Relapsing hepatitis in a child, associated with isolation of hepatitis A virus antigen from the liver

1988 ◽  
Vol 147 (3) ◽  
pp. 333-333 ◽  
Author(s):  
J. N. van den Anker ◽  
R. N. Sukhai ◽  
A. M. Dumas
Keyword(s):  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Virus Antigen

scholarly journals Immunofluorescence of hepatitis A virus antigen in chimpanzees.

1978 ◽  
Vol 21 (2) ◽  
pp. 663-665 ◽  
Author(s):  
B L Murphy ◽  
J E Maynard ◽  
D W Bradley ◽  
J W Ebert ◽  
L R Mathiesen ◽  
...  
Keyword(s):  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Virus Antigen

Expression of a hepatitis A virus antigen in Lactococcus lactis and Escherichia coli and evaluation of its immunogenicity

2013 ◽  
Vol 97 (10) ◽  
pp. 4333-4342 ◽  
Author(s):  
Aleš Berlec ◽  
Tadej Malovrh ◽  
Petra Zadravec ◽  
Andrej Steyer ◽  
Matjaž Ravnikar ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Lactococcus Lactis ◽  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Virus Antigen

Dissociation of hepatitis a virus antigen-anti-hav antibody complexes by 2-mercaptoethanol and dithiothreitol

1982 ◽  
Vol 9 (4) ◽  
pp. 311-325 ◽  
Author(s):  
Daniel W. Bradley ◽  
Karen A. McCaustland ◽  
E.H. Cook ◽  
Howard A. Fields ◽  
Gert G. Frosner ◽  
...  
Keyword(s):  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Virus Antigen

scholarly journals Advances for the Hepatitis A Virus Antigen Production Using a Virus Strain With Codon Frequency Optimization Adjustments in Specific Locations

2021 ◽  
Vol 12 ◽  
Author(s):  
Gemma Chavarria-Miró ◽  
Montserrat de Castellarnau ◽  
Cristina Fuentes ◽  
Lucía D’Andrea ◽  
Francisco-Javier Pérez-Rodríguez ◽  
...  
Keyword(s):  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Virus Production ◽  
Vero Cells ◽  
Virus Antigen ◽  
Translation Efficiency ◽  
Entry Site ◽  
Internal Ribosome Entry ◽  
Fast Growing ◽  
Codon Composition

The available cell-adapted hepatitis A virus (HAV) strains show a very slow replication phenotype hampering the affordable production of antigen. A fast-growing strain characterized by the occurrence of mutations in the internal ribosome entry site (IRES), combined with changes in the codon composition has been selected in our laboratory. A characterization of the IRES activity of this fast-growing strain (HM175-HP; HP) vs. its parental strain (HM175; L0) was assessed in two cell substrates used in vaccine production (MRC-5 and Vero cells) compared with the FRhK-4 cell line in which its selection was performed. The HP-derived IRES was significantly more active than the L0-derived IRES in all cells tested and both IRES were more active in the FRhK-4 cells. The translation efficiency of the HP-derived IRES was also much higher than the L0-derived IRES, particularly, in genes with a HP codon usage background. These results correlated with a higher virus production in a shorter time for the HP strain compared to the L0 strain in any of the three cell lines tested, and of both strains in the FRhK-4 cells compared to Vero and MRC-5 cells. The addition of wortmannin resulted in the increase of infectious viruses and antigen in the supernatant of FRhK-4 infected cells, independently of the strain. Finally, the replication of both strains in a clone of FRhK-4 cells adapted to grow with synthetic sera was optimal and again the HP strain showed higher yields.

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