U. Werner
Keyword(s):  
Cyclic Amp ◽  
Rat Kidney ◽  
Renin Release ◽  
Isolated Perfused Rat Kidney ◽  
Perfused Rat Kidney

Validation of a toxicity testing model by evaluating oxygen supply and energy state in the isolated perfused rat kidney

1991 ◽  
Vol 25 (3) ◽  
pp. 195-204 ◽  
Author(s):  
Takano Takehito ◽  
Nakata Kazuyo ◽  
Kawakami Tsuyoshi ◽  
Miyazaki Yoshifumi ◽  
Murakami Masataka ◽  
...  
Keyword(s):  
Oxygen Supply ◽  
Energy State ◽  
Rat Kidney ◽  
Toxicity Testing ◽  
Testing Model ◽  
Isolated Perfused Rat Kidney ◽  
Perfused Rat Kidney

Aldosterone Effects on Renal Function in the Isolated Perfused Rat Kidney

1979 ◽  
Vol 2 (1) ◽  
pp. 1-11
Author(s):  
Richard Solomon ◽  
Patricio Silva ◽  
Franklin H. Epstein
Keyword(s):  
Renal Function ◽  
Rat Kidney ◽  
Isolated Perfused Rat Kidney ◽  
Perfused Rat Kidney
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