CCR3 mRNA Expression in Bronchial Epithelial Cells and Various Cells in Allergic Inflammation

1999 ◽  
Vol 120 (1) ◽  
pp. 45-47 ◽  
Author(s):  
Hajime Oyamada ◽  
Yumiko Kamada ◽  
Tomoe Kuwasaki ◽  
Yoshiyuki Yamada ◽  
Yoshimi Kobayashi ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Mrna Expression ◽  
Allergic Inflammation ◽  
Bronchial Epithelial Cells ◽  
Bronchial Epithelial

Aspergillus Fumigatus Extract Suppresses the mRNA Expression of the IP-10 in Human Bronchial Epithelial Cells

2014 ◽  
Vol 151 (1_suppl) ◽  
pp. P248-P248
Author(s):  
Bharat Bhushan ◽  
James E. Norton ◽  
Dave Gupta ◽  
Quen Sha ◽  
James W. Schroeder ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Aspergillus Fumigatus ◽  
Mrna Expression ◽  
Bronchial Epithelial Cells ◽  
Human Bronchial Epithelial Cells ◽  
Bronchial Epithelial

MUC1 Mucin mRNA Expression in Cultured Human Nasal and Bronchial Epithelial Cells

1992 ◽  
Vol 6 (5) ◽  
pp. 516-520 ◽  
Author(s):  
Michael A. Hollingsworth ◽  
Surinder K. Batra ◽  
Wen-Ning Qi ◽  
James R. Yankaskas
Keyword(s):  
Epithelial Cells ◽  
Mrna Expression ◽  
Bronchial Epithelial Cells ◽  
Bronchial Epithelial ◽  
Muc1 Mucin

scholarly journals Cl Transport in Complemented CF Bronchial Epithelial Cells Correlates with CFTR mRNA Expression Levels

2008 ◽  
Vol 22 (1-4) ◽  
pp. 057-068 ◽  
Author(s):  
Beate Illek ◽  
Rosalie Maurisse ◽  
Logan Wahler ◽  
Karl Kunzelmann ◽  
Horst Fischer ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Mrna Expression ◽  
Bronchial Epithelial Cells ◽  
Bronchial Epithelial ◽  
Expression Levels ◽  
Cl Transport ◽  
Mrna Expression Levels

Carbon black nanoparticles induce HDAC6-mediated inflammatory responses in 16HBE cells

2020 ◽  
Vol 36 (10) ◽  
pp. 759-768 ◽  
Author(s):  
Hui Lin ◽  
Guoqing Fu ◽  
Qimei Yu ◽  
Zhenyu Wang ◽  
Yao Zuo ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Carbon Black ◽  
Mrna Expression ◽  
Inflammatory Responses ◽  
Intraflagellar Transport ◽  
Bronchial Epithelial Cells ◽  
Bronchial Epithelial ◽  
Transfected Cells ◽  
Carbon Black Nanoparticles ◽  
Tnf Α

Long-term inhalation of carbon black nanoparticles (CBNPs) leads to pulmonary inflammatory diseases. Histone deacetylase 6 (HDAC6) has been identified as an important regulator in the development of inflammatory disorders. However, the direct involvement of HDAC6 in CBNPs-induced pulmonary inflammatory responses remains unclear. To explore whether HDAC6 participates in CBNPs-induced pulmonary inflammation, human bronchial epithelial cell line (16HBE cells) was transfected with HDAC6 small interference RNA (siRNA) and then exposed to CBNPs at concentrations of 0, 25, and 50 µg/ml for 24 h. Intracellular HDAC6 and intraflagellar transport protein 88 (IFT88) mRNA and protein were determined by real-time polymerase chain reaction and Western blot, respectively. The secretions of inflammatory cytokines including interleukin (IL)-8, tumor necrosis factor (TNF)-α, IL-6, and IL-1β were measured by enzyme-linked immunosorbent assay. CBNPs induced a significant increase in the expressions of IL-8 and IL-6, accompanied by a high level of intracellular HDAC6 mRNA when compared with a blank control group ( p < 0.05). However, there were no significant changes in the levels of TNF-α secretion, intracellular HDAC6 and IFT88 protein induced by CBNPs ( p > 0.05). The HDAC6 mRNA expression was significantly suppressed in HDAC6 siRNA-transfected cells ( p < 0.05). The secretions of IL-8, TNF-α, and IL-6 were significantly less in HDAC6 siRNA-transfected cells than that in normal 16HBE cells with exposure to 25 or 50 µg/ml of CBNPs, but intracellular IFT88 mRNA expression was markedly increased in HDAC6 siRNA-transfected cells when compared with normal 16HBE cells exposed to 50 µg/ml of CBNPs (all p < 0.05). Downregulation of the HDAC6 gene inhibits CBNPs-induced inflammatory responses in bronchial epithelial cells, partially through regulating IFT88 expression. It is suggested that CBNPs may trigger inflammatory responses in bronchial epithelial cells by an HDAC6/IFT88-dependent pathway.

scholarly journals Changes in microRNA and mRNA Expression with Differentiation of Human Bronchial Epithelial Cells

2013 ◽  
Vol 49 (3) ◽  
pp. 384-395 ◽  
Author(s):  
Asuncion Martinez-Anton ◽  
Milena Sokolowska ◽  
Steven Kern ◽  
A. Sally Davis ◽  
Sara Alsaaty ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Mrna Expression ◽  
Bronchial Epithelial Cells ◽  
Human Bronchial Epithelial Cells ◽  
Bronchial Epithelial
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