Sequence Analysis of the Putative E3 Region of Bovine Adenovirus Type 2

Intervirology ◽  
1994 ◽  
Vol 37 (5) ◽  
pp. 277-286
Author(s):  
L.E. Esford ◽  
Y. Haj-Ahmad
Keyword(s):  
Dna Sequences ◽  
Human Adenovirus ◽  
Adenovirus Type ◽  
Open Reading Frames ◽  
Bovine Adenovirus ◽  
Adenovirus Type 5 ◽  
E3 Region ◽  
Adenovirus Type 2

Adenoviruses are nonenveloped icosahedral-shaped particles. The double-stranded viral DNA genome contains four major early transcription units, designated El (a and b), E2 (a and b), E3 and E4, which are expressed in a regulated manner soon after infection. The gene products of the region E3, shown to be nonessential for viral replication in vitro, are believed to be involved in counteracting host immunosurveillance. Human adenovirus type 5 DNA sequences of transcription units L4 and L5 adjacent to E3 were used to localize E3 within the bovine adenovirus type 2. The DNA sequences between 74.8 and 84.4 mu containing E3 and the fiber gene were determined. The E3 region was found to consist of about 2.3 kb pairs and to encode four proteins longer than 60 amino acids. However, these four open reading frames did not show significant homology to any other known adenovirus DNA or protein sequence.

Viral DNA Sequences in Cells Transformed by Simian Virus 40, Adenovirus Type 2 and Adenovirus Type 5

1974 ◽  
Vol 39 (0) ◽  
pp. 615-632 ◽  
Author(s):  
J. Sambrook ◽  
M. Botchan ◽  
P. Gallimore ◽  
B. Ozanne ◽  
U. Pettersson ◽  
...  
Keyword(s):  
Dna Sequences ◽  
Simian Virus 40 ◽  
Adenovirus Type ◽  
Simian Virus ◽  
Viral Dna ◽  
Adenovirus Type 5 ◽  
Adenovirus Type 2 ◽  
In Cells

In vitro functional genetic modification of canine adenovirus type 2 genome by CRISPR/Cas9

2021 ◽  
Author(s):  
Abdul Mohin Sajib ◽  
Payal Agarwal ◽  
Daniel J. Patton ◽  
Rebecca L. Nance ◽  
Natalie A. Stahr ◽  
...  
Keyword(s):  
Genetic Modification ◽  
Adenovirus Type ◽  
Adenovirus Type 2

scholarly journals In Vitro and In Vivo Evaluation of Human Adenovirus Type 49 as a Vector for Therapeutic Applications

Viruses ◽  
2021 ◽  
Vol 13 (8) ◽  
pp. 1483
Author(s):  
Emily A. Bates ◽  
John R. Counsell ◽  
Sophie Alizert ◽  
Alexander T. Baker ◽  
Natalie Suff ◽  
...  
Keyword(s):  
Viral Vector ◽  
Ex Vivo ◽  
Human Adenovirus ◽  
Adenovirus Type ◽  
Cell Types ◽  
In Vivo Evaluation ◽  
In Vivo Biodistribution ◽  
Adenovirus Type 5

The human adenovirus phylogenetic tree is split across seven species (A–G). Species D adenoviruses offer potential advantages for gene therapy applications, with low rates of pre-existing immunity detected across screened populations. However, many aspects of the basic virology of species D—such as their cellular tropism, receptor usage, and in vivo biodistribution profile—remain unknown. Here, we have characterized human adenovirus type 49 (HAdV-D49)—a relatively understudied species D member. We report that HAdV-D49 does not appear to use a single pathway to gain cell entry, but appears able to interact with various surface molecules for entry. As such, HAdV-D49 can transduce a broad range of cell types in vitro, with variable engagement of blood coagulation FX. Interestingly, when comparing in vivo biodistribution to adenovirus type 5, HAdV-D49 vectors show reduced liver targeting, whilst maintaining transduction of lung and spleen. Overall, this presents HAdV-D49 as a robust viral vector platform for ex vivo manipulation of human cells, and for in vivo applications where the therapeutic goal is to target the lung or gain access to immune cells in the spleen, whilst avoiding liver interactions, such as intravascular vaccine applications.

Transcription of adenovirus type 2 genes in a cell-free system: apparent heterogeneity of initiation at some promoters

1981 ◽  
Vol 1 (7) ◽  
pp. 635-651
Author(s):  
D C Lee ◽  
R G Roeder
Keyword(s):  
Adenovirus Type ◽  
Free System ◽  
Ribonucleic Acids ◽  
Cell Free System ◽  
Cell Extracts ◽  
A Cell ◽  
Adenovirus Type 2

We examined the transcription of a variety of adenovirus type 2 genes in a cell-free system containing purified ribonucleic acid polymerase II and a crude extract from cultured human cells. The early EIA, EIB, EIII, and EIV genes and the intermediate polypeptide IX gene, all of which contain a recognizable TATAA sequence upstream from the cap site, were actively transcribed in vitro, albeit with apparently different efficiencies, whereas the early EII (map position 74.9) and IVa2 genes, both of which lack a TATAA sequence, were not actively transcribed. A reverse transcriptase-primer extension analysis showed that the 5' ends of the in vitro transcripts were identical to those of the corresponding in vivo ribonucleic acids and that, in those instances where initiation was heterogeneous in vivo, a similar kind of heterogeneity was observed in the cell-free system. Transcription of the polypeptide IX gene indicated that this transcript was not terminated at, or processed to, the polyadenylic acid addition site in vitro. We also failed to observe, using the in vitro system, any indication of transcriptional regulation based on the use of adenovirus type 2-infected cell extracts.

scholarly journals Comparative optical properties of free and assembled hexon capsomeres of human adenovirus type 2

FEBS Letters ◽  
1978 ◽  
Vol 85 (1) ◽  
pp. 52-56 ◽  
Author(s):  
Pierre A. Boulanger ◽  
Christiane Devaux ◽  
Marie H. Loucheux-Lefebvre
Keyword(s):  
Optical Properties ◽  
Human Adenovirus ◽  
Adenovirus Type ◽  
Adenovirus Type 2

scholarly journals Isolation and Phenotypic Characterization of Human Adenovirus Type 2 Temperature-Sensitive Mutants

1978 ◽  
Vol 41 (2) ◽  
pp. 303-314 ◽  
Author(s):  
G. R. Martin ◽  
R. Warocquier ◽  
C. Cousin ◽  
J.-C. D'Halluin ◽  
P. A. Boulanger
Keyword(s):  
Human Adenovirus ◽  
Adenovirus Type ◽  
Phenotypic Characterization ◽  
Temperature Sensitive ◽  
Temperature Sensitive Mutants ◽  
Adenovirus Type 2
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