Abnormal Biochemistry of Subcellular Membranes Isolated from Nonvascular Smooth Muscles of Spontaneously Hypertensive Rats

1982 ◽  
Vol 19 (6) ◽  
pp. 273-283 ◽  
Author(s):  
C.Y. Kwan ◽  
A.K. Grover ◽  
Y. Sakai
Keyword(s):  
Spontaneously Hypertensive Rats ◽  
Smooth Muscles ◽  
Hypertensive Rats ◽  
Spontaneously Hypertensive

scholarly journals Features of ionic transport processes in a model of arterial hypertension

2018 ◽  
Vol 17 (4) ◽  
pp. 103-109
Author(s):  
A. V. Nosarev ◽  
Yu. G. Birulina ◽  
I. V. Petrova ◽  
I. V. Kovalev ◽  
S. V. Gusakova ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Erythrocyte Membrane ◽  
Spontaneously Hypertensive Rats ◽  
Smooth Muscles ◽  
Calcium Ionophore ◽  
Transport Processes ◽  
Ionophore A23187 ◽  
Hypertensive Rats ◽  
Spontaneously Hypertensive ◽  
K Channels

Aim.To study the ionic mechanisms which underlie regulation contractile activity of vascular smooth muscles and the erythrocytes membrane potential from spontaneously hypertensive rats.Materials and methods.The effect of acetylcholine (ACX), 10 μM, and Ca2+ -ionophore A23187, 10 μM, on the contractile reactions of isolated smooth muscle segments of the aorta from 11-week-old spontaneously hypertensive rats (SHR) and Wistar – Kyoto rats (WKY) induced by phenylephrine (PE), 1 μM, was measured by the mechanography. 11-week-old SHR were divided into control and experimental groups depending on the drug administration (amlodipine 10 mg/kg given intragastrically). Blood pressure (BP) in SHR was measured before and after treatment. The amplitude of the hyperpolarizating response (HO) and the activity of Ca2+-dependent K+-channels of the erythrocyte membrane of SHR were performed with potentiometric method.Results.In response to the stimulating effect of the ACX or the calcium ionophore A23187, the smooth muscle segments of the aorta from the WKY and SHR precontracted with PE were relaxed. To the addition of the ACX, the vascular segments of the SHR responded with a stronger dilatation than the WKY segments, but not the action of the Ca2+-ionophore. Treatment of SHR with blocker of Ca2+-channels of L-type amlodipine caused a decrease the BP, and an increase in the relaxing effect of intact aortic segments on the ACX and Ca2+-ionophore. There was an increase the amplitude of HO and activity of Ca2+-dependent K+-channels of the erythrocyte membrane from SHR.Conclusion.Relaxation of vascular smooth muscle in SHR is caused by changes in the endothelial function and Ca2+-dependent ionic conductivity of the myocyte membrane. An increase of the hyperpolarizating response of the erythrocyte membrane from rats with spontaneous hypertension is associated with activation of potassium channels. The selective effect on these intracellular targets can serve as a basis for the development of antihypertensive drugs.

scholarly journals Effect of protamine sulfate on the isolated mesenteric arteries of normotensive and spontaneously hypertensive rats

2008 ◽  
Vol 60 (2) ◽  
pp. 163-168 ◽  
Author(s):  
Zorana Orescanin-Dusic ◽  
S. Milovanovic ◽  
M. Spasic ◽  
R. Radojicic ◽  
D. Blagojevic
Keyword(s):  
Significant Role ◽  
Spontaneously Hypertensive Rats ◽  
Smooth Muscles ◽  
Relaxation Effect ◽  
Protamine Sulfate ◽  
Mesenteric Arteries ◽  
Vascular Smooth Muscles ◽  
Hypertensive Rats ◽  
Relaxant Effect ◽  
Spontaneously Hypertensive

We tested the relaxant effect of increased protamine sulfate (PS) amounts (10, 20, 50, 100 and 150 ?g/ml) on the isolated mesenteric arteries of normotensive and spontaneously hypertensive (SH) rats, with or without endothelium. PS caused concentration-dependent relaxation of isolated mesenteric arteries in both types of rats. The relaxation effect of PS was lower in SH rats than in normotensive ones. Our results indicate that the vascular smooth muscles play a significant role in PS-mediated relaxation.

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