scholarly journals Cl Transport in Complemented CF Bronchial Epithelial Cells Correlates with CFTR mRNA Expression Levels

2008 ◽  
Vol 22 (1-4) ◽  
pp. 057-068 ◽  
Author(s):  
Beate Illek ◽  
Rosalie Maurisse ◽  
Logan Wahler ◽  
Karl Kunzelmann ◽  
Horst Fischer ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Mrna Expression ◽  
Bronchial Epithelial Cells ◽  
Bronchial Epithelial ◽  
Expression Levels ◽  
Cl Transport ◽  
Mrna Expression Levels

scholarly journals Loss of sphingosine 1-phosphate receptor 3 gene function impairs injury-induced stromal angiogenesis in mouse cornea

2020 ◽  
Author(s):  
Shingo Yasuda ◽  
Takayoshi Sumioka ◽  
Hiroki Iwanishi ◽  
Yuka Okada ◽  
Masayasu Miyajima ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Growth Factor ◽  
Epithelial Cells ◽  
Mrna Expression ◽  
Vascular Endothelial ◽  
Corneal Epithelial Cells ◽  
Corneal Epithelial ◽  
Expression Levels ◽  
Sphingosine 1 Phosphate ◽  
Mrna Expression Levels

AbstractSphingosine 1-phosphate (S1P) is a bioactive sphingolipid generated through sphingosine kinase1 (SPK1)-mediated phosphorylation of sphingosine. We show here that injury-induced S1P upregulation increases corneal neovascularization through stimulating S1PR3, a cognate receptor. since this response was suppressed in S1PR3-knockout mice. Furthermore, Cayman10444, a selective S1PR3 inhibitor, reduced this response in WT mice. Such reductions in neovascularization were associated with reduced vascular endothelial growth factor A (VEGF-A) mRNA expression levels in WT TKE2 corneal epithelial cells and macrophages treated with CAY10444 as well as macrophages isolated from S1PR3 KO mice. S1P increased tube-like vessel formation in human vascular endothelial cells (HUVEC) and human retinal microvascular endothelial cells (HRMECs) cells expressing S1PR3. In S1PR3 KO mice, TGFβ1-induced increases in αSMA gene expression levels were suppressed relative to those in the WT counterparts. In S1PR3 deficient macrophages, VEGF-A expression levels were lower than in WT macrophages. Transforming growth factor β1(TGFβ1) upregulated SPK1 expression levels in ocular fibroblasts and TKE2 corneal epithelial cells. CAY10444 blocked S1P-induced increases in VEGF-A mRNA expression levels in TKE2 corneal epithelial cells. Endogenous S1P signaling upregulated VEGF-A and VE-cadherin mRNA expression levels in HUVEC. Unlike in TKE2 cells, SIS3 failed to block TGFβ1-induced VEGF-A upregulation in ocular fibroblasts. Taken together, these results indicate that injury-induced TGFβ1 upregulation increases S1P generation through increases in SPK1 activity. The rise in S1P formation stimulates the S1PR3-linked signaling pathway, which in turn increases VEGF-A expression levels and angiogenesis in mouse corneas.

scholarly journals Accelerated epithelial cell senescence in IPF and the inhibitory role of SIRT6 in TGF-β-induced senescence of human bronchial epithelial cells

2011 ◽  
Vol 300 (3) ◽  
pp. L391-L401 ◽  
Author(s):  
Shunsuke Minagawa ◽  
Jun Araya ◽  
Takanori Numata ◽  
Satoko Nojiri ◽  
Hiromichi Hara ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Cellular Senescence ◽  
Type I Collagen ◽  
Transforming Growth Factor ◽  
Pathological Finding ◽  
Bronchial Epithelial Cells ◽  
Type I ◽  
Human Bronchial Epithelial Cells ◽  
Bronchial Epithelial ◽  
Expression Levels

Reepithelialization of remodeled air spaces with bronchial epithelial cells is a prominent pathological finding in idiopathic pulmonary fibrosis (IPF) and is implicated in IPF pathogenesis. Recent studies suggest that epithelial senescence is a risk factor for development of IPF, indicating such reepithelialization may be influenced by the acceleration of cellular senescence. Among the sirtuin (SIRT) family, SIRT6, a class III histone deacetylase, has been demonstrated to antagonize senescence. We evaluated the senescence of bronchiolization in association with SIRT6 expression in IPF lung. Senescence-associated β-galactosidase staining and immunohistochemical detection of p21 were performed to evaluate cellular senescence. As a model for transforming growth factor (TGF)-β-induced senescence of abnormal reepithelialization, we used primary human bronchial epithelial cells (HBEC). The changes of SIRT6, p21, and interleukin (IL)-1β expression levels in HBEC, as well as type I collagen expression levels in fibroblasts, were evaluated. In IPF lung samples, an increase in markers of senescence and SIRT6 expression was found in the bronchial epithelial cells lining cystically remodeled air spaces. We found that TGF-β induced senescence in primary HBEC by increasing p21 expression, and, whereas TGF-β also induced SIRT6, it was not sufficient to inhibit cellular senescence. However, overexpression of SIRT6 efficiently inhibited TGF-β-induced senescence via proteasomal degradation of p21. TGF-β-induced senescent HBEC secreted increased amounts of IL-1β, which was sufficient to induce myofibroblast differentiation in fibroblasts. These findings suggest that accelerated epithelial senescence plays a role in IPF pathogenesis through perpetuating abnormal epithelial-mesenchymal interactions, which can be antagonized by SIRT6.

CCR3 mRNA Expression in Bronchial Epithelial Cells and Various Cells in Allergic Inflammation

1999 ◽  
Vol 120 (1) ◽  
pp. 45-47 ◽  
Author(s):  
Hajime Oyamada ◽  
Yumiko Kamada ◽  
Tomoe Kuwasaki ◽  
Yoshiyuki Yamada ◽  
Yoshimi Kobayashi ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Mrna Expression ◽  
Allergic Inflammation ◽  
Bronchial Epithelial Cells ◽  
Bronchial Epithelial

Aspergillus Fumigatus Extract Suppresses the mRNA Expression of the IP-10 in Human Bronchial Epithelial Cells

2014 ◽  
Vol 151 (1_suppl) ◽  
pp. P248-P248
Author(s):  
Bharat Bhushan ◽  
James E. Norton ◽  
Dave Gupta ◽  
Quen Sha ◽  
James W. Schroeder ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Aspergillus Fumigatus ◽  
Mrna Expression ◽  
Bronchial Epithelial Cells ◽  
Human Bronchial Epithelial Cells ◽  
Bronchial Epithelial

MUC1 Mucin mRNA Expression in Cultured Human Nasal and Bronchial Epithelial Cells

1992 ◽  
Vol 6 (5) ◽  
pp. 516-520 ◽  
Author(s):  
Michael A. Hollingsworth ◽  
Surinder K. Batra ◽  
Wen-Ning Qi ◽  
James R. Yankaskas
Keyword(s):  
Epithelial Cells ◽  
Mrna Expression ◽  
Bronchial Epithelial Cells ◽  
Bronchial Epithelial ◽  
Muc1 Mucin

83 BONE MORPHOGENETIC PROTEIN SIGNALING DURING INTERACTION OF THE BOVINE EMBRYO WITH OVIDUCTAL EPITHELIAL CELLS IN VITRO

2017 ◽  
Vol 29 (1) ◽  
pp. 149
Author(s):  
E. V. García ◽  
M. Hamdi ◽  
A. D. Barrera ◽  
M. J. Sánchez-Calabuig ◽  
A. Gutiérrez-Adán ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Mrna Expression ◽  
Relative Abundance ◽  
Bovine Embryo ◽  
Cell Stage ◽  
Expression Levels ◽  
Bmp Receptors ◽  
Bmp Signalling ◽  
Mrna Expression Levels

In previous studies, we have demonstrated that different signalling components of bone morphogenetic proteins (BMP) are expressed in an anatomically and temporally regulated fashion in the bovine oviduct. However, a local response of this signalling to the embryo presence has not been elucidated yet. The aim of the present study was to evaluate whether the interaction of the embryo with the oviduct can induce changes in the gene expression of BMP signalling components. For this purpose, we used an in vitro co-culture system of a bovine oviducal epithelial cell (BOEC) monolayer with pre-implantation embryos in 2 developmental time points: before and during the main phase of embryonic genome activation (EGA). Isthmus epithelial cells from post-ovulatory stage oviducts (Day 2–4) were cultured in 500 μL of SOF + 10% FCS in 4-well plates at 38.5°C, 5% CO2, 5% O2, and 90% N2. On Day 6 of culture, medium was replaced with SOF + 5% FCS, and 24 h later BOEC monolayer was cultured in the absence or presence of in vitro-produced embryos from 2- to 8-cell stage [G1 BOEC; 33–54 h post-insemination (hpi)] or from 8- to 16-cell stage (G2 BOEC; 54–98 hpi) in the same conditions. In both groups, a polyester mesh was used to define a local co-culture area, and 30 embryos per well were placed in a 6 × 5 grid over the monolayer. In addition, as control groups, embryos in both developmental stages were cultured either in SOF + 5% FCS (G1 FCS and G2 FCS) or in SOF + 3 mg mL−1 BSA (G1 BSA and G2 BSA). At 54 hpi (G1 BOEC/BSA/FCS) or 98 hpi (G2 BOEC/BSA/FCS), embryos that reached 8- or 16-cell stage, respectively, were transferred to SOF + BSA and cultured until Day 9. The mRNA expression levels of 3 BMP receptors (BMPRIA/IB/II), 2 signalling proteins (SMAD1/5), 1 inhibitor (SMAD6), and 1 target gene (ID2) were analysed by qPCR in 5 samples of BOEC cultured with or without embryos before or during EGA, and in 3 pools of 10 embryos at 8 (54 hpi), 16 (98 hpi), and blastocyst stage (Day 7–8) from all groups. Genes H2A.Z and ACTG1 were used as housekeeping genes, and statistical differences were assessed by ANOVA. The presence of the embryo, irrespective the stage, significantly reduced the expression levels of BMPRIB, BMPRII, SMAD1, SMAD6, and ID2 in BOEC. Embryos that interacted with BOEC before EGA (G1 BOEC) showed a significant increase in the relative abundance of SMAD1 at the 8-cell stage compared with controls. Moreover, embryos that interacted with BOEC during EGA (G2 BOEC) showed a significant increase in the relative abundance of BMPRIB, BMPRII, and ID2 at the 16-cell stage when compared with controls. However, no differences were observed in the mRNA expression levels of BMP signalling components in the blastocysts between groups. In conclusion, local embryo-oviduct interaction in vitro induces changes in the transcriptional levels of BMP signalling, causing a bidirectional response that reduces the expression levels of this signalling in the oviducal cells while increases them in the embryo at early stages. This suggests that BMP signalling pathway could be involved in an early cross-talk between the bovine embryo and the oviduct during first stages of development.

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