Development of the Adrenergic Innervation in the Myocardium and Coronary Arteries of the Dog

1990 ◽  
Vol 139 (2) ◽  
pp. 191-200 ◽  
Author(s):  
S. Doležel ◽  
M. Gerová ◽  
B. Hartmannová ◽  
J. Vašků
Keyword(s):  
Coronary Arteries ◽  
Adrenergic Innervation

Adrenergic innervation of the coronary arteries and the myocardium

1978 ◽  
Vol 100 (3) ◽  
pp. 306-316 ◽  
Author(s):  
S. Doležel ◽  
M. Gerová ◽  
J. Gero ◽  
T. Sládek ◽  
J. Vašku
Keyword(s):  
Coronary Arteries ◽  
Adrenergic Innervation

Automic innervation of dog coronary arteries

1976 ◽  
Vol 41 (1) ◽  
pp. 30-35 ◽  
Author(s):  
M. J. Denn ◽  
H. L. Stone
Keyword(s):  
Coronary Arteries ◽  
Stellate Ganglion ◽  
Coronary Vessels ◽  
Adrenergic Innervation ◽  
Cholinergic Innervation ◽  
Experimental Protocol ◽  
Cardiac Denervation ◽  
Intrinsic Nature ◽  
Great Vessels ◽  
Cervical Vagotomy

The autonomic innervation of canine coronary arteries has been examined using the Falck and Owman technique for demonstrating catecholamines and a modification of the Koelle technique for the demonstration of cholinesterase.The experimental protocol included an examination of the neural innervation of the major coronary arteries: LCC, LAD, and RCA. A consistent, relatively dense adrenergic innervation was noted. A gradient in the degree of cholinergic innervation was: LAD less than RCA less than LCC. Light microscopic examination of the hearts of dogs subjected to either cervical vagotomy or total extrinsic cardiac denervation was performed. Additional surgical procedures included removal of the left stellate ganglion and a preferentialstripping of the LCC. These studies demonstrated the intrinsic nature of parasympathetic coronary innervation. Following all surgical procedure no variations in density of cholinergic innervation were noted, indicating that these fibers are probably postganglionic parasympathetic fibers arising fromintrinsic ganglia within the ventricles. These ganglia may be located at thebase of the great vessels and send their fibers to the coronary vessels viathe septal artery.

Application of stereological analysis of cell volume to isolated myocytes in culture with and without adrenergic innervation

1990 ◽  
Vol 48 (3) ◽  
pp. 416-417
Author(s):  
Jane K. Rosenthal ◽  
Dianne L. Atkins ◽  
William J. Marvin ◽  
Penny A. Krumm
Keyword(s):  
Cardiac Myocytes ◽  
Structural Changes ◽  
Three Dimensional ◽  
Adrenergic Innervation ◽  
Culture Cell ◽  
Serial Sections ◽  
Newborn Rats ◽  
Primary Culture Cell ◽  
Biological Studies ◽  
Cell Volumes

To comprehend structural changes in cardiac myocytes accompanying adrenergic innervation, it is essential that a three dimensional analysis be performed. To date, biological studies which utilize stereological methods have been limited to cells in tissue and in organs. Our laboratory has utilized current stereological techniques for measuring absolute volumes of individual myocytes in primary culture. Cell volumes are calculated for two distinct groups of cells at 96 hours in culture: isolated myocytes and myocytes innervated with adrenergic neurons (Figure 1).Cardiac myocytes are cultured from the ventricular apices of newborn rats. Cells are plated directly onto tissue culture dishes with or without preplated explants from the paravertebral thoracolumbar sympathetic chain. On day four cultures are photographed and marked for one-to-one cell location. Following conventional fixation and embeddment in eponate-12, the cells are relocated and mounted for microtomy. The cells are completely sectioned at 120nm in their parallel orientation to the surface of the dish (Figure 2). Serial sections are collected on formvar coated slotted grids and are recorded in sequence.

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