Subcloning and Restriction Enzyme Mapping of Bovine Adenovirus Type 2

Intervirology ◽  
1993 ◽  
Vol 36 (2) ◽  
pp. 72-78 ◽  
Author(s):  
K. Salmon ◽  
L. Esford ◽  
Y. Haj-Ahmad
Keyword(s):  
Restriction Enzyme ◽  
Adenovirus Type ◽  
Bovine Adenovirus ◽  
Restriction Enzyme Mapping ◽  
Enzyme Mapping ◽  
Adenovirus Type 2

DNA structure, and hemagglutination properties of bovine adenovirus type 2 strains which bypass species specificity

1983 ◽  
Vol 77 (2-4) ◽  
pp. 181-194 ◽  
Author(s):  
S. Bel�k ◽  
Gy. Berencsi ◽  
M. Rusvay ◽  
K. Luk�cs ◽  
I. N�sz
Keyword(s):  
Dna Structure ◽  
Adenovirus Type ◽  
Species Specificity ◽  
Bovine Adenovirus ◽  
Adenovirus Type 2

Sequence Analysis of the Putative E3 Region of Bovine Adenovirus Type 2

Intervirology ◽  
1994 ◽  
Vol 37 (5) ◽  
pp. 277-286
Author(s):  
L.E. Esford ◽  
Y. Haj-Ahmad
Keyword(s):  
Dna Sequences ◽  
Human Adenovirus ◽  
Adenovirus Type ◽  
Open Reading Frames ◽  
Bovine Adenovirus ◽  
Adenovirus Type 5 ◽  
E3 Region ◽  
Adenovirus Type 2

Adenoviruses are nonenveloped icosahedral-shaped particles. The double-stranded viral DNA genome contains four major early transcription units, designated El (a and b), E2 (a and b), E3 and E4, which are expressed in a regulated manner soon after infection. The gene products of the region E3, shown to be nonessential for viral replication in vitro, are believed to be involved in counteracting host immunosurveillance. Human adenovirus type 5 DNA sequences of transcription units L4 and L5 adjacent to E3 were used to localize E3 within the bovine adenovirus type 2. The DNA sequences between 74.8 and 84.4 mu containing E3 and the fiber gene were determined. The E3 region was found to consist of about 2.3 kb pairs and to encode four proteins longer than 60 amino acids. However, these four open reading frames did not show significant homology to any other known adenovirus DNA or protein sequence.

First Isolation, Molecular Characterization, and Serological Survey of Bovine Adenovirus Type 2 in Japan

2021 ◽  
Author(s):  
Naoki Kubota ◽  
Sho Orihara ◽  
Asuka Kumagai ◽  
Kumiko Kimura ◽  
Shinichi Hatama
Keyword(s):  
Respiratory Disease ◽  
Electron Microscopic Observation ◽  
Adenovirus Type ◽  
Biological Properties ◽  
Electron Microscopic ◽  
Bovine Adenovirus ◽  
Enveloped Virus ◽  
Infected Cells ◽  
Adenovirus Type 2

Abstract Bovine adenovirus type 2 (BAdV-2), a member of the Mastadenovirus genus of the Adenoviridae family, is involved in mild respiratory disease and is rarely isolated from cattle in the world. Here, we isolated BAdV-2 for the first time in Japan, from the feces of cattle with severe respiratory symptoms. The isolate, designated strain KY19-1, was identified morphologically and genetically. Electron microscopic observation of infected cells showed intranuclear, non-enveloped virus particles, approximately 70 nm in diameter and with typical adenoviral morphology. Whole-genome sequencing revealed that the viral genome is 33,175 bp long, with 105 bp inverted terminal repeats and encoding 32 predicted genes. KY19-1 has a similar genomic organization to the prototype BAdV-2 strain No. 19 with overall 99.1% nucleotide identity. Notable differences were found in the E3 region of the genome, which may affect various biological properties, including pathogenicity. The seroepidemiology of BAdV-2 was also investigated, using a virus-neutralization test, and 92.8 % of the Japanese cattle (n=1,325) were seropositive, with the positivity rate increased by age. Further experimental infections with KY19-1 may elucidate the pathogenic properties of BAdV-2 and the importance of this virus as a causal agent of bovine respiratory disease.

Subtypes of bovine adenovirus type 2 exhibit major differences in region E3

Virology ◽  
1986 ◽  
Vol 153 (2) ◽  
pp. 262-271 ◽  
Author(s):  
S. Belák ◽  
A. Virtanen ◽  
J. Zabielski ◽  
M. Rusvai ◽  
G. Berencsi ◽  
...  
Keyword(s):  
Adenovirus Type ◽  
Bovine Adenovirus ◽  
Adenovirus Type 2

scholarly journals Epidemic of Bovine Adenovirus type 2 Associated with Respiratory and Digestive Signs in Dairy Cattle

2020 ◽  
Vol 73 (10) ◽  
pp. 577-581
Author(s):  
Naoki KUBOTA ◽  
Sho ORIHARA ◽  
Takuhiro KIRA ◽  
Shinichi HATAMA
Keyword(s):  
Dairy Cattle ◽  
Adenovirus Type ◽  
Bovine Adenovirus ◽  
Adenovirus Type 2

Cloning and sequencing of the bovine adenovirus type 2 early region 4

Gene ◽  
1997 ◽  
Vol 185 (2) ◽  
pp. 181-186 ◽  
Author(s):  
Linda Fitzgerald ◽  
Masud Ansari ◽  
Yousef Haj-Ahmad
Keyword(s):  
Adenovirus Type ◽  
Cloning And Sequencing ◽  
Bovine Adenovirus ◽  
Early Region ◽  
Early Region 4 ◽  
Adenovirus Type 2
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