Localization of Hepatitis A Virus Antigen to Specific Subcellular Fractions of Hepatitis-A-Infected Chimpanzee Liver Cells

Intervirology ◽  
1984 ◽  
Vol 21 (4) ◽  
pp. 187-194 ◽  
Author(s):  
Narayan C. Khan ◽  
Blaine Hollinger ◽  
Joseph L. Melnick
Keyword(s):  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Liver Cells ◽  
Virus Antigen ◽  
Subcellular Fractions ◽  
Infected Chimpanzee

Antiviral activity of recombinant interferon-α on hepatitis A virus replication in human liver cells

1995 ◽  
Vol 28 (1) ◽  
pp. 69-80 ◽  
Author(s):  
Jean-Marc Crance ◽  
Françoise Lévêque ◽  
Suzanne Chousterman ◽  
Alain Jouan ◽  
Christian Trépo ◽  
...  
Keyword(s):  
Antiviral Activity ◽  
Virus Replication ◽  
Human Liver ◽  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Liver Cells ◽  
Interferon Α ◽  
Recombinant Interferon ◽  
Human Liver Cells

scholarly journals Immunofluorescence of hepatitis A virus antigen in chimpanzees.

1978 ◽  
Vol 21 (2) ◽  
pp. 663-665 ◽  
Author(s):  
B L Murphy ◽  
J E Maynard ◽  
D W Bradley ◽  
J W Ebert ◽  
L R Mathiesen ◽  
...  
Keyword(s):  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Virus Antigen

Relapsing hepatitis in a child, associated with isolation of hepatitis A virus antigen from the liver

1988 ◽  
Vol 147 (3) ◽  
pp. 333-333 ◽  
Author(s):  
J. N. van den Anker ◽  
R. N. Sukhai ◽  
A. M. Dumas
Keyword(s):  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Virus Antigen

Expression of a hepatitis A virus antigen in Lactococcus lactis and Escherichia coli and evaluation of its immunogenicity

2013 ◽  
Vol 97 (10) ◽  
pp. 4333-4342 ◽  
Author(s):  
Aleš Berlec ◽  
Tadej Malovrh ◽  
Petra Zadravec ◽  
Andrej Steyer ◽  
Matjaž Ravnikar ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Lactococcus Lactis ◽  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Virus Antigen

scholarly journals Growth of Hepatitis A Virus in a Mouse Liver Cell Line

2005 ◽  
Vol 79 (5) ◽  
pp. 2950-2955 ◽  
Author(s):  
Dino A. Feigelstock ◽  
Peter Thompson ◽  
Gerardo G. Kaplan
Keyword(s):  
Growth Factors ◽  
Mouse Liver ◽  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Growth Conditions ◽  
Liver Cells ◽  
Nucleotide Sequence Analysis ◽  
Mouse Liver Cell ◽  
A Cell ◽  
Cells Cultured

ABSTRACT Hepatitis A virus (HAV) has been adapted to grow efficiently in primate and some nonprimate cell lines but not in cells of murine origin. To understand the inability of the virus to grow in mouse cells, we studied the replication of HAV in immortalized and nontransformed MMH-D3 mouse liver cells, which require growth factors and collagen to maintain their phenotype. HAV grew in MMH-D3 cells transfected with virion RNA but not in those infected with viral particles, indicating a cell entry block for HAV. However, MMH-D3 cells cultured under suboptimal conditions in the absence of growth factors acquired susceptibility to HAV infection. Serial passages of the virus in MMH-D3 cells under suboptimal growth conditions resulted in the selection of HAV variants that grew efficiently in MMH-D3 cells cultured under both optimal and suboptimal conditions. Nucleotide sequence analysis of the MMH-D3 cell-adapted HAV revealed that N1237D and D2132G substitutions were present in the capsid regions of six viral clones. These two mutations are most likely located on the surface of the virion and may play a role in the entry of HAV into the mouse liver cells. Our results demonstrate that mouse hepatocyte-like cells code for all factors required for the efficient growth of HAV in cell culture.

Sign in / Sign up

Export Citation Format

Share Document